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Rabbit Recombinant Monoclonal CDT1/DUP antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 17 publications.


Images

Western blot - Anti-CDT1/DUP antibody [EPR17891] (AB202067), expandable thumbnail
  • Immunoprecipitation - Anti-CDT1/DUP antibody [EPR17891] (AB202067), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CDT1/DUP antibody [EPR17891] (AB202067), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDT1/DUP antibody [EPR17891] (AB202067), expandable thumbnail
  • Western blot - Anti-CDT1/DUP antibody [EPR17891] (AB202067), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-P
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Required for both DNA replication and mitosis (PubMed:11125146, PubMed:14993212, PubMed:21856198, PubMed:22581055, PubMed:26842564). DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC) (PubMed:14672932). Required also for mitosis by promoting stable kinetochore-microtubule attachments (PubMed:22581055). Potential oncogene (By similarity).

Alternative names

DNA replication factor Cdt1, Double parked homolog, DUP, CDT1

Recommended products

Rabbit Recombinant Monoclonal CDT1/DUP antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 17 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17891
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CDT1 also known as DUP or CDT-1 is an important regulator in cell cycle control. CDT1 has a molecular weight of approximately 68 kDa and is expressed predominantly in the nucleus during the late M and early G1 phases of the cell cycle. This protein plays an essential role in the licensing of DNA replication origins ensuring that DNA replication occurs precisely once per cell cycle. The careful regulation of CDT1 activity is necessary to prevent re-replication which can lead to genomic instability.

Biological function summary

This protein is necessary for the correct assembly of the pre-replicative complex (pre-RC) on replication origins. CDT1 works closely with CDC6 and the minichromosome maintenance (MCM) complex to form the pre-RC facilitating the loading of MCM helicase onto the DNA. This process tightly regulates replication origin licensing and ensures DNA replication fidelity. Misregulation of CDT1 can disrupt cell cycle progression and compromise genomic integrity.

Pathways

CDT1 functions primarily in cell cycle regulation and DNA replication. It participates in the origin recognition complex (ORC) pathway that initiates DNA replication. The proper function of CDT1 is critical to these pathways working alongside other proteins such as ORC1 and MCM complex members. Together they contribute to the accurate duplication of the genome which is pivotal for cell proliferation and maintenance of chromosomal stability.

Associated diseases and disorders

CDT1 dysregulation associates with cancer and genomic instability. Overexpression or uncontrolled activity of CDT1 can lead to re-replication increasing the risk of mutations and chromosomal aberrations characteristic of neoplastic transformations. The protein's relationship with MCM components particularly those involved in cancer-related pathways highlights its relevance in tumorigenesis. Understanding CDT1's role in these diseases guides the development of targeted therapies and diagnostic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-CDT1/DUP antibody [EPR17891] (ab202067), expandable thumbnail

    Western blot - Anti-CDT1/DUP antibody [EPR17891] (ab202067)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CDT1/DUP antibody [EPR17891] (ab202067) at 1/10000 dilution

    All lanes: HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 47 kDa, 60 kDa

    Observed band size: 50-70 kDa, 60 kDa

    Exposure time: 1min

  • Immunoprecipitation - Anti-CDT1/DUP antibody [EPR17891] (ab202067), expandable thumbnail

    Immunoprecipitation - Anti-CDT1/DUP antibody [EPR17891] (ab202067)

    CDT1/DUP was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab202067 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab202067 at 1/1000 dilution.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.

    Lane 1: HEK-293 whole cell lysate 10 μg (Input).

    Lane 2: ab202067 IP in HEK-293 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab202067 in HEK-293 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    All lanes: Immunoprecipitation - Anti-CDT1/DUP antibody [EPR17891] (ab202067)

    Predicted band size: 60 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CDT1/DUP antibody [EPR17891] (ab202067), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CDT1/DUP antibody [EPR17891] (ab202067)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CDT1/DUP with ab202067 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green).

    Confocal image showing nuclear staining on HeLa cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202067 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDT1/DUP antibody [EPR17891] (ab202067), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDT1/DUP antibody [EPR17891] (ab202067)

    Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue labeling CDT1/DUP with ab202067 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.

    Nuclear staining on human cervix carcinoma tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-CDT1/DUP antibody [EPR17891] (ab202067), expandable thumbnail

    Western blot - Anti-CDT1/DUP antibody [EPR17891] (ab202067)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CDT1/DUP antibody [EPR17891] (ab202067) at 1/2000 dilution

    All lanes: HEK-293 (Human epithelial cells from embryonic kidney) cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 60 kDa

    Observed band size: 60 kDa

    Exposure time: 1min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDT1/DUP antibody [EPR17891] (ab202067), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDT1/DUP antibody [EPR17891] (ab202067)

    Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling CDT1/DUP with ab202067 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.

    Nuclear staining on Human colonic carcinoma tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CDT1/DUP antibody [EPR17891] (ab202067), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CDT1/DUP antibody [EPR17891] (ab202067)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cells) cells labeling CDT1/DUP with ab202067 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green).

    Confocal image showing nuclear staining on T-29 cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202067 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Western blot - Anti-CDT1/DUP antibody [EPR17891] (ab202067), expandable thumbnail

    Western blot - Anti-CDT1/DUP antibody [EPR17891] (ab202067)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CDT1/DUP antibody [EPR17891] (ab202067) at 1/1000 dilution

    All lanes: Human fetal lung lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 60 kDa

    Observed band size: 60 kDa

    Exposure time: 1min

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Product protocols

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