AB174385

Anti-CDT2/RAMP antibody

Name: Anti-CDT2/RAMP antibody (ab174385) | Abcam
Brand: Abcam Limited
SKU: AB174385
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal CDT2/RAMP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DTL.

View Alternative Names

CDT2, CDW1, DCAF2, L2DTL, RAMP, DTL, Denticleless protein homolog, DDB1- and CUL4-associated factor 2, Lethal(2) denticleless protein homolog, Retinoic acid-regulated nuclear matrix-associated protein

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-CDT2/RAMP antibody (AB174385)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CDT2/RAMP antibody (AB174385)

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labelling CDT2/RAMP with ab174385 at 1/100 dilution (Red). The cells were stained for DNA with Hoechst 33258 (Blue). CDT2 protein is localized in nuclei at all cell stages.

Supplier Data

Western blot - Anti-CDT2/RAMP antibody (AB174385)

Inhibition of CDT2/RAMP protein synthesis by CDT2/RAMP siRNA introduced into HeLa cells. siCont is control siRNA unrelated to CDT2/RAMP. siCDT2 is CDT2/RAMP specific siRNA.

CDT2/RAMP is phosphorylated after UV irradiation as shown by the band shift-up in irradiated sample (UV +).

All lanes:

Western blot - Anti-CDT2/RAMP antibody (ab174385)

Predicted band size: 79 kDa

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Supplier Data

Western blot - Anti-CDT2/RAMP antibody (AB174385)

All lanes:

Western blot - Anti-CDT2/RAMP antibody (ab174385) at 1/2000 dilution

All lanes:

HeLa whole cell extract at 20 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/20000 dilution

Predicted band size: 79 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human DTL. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

pH: 6 - 8.5 Preservative: 0.05% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CDT2 also known as RAMP (Rbx1-Associated Cullin 4-DCAF12-Dependent E3 Ligase) is part of the CRL4 complex and serves as a substrate recognition component for E3 ubiquitin ligases. The molecular weight of the CDT2 protein is approximately 85 kDa. This protein shows expression in various tissues with higher levels in actively dividing cells like those found in the testes and thymus.

Biological function summary

CDT2 regulates cell cycle progression by targeting specific cell cycle regulators for ubiquitin-mediated degradation. It functions within the CRL4 complex where it helps to ensure the timely turnover of replication licensing factors such as Cdt1 and other proteins involved in cell cycle checkpoints. This regulation is important for maintaining genomic stability by preventing inappropriate replication origin firing.

Pathways

CDT2 plays a significant role in DNA replication and cell cycle control pathways. Within these pathways the protein coordinates with Cdt1 to prevent unscheduled DNA replication. CDT2's involvement in these processes links it closely to proteins such as CUL4 which forms part of the CRL4 complex and to the ubiquitin-proteasome system a critical pathway for protein homeostasis.

CDT2 has implications in cancer and genomic instability. Aberrations in CDT2 expression or function can lead to dysregulation of cell proliferation contributing to oncogenesis. Connections between CDT2 and cancer are emphasized by its interaction with substrates like Cdt1 where misregulation may drive the development of tumors. CDT2 is also connected to p21 through its influence on cell cycle checkpoints highlighting an additional role in tumor suppression pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1), FBH1, KMT5A and SDE2 (PubMed : 16861906, PubMed : 16949367, PubMed : 16964240, PubMed : 17085480, PubMed : 18703516, PubMed : 18794347, PubMed : 18794348, PubMed : 19332548, PubMed : 20129063, PubMed : 23478441, PubMed : 23478445, PubMed : 23677613, PubMed : 27906959). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication (PubMed : 16861906, PubMed : 16949367, PubMed : 17085480). CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing (PubMed : 18794348, PubMed : 19332548). KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration (PubMed : 23478445). Most substrates require their interaction with PCNA for their polyubiquitination : substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis (PubMed : 20129063, PubMed : 23478441, PubMed : 23478445, PubMed : 23677613). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed : 26431207).

See full target information DTL

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:476 PubMed37809049

2023

Osteopontin regulates the growth and invasion of liver cancer cells via DTL.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyong Liu,Guang Yang,Xiaoyu Yi,Shijie Zhang,Zhibo Feng,Xudong Cui,Feilong Chen,Lei Yu

The Journal of biological chemistry 283:29045-52 PubMed18703516

2008

CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation.

Applications

WB, Fluorescent Cell Imaging

Species

Human, Human

Hideo Nishitani,Yasushi Shiomi,Hiroka Iida,Masato Michishita,Toshihiro Takami,Toshiki Tsurimoto

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CDT2/RAMP antibody

Immunocytochemistry/ Immunofluorescence - Anti-CDT2/RAMP antibody (AB174385)

Western blot - Anti-CDT2/RAMP antibody (AB174385)

Western blot - Anti-CDT2/RAMP antibody (AB174385)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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