AB70829

Anti-CDT1/DUP antibody

Name: Anti-CDT1/DUP antibody (ab70829) | Abcam
Brand: Abcam Limited
SKU: AB70829
Price: 545 USD
Availability: InStock
Rating: 3 (4 reviews)

(4 Reviews)

(11 Publications)

Rabbit Polyclonal CDT1/DUP antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human CDT1.

View Alternative Names

DNA replication factor Cdt1, Double parked homolog, DUP, CDT1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDT1/DUP antibody (AB70829)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDT1/DUP antibody (AB70829)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human islet cell carcinoma tissue labelling CDT1/DUP with ab70829 at 1/1000 (1μg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-CDT1/DUP antibody (AB70829)

Detection of CDT1/DUP by Western Blot of Immunprecipitate.
ab70829 at 1μg/ml staining CDT1/DUP in HeLa whole cell lysate immunoprecipitated using ab70829 at 3μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

Secondary antibody anti-rabbit HRP (ab6721)
Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-CDT1/DUP antibody (ab70829)

Predicted band size: 60 kDa

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Unknown

Western blot - Anti-CDT1/DUP antibody (AB70829)

Secondary antibody - anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-CDT1/DUP antibody (ab70829) at 1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 60 kDa

Observed band size: 63 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human CDT1. The exact immunogen used to generate this antibody is proprietary information.

Q9H211

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2500", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab70829 was affinity purified using an epitope specific to CDT1/DUP immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CDT1 also known as DUP or CDT-1 is an important regulator in cell cycle control. CDT1 has a molecular weight of approximately 68 kDa and is expressed predominantly in the nucleus during the late M and early G1 phases of the cell cycle. This protein plays an essential role in the licensing of DNA replication origins ensuring that DNA replication occurs precisely once per cell cycle. The careful regulation of CDT1 activity is necessary to prevent re-replication which can lead to genomic instability.

Biological function summary

This protein is necessary for the correct assembly of the pre-replicative complex (pre-RC) on replication origins. CDT1 works closely with CDC6 and the minichromosome maintenance (MCM) complex to form the pre-RC facilitating the loading of MCM helicase onto the DNA. This process tightly regulates replication origin licensing and ensures DNA replication fidelity. Misregulation of CDT1 can disrupt cell cycle progression and compromise genomic integrity.

Pathways

CDT1 functions primarily in cell cycle regulation and DNA replication. It participates in the origin recognition complex (ORC) pathway that initiates DNA replication. The proper function of CDT1 is critical to these pathways working alongside other proteins such as ORC1 and MCM complex members. Together they contribute to the accurate duplication of the genome which is pivotal for cell proliferation and maintenance of chromosomal stability.

CDT1 dysregulation associates with cancer and genomic instability. Overexpression or uncontrolled activity of CDT1 can lead to re-replication increasing the risk of mutations and chromosomal aberrations characteristic of neoplastic transformations. The protein's relationship with MCM components particularly those involved in cancer-related pathways highlights its relevance in tumorigenesis. Understanding CDT1's role in these diseases guides the development of targeted therapies and diagnostic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for both DNA replication and mitosis (PubMed : 11125146, PubMed : 14993212, PubMed : 21856198, PubMed : 22581055, PubMed : 26842564). DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC) (PubMed : 14672932). Required also for mitosis by promoting stable kinetochore-microtubule attachments (PubMed : 22581055). Potential oncogene (By similarity).

See full target information CDT1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:5076 PubMed37604829

2023

NFIB facilitates replication licensing by acting as a genome organizer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Zhang,Yue Wang,Yongjie Liu,Cuifang Liu,Yizhou Wang,Lin He,Xiao Cheng,Yani Peng,Lu Xia,Xiaodi Wu,Jiajing Wu,Yu Zhang,Luyang Sun,Ping Chen,Guohong Li,Qiang Tu,Jing Liang,Yongfeng Shang

Nature 592:789-793 PubMed33854235

2021

CRL4 is a master regulator of D-type cyclins.

Applications

Unspecified application

Species

Unspecified reactive species

Daniele Simoneschi,Gergely Rona,Nan Zhou,Yeon-Tae Jeong,Shaowen Jiang,Giacomo Milletti,Arnaldo A Arbini,Alfie O'Sullivan,Andrew A Wang,Sorasicha Nithikasem,Sarah Keegan,Yik Siu,Valentina Cianfanelli,Emiliano Maiani,Francesca Nazio,Francesco Cecconi,Francesco Boccalatte,David Fenyö,Drew R Jones,Luca Busino,Michele Pagano

Oncotarget 11:2512-2530 PubMed32655837

2020

Inhibition of the RUNX1-CBFβ transcription factor complex compromises mammary epithelial cell identity: a phenotype potentially stabilized by mitotic gene bookmarking.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua T Rose,Eliana Moskovitz,Joseph R Boyd,Jonathan A Gordon,Nicole A Bouffard,Andrew J Fritz,Anuradha Illendula,John H Bushweller,Jane B Lian,Janet L Stein,Sayyed K Zaidi,Gary S Stein

Molecular oncology 10:1196-206 PubMed27296872

2016

USP37 deubiquitinates Cdt1 and contributes to regulate DNA replication.

Applications

Species

Human

Santiago Hernández-Pérez,Elisa Cabrera,Hugo Amoedo,Sara Rodríguez-Acebes,Stephane Koundrioukoff,Michelle Debatisse,Juan Méndez,Raimundo Freire

Molecular and cellular biology 36:615-27 PubMed26644406

2015

Genome-Wide Studies Reveal that H3K4me3 Modification in Bivalent Genes Is Dynamically Regulated during the Pluripotent Cell Cycle and Stabilized upon Differentiation.

Applications

Species

Human

Rodrigo A Grandy,Troy W Whitfield,Hai Wu,Mark P Fitzgerald,Jennifer J VanOudenhove,Sayyed K Zaidi,Martin A Montecino,Jane B Lian,André J van Wijnen,Janet L Stein,Gary S Stein

Cell death & disease 5:e1351 PubMed25058425

2014

Immortalised breast epithelia survive prolonged DNA replication stress and return to cycle from a senescent-like state.

Applications

Species

Human

A Maya-Mendoza,J M Merchut-Maya,J Bartkova,J Bartek,C H Streuli,D A Jackson

Methods in molecular biology (Clifton, N.J.) 1170:367-82 PubMed24906324

2014

PCNA-dependent ubiquitination of Cdt1 and p21 in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Akiyo Hayashi,Naohiro Suenaga,Yasushi Shiomi,Hideo Nishitani

The Journal of pathology 233:113-23 PubMed24648314

2014

Lung tumourigenesis in a conditional Cul4A transgenic mouse model.

Applications

IHC-P

Species

Unspecified reactive species

Yi-Lin Yang,Ming-Szu Hung,Yang Wang,Jian Ni,Jian-Hua Mao,David Hsieh,Alfred Au,Atul Kumar,David Quigley,Li Tai Fang,Che-Chung Yeh,Zhidong Xu,David M Jablons,Liang You

Journal of cell science 127:1336-45 PubMed24434580

2014

PIP-box-mediated degradation prohibits re-accumulation of Cdc6 during S phase.

Applications

Unspecified application

Species

Unspecified reactive species

Linda Clijsters,Rob Wolthuis

The Journal of cell biology 201:1013-26 PubMed23775192

2013

The spindle checkpoint, APC/C(Cdc20), and APC/C(Cdh1) play distinct roles in connecting mitosis to S phase.

Applications

Unspecified application

Species

Human

Linda Clijsters,Janneke Ogink,Rob Wolthuis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CDT1/DUP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDT1/DUP antibody (AB70829)

Immunoprecipitation - Anti-CDT1/DUP antibody (AB70829)

Western blot - Anti-CDT1/DUP antibody (AB70829)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise