AB254214

Anti-CDNF antibody [EPR22334-99] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal CDNF antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

ARMETL1, CDNF, Cerebral dopamine neurotrophic factor, ARMET-like protein 1, Conserved dopamine neurotrophic factor

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (AB254214)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (AB254214)

Immunohistochemistry analysis of Human skeletal muscle using ab253238 at 1/100 dilution. Ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) secondary was used. Positive staining on human skeletal muscle. The section was incubated with ab253238 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins This data was developed using the same antibody clone in a different buffer formulation (ab253238).

Unknown

Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (AB254214)

CDNF was immunoprecipitated from 0.35 mg human skeletal muscle tissue lysate with ab253238 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab253238 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.

Lane 1 : Human skeletal muscle tissue lysate 10 μg (input).
Lane 2 : ab253238 IP in human skeletal muscle tissue lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab253238 in human skeletal muscle lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253238).

All lanes:

Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] (<a href='/en-us/products/primary-antibodies/cdnf-antibody-epr22334-99-ab253238'>ab253238</a>)

Predicted band size: 21 kDa

false

Unknown

Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (AB254214)

CDNF was immunoprecipitated from 0.35 mg human testis tissue lysate with ab253238 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab253238 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.

Lane 1 : Human testis tissue lysate 10 μg (input).
Lane 2 : ab253238 IP in human testis tissue lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab253238 in human testis tissue lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253238).

All lanes:

Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] (<a href='/en-us/products/primary-antibodies/cdnf-antibody-epr22334-99-ab253238'>ab253238</a>)

Predicted band size: 21 kDa

false

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (AB254214)

Immunohistochemistry analysis of rat heart using ab253238 at 1/100 dilution. Ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) secondary was used. Positive staining on rat heart. The section was incubated with ab253238 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins This data was developed using the same antibody clone in a different buffer formulation (ab253238).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (AB254214)

Immunohistochemistry analysis of (A) Heart tissue from wild-type C57BL/6JGpt mice (B) Heart tissue from CDNF knockout mice using ab253238 at 1/100. Positive staining on (A) Heart tissue from wild-type C57BL/6JGpt mice and no staining on (B) Heart tissue from CDNF knockout mice. Ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) secondary was used. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and CDNF-KO homozygous mice (Strain ID : T031749). The section was incubated with ab253238 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins This data was developed using the same antibody clone in a different buffer formulation (ab253238).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (AB254214)

Immunohistochemistry analysis of (A) Testis tissue from wild-type C57BL/6JGpt mice (B) Testis tissue from CDNF knockout mice using ab253238 at 1/100. Positive staining on (A) Heart tissue from wild-type C57BL/6JGpt mice and no staining on (B) Heart tissue from CDNF knockout mice. Ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) secondary was used. Positive staining on (A) Testis tissue from wild-type C57BL/6JGpt mice and no staining on (B) Testis tissue from CDNF knockout mice. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and CDNF-KO homozygous mice (Strain ID : T031749). The section was incubated with ab253238 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrumenteval Solution2) for 20 mins This data was developed using the same antibody clone in a different buffer formulation (ab253238).

Lab

Western blot - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (AB254214)

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and CDNF-KO homozygous mice (Strain ID : T031749). This data was developed using the same antibody clone in a different buffer formulation (ab253238).

All lanes:

Western blot - Anti-CDNF antibody [EPR22334-99] (<a href='/en-us/products/primary-antibodies/cdnf-antibody-epr22334-99-ab253238'>ab253238</a>) at 1/1000 dilution

Lane 1:

CDNF knockout mouse brain tissue lysate

Lane 2:

Wild-type mouse brain tissue lysate

Lane 3:

CDNF knockout mouse gastrocnemius muscle tissue lysate

Lane 4:

Wild-type mouse gastrocnemius muscle tissue lysate

Lane 5:

CDNF knockout mouse heart tissue lysate

Lane 6:

Wild-type mouse heart tissue lysate

Lane 7:

CDNF knockout mouse soleus muscle tissue lysate

Lane 8:

Wild-type mouse soleus muscle tissue lysate

Lane 9:

CDNF knockout mouse testis tissue lysate

Lane 10:

Wild-type mouse testis tissue lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 26s

Unconjugated

Anti-CDNF antibody [EPR22334-99]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22334-99

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab254214 is the carrier-free version of ab253238.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CDNF also known as Cerebral Dopamine Neurotrophic Factor is a protein that helps in maintaining the proper function of neurons. It has a mass of approximately 18 kDa. This protein is widely expressed in various tissues especially in the brain. CDNF maintains endoplasmic reticulum homeostasis and is involved in the stabilization of dopaminergic neurons. It does this by reducing stress in the endoplasmic reticulum and promoting cell survival mechanisms.

Biological function summary

The protein ensures the survival of neurons by modulating cellular stress pathways. CDNF is not known to be part of a larger protein complex. Instead its activity relies on its own structural configuration to influence stress responses and neuron protection. As a secreted protein it directly interacts with and influences cellular mechanisms within the extracellular space contributing to a healthy neuronal environment.

Pathways

CDNF interacts with at least oxidative stress response pathways and neuroprotection pathways. These pathways underpin its role in counteracting cellular stress related to neurodegeneration. For example CDNF shares a functional connection with proteins like MANF (Mesencephalic Astrocyte-derived Neurotrophic Factor) another neurotrophic factor involved in similar neuroprotective pathways enhancing neuronal survival and recovery from cellular stress.

Neurodegenerative conditions such as Parkinson's disease and Alzheimer's disease are major areas where CDNF shows relevance. In Parkinson's disease CDNF's neuroprotective role helps mitigate the loss of dopaminergic neurons. There is evidence linking CDNF with MANF which also exhibits protective roles in neuronal health. Both proteins aim to promote neuron survival amid progression of neurodegeneration associated with these diseases indicating their potential as therapeutic agents.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra (By similarity).

See full target information CDNF

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CDNF antibody [EPR22334-99] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (AB254214)

Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (AB254214)

Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (AB254214)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (AB254214)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (AB254214)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (AB254214)

Western blot - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (AB254214)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise