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AB306556

Anti-CDT2/RAMP antibody [EPR26845-75]

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Rabbit Recombinant Monoclonal CDT2/RAMP antibody. Suitable for WB and reacts with Human samples.

View Alternative Names

CDT2, CDW1, DCAF2, L2DTL, RAMP, DTL, Denticleless protein homolog, DDB1- and CUL4-associated factor 2, Lethal(2) denticleless protein homolog, Retinoic acid-regulated nuclear matrix-associated protein

3 Images
Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (AB306556)
  • WB

Supplier Data

Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (AB306556)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTLow expression : human heart (PMID : 18542055). Samples are non-boiled as boiling may cause protein aggregation. The identity of the bands between 20 kDa and 70 kDa are unknown. Exposure time : 158 seconds

All lanes:

Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (ab306556) at 1/1000 dilution

Lane 1:

Human testis tissue lysate 20 μg

Lane 2:

Human heart tissue lysate 20 μg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 91 kDa

false

Exposure time: 158s

Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (AB306556)
  • WB

Supplier Data

Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (AB306556)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTThe expression of CDT2/RAMP is downregulated in response to PMA treatment (PMID : 24637302). Samples are non-boiled as boiling may cause protein aggregation. Exposure time : 59 seconds

All lanes:

Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (ab306556) at 1/1000 dilution

Lane 1:

Untreated K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 20 µg

Lane 2:

K-562 treated with 20nM PMA for 4 days whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 91 kDa

false

Exposure time: 59s

Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (AB306556)
  • WB

Supplier Data

Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (AB306556)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTSamples are non-boiled as boiling may cause protein aggregation. Exposure time : Lanes 1-5 : 15 seconds, Lanes 6-7 : 81 seconds.

All lanes:

Western blot - Anti-CDT2/RAMP antibody [EPR26845-75] (ab306556) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 91 kDa

false

Exposure time: 26s

  • Carrier free

    Anti-CDT2/RAMP antibody [EPR26845-75] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26845-75

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Primary Antibodies

AB72548

Anti-Cullin 4A/CUL-4A antibody

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CDT2 also known as RAMP (Rbx1-Associated Cullin 4-DCAF12-Dependent E3 Ligase) is part of the CRL4 complex and serves as a substrate recognition component for E3 ubiquitin ligases. The molecular weight of the CDT2 protein is approximately 85 kDa. This protein shows expression in various tissues with higher levels in actively dividing cells like those found in the testes and thymus.
Biological function summary

CDT2 regulates cell cycle progression by targeting specific cell cycle regulators for ubiquitin-mediated degradation. It functions within the CRL4 complex where it helps to ensure the timely turnover of replication licensing factors such as Cdt1 and other proteins involved in cell cycle checkpoints. This regulation is important for maintaining genomic stability by preventing inappropriate replication origin firing.

Pathways

CDT2 plays a significant role in DNA replication and cell cycle control pathways. Within these pathways the protein coordinates with Cdt1 to prevent unscheduled DNA replication. CDT2's involvement in these processes links it closely to proteins such as CUL4 which forms part of the CRL4 complex and to the ubiquitin-proteasome system a critical pathway for protein homeostasis.

CDT2 has implications in cancer and genomic instability. Aberrations in CDT2 expression or function can lead to dysregulation of cell proliferation contributing to oncogenesis. Connections between CDT2 and cancer are emphasized by its interaction with substrates like Cdt1 where misregulation may drive the development of tumors. CDT2 is also connected to p21 through its influence on cell cycle checkpoints highlighting an additional role in tumor suppression pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1), FBH1, KMT5A and SDE2 (PubMed : 16861906, PubMed : 16949367, PubMed : 16964240, PubMed : 17085480, PubMed : 18703516, PubMed : 18794347, PubMed : 18794348, PubMed : 19332548, PubMed : 20129063, PubMed : 23478441, PubMed : 23478445, PubMed : 23677613, PubMed : 27906959). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication (PubMed : 16861906, PubMed : 16949367, PubMed : 17085480). CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing (PubMed : 18794348, PubMed : 19332548). KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration (PubMed : 23478445). Most substrates require their interaction with PCNA for their polyubiquitination : substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis (PubMed : 20129063, PubMed : 23478441, PubMed : 23478445, PubMed : 23677613). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed : 26431207).
See full target information DTL
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com