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AB314022

Anti-Cdx1 antibody [11E10]

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Rabbit Recombinant Monoclonal CDX1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CDX1.

View Alternative Names

Homeobox protein CDX-1, Caudal-type homeobox protein 1, CDX1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cdx1 antibody [11E10] (AB314022)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cdx1 antibody [11E10] (AB314022)

Immunofluorescence staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells with ab314022 at 1 : 50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor® 524-conjugated Goat Anti-Rabbit IgG(H+L).

Western blot - Anti-Cdx1 antibody [11E10] (AB314022)
  • WB

Supplier Data

Western blot - Anti-Cdx1 antibody [11E10] (AB314022)

All lanes:

Western blot - Anti-Cdx1 antibody [11E10] (ab314022) at 1/2000 dilution

All lanes:

HepG2 whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 25-30 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

11E10

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human CDX1.

P47902

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdx1 also known as Caudal type homeobox 1 is a transcription factor with a molecular mass of approximately 32 kDa. This protein plays a significant role in the regulation of gene expression during embryonic development. Cdx1 mainly expresses in the intestinal epithelium and to some extent in the colon and rectum. It binds to specific DNA sequences influencing the expression of various genes related to development and differentiation.
Biological function summary

Regulating gene expression during development Cdx1 is important for maintaining the identity and function of intestinal cells. It does not operate on its own but is part of a complex network of transcription factors guiding the positional development and morphogenesis of the intestinal tract. This protein contributes to the proper development and specialization of tissues along the anterior-posterior axis of embryonic structures.

Pathways

Cdx1 interacts with several factors that make it an integral part of key developmental pathways like the Wnt signaling pathway and the Hox gene regulatory network. These pathways influence cellular differentiation and tissue patterning. In these pathways Cdx1 connects with other proteins such as beta-catenin and Hox genes cooperating in the spatial configuration of cellular structures during embryogenesis and adult tissue maintenance.

Cdx1 has been linked to colorectal cancer and other gut-related disorders due to its role in cell differentiation and proliferation. Abnormal Cdx1 expression leads to deregulated growth in intestinal epithelial cells contributing to oncogenesis. It interacts with other proteins involved in these disorders such as APC and p53 which can exacerbate or modulate the disease progression through disrupted signal pathways and altered cellular homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in transcriptional regulation (PubMed : 24623306). Involved in activated KRAS-mediated transcriptional activation of PRKD1 in colorectal cancer (CRC) cells (PubMed : 24623306). Binds to the PRKD1 promoter in colorectal cancer (CRC) cells (PubMed : 24623306). Could play a role in the terminal differentiation of the intestine. Binds preferentially to methylated DNA (PubMed : 28473536).
See full target information CDX1
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Product promise

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For full details, please see our Terms & Conditions

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