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AB234973

Anti-CDS2 antibody

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(1 Review)

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(1 Publication)

Rabbit Polyclonal CDS2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CDS2 aa 350 to C-terminus.

View Alternative Names

Phosphatidate cytidylyltransferase 2, CDP-DAG synthase 2, CDP-DG synthase 2, CDP-diacylglycerol synthase 2, CDP-diglyceride pyrophosphorylase 2, CDP-diglyceride synthase 2, CTP:phosphatidate cytidylyltransferase 2, CDS 2, CDS2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDS2 antibody (AB234973)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDS2 antibody (AB234973)

Paraffin-embedded human liver tissue stained for CDS2 with ab234973 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDS2 antibody (AB234973)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDS2 antibody (AB234973)

Paraffin-embedded human brain tissue stained for CDS2 with ab234973 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody.

Western blot - Anti-CDS2 antibody (AB234973)
  • WB

Supplier Data

Western blot - Anti-CDS2 antibody (AB234973)

All lanes:

Western blot - Anti-CDS2 antibody (ab234973) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 3:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 51 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CDS2 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95674

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CDS2 also known as CDP-diacylglycerol synthase 2 is an enzyme that plays an important role in lipid metabolism. This protein has a molecular mass of approximately 52 kDa. It catalyzes the conversion of phosphatidic acid to CDP-diacylglycerol which is an important precursor for the synthesis of many phospholipids. CDS2 is mainly expressed in tissues with high lipid turnover such as the liver brain and adipose tissues.
Biological function summary

CDS2 is involved in maintaining cellular membrane integrity and function. It participates in the synthesis of phosphatidylinositol which is a component of the membrane and contributes to cellular signaling. CDS2 does not act alone; it functions as part of larger lipid synthesis pathways although it does not form a specific complex with other proteins. Still it interacts functionally with enzymes involved in lipid biosynthesis to ensure efficient lipid production within the cell.

Pathways

CDS2 functionally interacts in the glycerophospholipid biosynthesis and phosphatidylinositol signaling pathway. These pathways are central to generating phosphatidylinositol and related molecules which are critical for cellular processes such as membrane trafficking and signal transduction. CDS2's activity connects it with other proteins like phosphatidylinositol synthase which further modifies products of CDS2's reaction in the cell.

Abnormalities in CDS2 expression or function have correlations with metabolic disorders such as non-alcoholic fatty liver disease. Altered lipid metabolism impacts liver function where CDS2 plays an important role. Dysregulation in lipid pathways involving CDS2 may also be linked to neurological disorders given its significant expression in brain tissues. Disease associations may involve proteins such as glycerol-3-phosphate acyltransferase as their activities are interconnected in maintaining lipid homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the conversion of phosphatidic acid (PA) to CDP-diacylglycerol (CDP-DAG), an essential intermediate in the synthesis of phosphatidylglycerol, cardiolipin and phosphatidylinositol (PubMed : 25375833). Exhibits specificity for the nature of the acyl chains at the sn-1 and sn-2 positions in the substrate, PA and the preferred acyl chain composition is 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid (PubMed : 25375833). Plays an important role in regulating the growth and maturation of lipid droplets which are storage organelles at the center of lipid and energy homeostasis (PubMed : 26946540, PubMed : 31548309).
See full target information CDS2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Human gene therapy 34:682-696 PubMed37376759

2023

An Engineered Adeno-Associated Virus Capsid Mediates Efficient Transduction of Pericytes and Smooth Muscle Cells of the Brain Vasculature.

Applications

Unspecified application

Species

Unspecified reactive species

Servio H Ramirez,Jonathan F Hale,Siobhan McCarthy,Christian L Cardenas,Kalpani N Udeni Galpayage Dona,Killian S Hanlon,Eloise Hudry,Demitri De La Cruz,Carrie Ng,Sabyasachi Das,Diane M Nguyen,Josette Nammour,Rachel E Bennett,Allison M Andrews,Patricia L Musolino,Casey A Maguire
View all publications
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