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AB326693

Anti-CDT1/DUP antibody [SR3533]

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Rabbit Recombinant Monoclonal CDT1/DUP antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.

View Alternative Names

DNA replication factor Cdt1, Double parked homolog, DUP, CDT1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDT1/DUP antibody [SR3533] (AB326693)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDT1/DUP antibody [SR3533] (AB326693)

Formalin-fixed, paraffin-embedded human colon tissue stained for CDT1/DUP using antibody at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-CDT1/DUP antibody [SR3533] (AB326693)
  • WB

Supplier Data

Western blot - Anti-CDT1/DUP antibody [SR3533] (AB326693)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Hela whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 60 kDa
Observed band size : 60 kDa

Exposure time : 5 seconds

All lanes:

Western blot - Anti-CDT1/DUP antibody [SR3533] (ab326693) at 1/1000 dilution

Lane 1:

Hela whole cell lysates at 20 µg

Lane 2:

JK whole cell lysates at 20 µg

Lane 3:

MCF-7 whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

Exposure time: 5s

Western blot - Anti-CDT1/DUP antibody [SR3533] (AB326693)
  • WB

Supplier Data

Western blot - Anti-CDT1/DUP antibody [SR3533] (AB326693)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : CDT1 Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.

All lanes:

Western blot - Anti-CDT1/DUP antibody [SR3533] (ab326693) at 1/1000 dilution

Lane 1:

Wild-type Hela cell lysate at 20 µg

Lane 2:

CDT1 Rabbit mAb knockdown Hela cell lysate at 20 µg

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR3533

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for both DNA replication and mitosis (PubMed : 11125146, PubMed : 14993212, PubMed : 21856198, PubMed : 22581055, PubMed : 26842564). DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC) (PubMed : 14672932). Required also for mitosis by promoting stable kinetochore-microtubule attachments (PubMed : 22581055). Potential oncogene (By similarity).
See full target information CDT1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com