Rabbit Recombinant Monoclonal CDT2/RAMP antibody. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (AB306556), expandable thumbnail](https://content.abcam.com/products/images/cdt2-ramp-antibody-epr26845-75-ab306556--western-blot-img405774.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (AB306556)")
![Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (AB306556), expandable thumbnail](https://content.abcam.com/products/images/cdt2-ramp-antibody-epr26845-75-ab306556--western-blot-img405462.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (AB306556)")
![Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (AB306556), expandable thumbnail](https://content.abcam.com/products/images/cdt2-ramp-antibody-epr26845-75-ab306556--western-blot-img405461.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (AB306556)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Not suitable for mouse and rat. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Not suitable for mouse and rat. |
Species Rat | Dilution info - | Notes Not suitable for mouse and rat. |
Target data
Function
Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1), FBH1, KMT5A and SDE2 (PubMed:16861906, PubMed:16949367, PubMed:16964240, PubMed:17085480, PubMed:18703516, PubMed:18794347, PubMed:18794348, PubMed:19332548, PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613, PubMed:27906959). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication (PubMed:16861906, PubMed:16949367, PubMed:17085480). CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing (PubMed:18794348, PubMed:19332548). KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration (PubMed:23478445). Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis (PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed:26431207).
Alternative names
CDT2, CDW1, DCAF2, L2DTL, RAMP, DTL, Denticleless protein homolog, DDB1- and CUL4-associated factor 2, Lethal(2) denticleless protein homolog, Retinoic acid-regulated nuclear matrix-associated protein
Recommended products
Rabbit Recombinant Monoclonal CDT2/RAMP antibody. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR26845-75
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CDT2 also known as RAMP (Rbx1-Associated Cullin 4-DCAF12-Dependent E3 Ligase) is part of the CRL4 complex and serves as a substrate recognition component for E3 ubiquitin ligases. The molecular weight of the CDT2 protein is approximately 85 kDa. This protein shows expression in various tissues with higher levels in actively dividing cells like those found in the testes and thymus.
- Biological function summary
CDT2 regulates cell cycle progression by targeting specific cell cycle regulators for ubiquitin-mediated degradation. It functions within the CRL4 complex where it helps to ensure the timely turnover of replication licensing factors such as Cdt1 and other proteins involved in cell cycle checkpoints. This regulation is important for maintaining genomic stability by preventing inappropriate replication origin firing.
- Pathways
CDT2 plays a significant role in DNA replication and cell cycle control pathways. Within these pathways the protein coordinates with Cdt1 to prevent unscheduled DNA replication. CDT2's involvement in these processes links it closely to proteins such as CUL4 which forms part of the CRL4 complex and to the ubiquitin-proteasome system a critical pathway for protein homeostasis.
- Associated diseases and disorders
CDT2 has implications in cancer and genomic instability. Aberrations in CDT2 expression or function can lead to dysregulation of cell proliferation contributing to oncogenesis. Connections between CDT2 and cancer are emphasized by its interaction with substrates like Cdt1 where misregulation may drive the development of tumors. CDT2 is also connected to p21 through its influence on cell cycle checkpoints highlighting an additional role in tumor suppression pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (ab306556), expandable thumbnail](https://content.abcam.com/products/images/cdt2-ramp-antibody-epr26845-75-ab306556--western-blot-img405774.jpg "Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (ab306556)")
Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (ab306556)
Blocking and diluting buffer and concentration: 5% NFDM/TBSTThe expression of CDT2/RAMP is downregulated in response to PMA treatment (PMID: 24637302).
Samples are non-boiled as boiling may cause protein aggregation.
Exposure time: 59 secondsAll lanes: Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (ab306556) at 1/1000 dilution
Lane 1: Untreated K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 20 µg
Lane 2: K-562 treated with 20nM PMA for 4 days whole cell lysate 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 91 kDa
Exposure time: 59s
![Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (ab306556), expandable thumbnail](https://content.abcam.com/products/images/cdt2-ramp-antibody-epr26845-75-ab306556--western-blot-img405462.jpg "Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (ab306556)")
Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (ab306556)
Blocking and diluting buffer and concentration: 5% NFDM/TBSTLow expression: human heart (PMID: 18542055).
Samples are non-boiled as boiling may cause protein aggregation.
The identity of the bands between 20 kDa and 70 kDa are unknown.
Exposure time: 158 secondsAll lanes: Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (ab306556) at 1/1000 dilution
Lane 1: Human testis tissue lysate 20 μg
Lane 2: Human heart tissue lysate 20 μg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 91 kDa
Exposure time: 158s
![Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (ab306556), expandable thumbnail](https://content.abcam.com/products/images/cdt2-ramp-antibody-epr26845-75-ab306556--western-blot-img405461.jpg "Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (ab306556)")
Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (ab306556)
Blocking and diluting buffer and concentration: 5% NFDM/TBSTSamples are non-boiled as boiling may cause protein aggregation.
Exposure time: Lanes 1-5: 15 seconds, Lanes 6-7: 81 seconds.All lanes: Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (ab306556) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 91 kDa
Exposure time: 26s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com