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AB224615

Anti-Cdx1 antibody

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(1 Publication)

Rabbit Polyclonal CDX1 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CDX1 aa 200-250.

View Alternative Names

Homeobox protein CDX-1, Caudal-type homeobox protein 1, CDX1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cdx1 antibody (AB224615)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cdx1 antibody (AB224615)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cdx1 (green) using ab224615 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-Cdx1 antibody (AB224615)
  • WB

Supplier Data

Western blot - Anti-Cdx1 antibody (AB224615)

All lanes:

Western blot - Anti-Cdx1 antibody (ab224615) at 1/500 dilution

All lanes:

Rat liver lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CDX1 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

P47902

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdx1 also known as Caudal type homeobox 1 is a transcription factor with a molecular mass of approximately 32 kDa. This protein plays a significant role in the regulation of gene expression during embryonic development. Cdx1 mainly expresses in the intestinal epithelium and to some extent in the colon and rectum. It binds to specific DNA sequences influencing the expression of various genes related to development and differentiation.
Biological function summary

Regulating gene expression during development Cdx1 is important for maintaining the identity and function of intestinal cells. It does not operate on its own but is part of a complex network of transcription factors guiding the positional development and morphogenesis of the intestinal tract. This protein contributes to the proper development and specialization of tissues along the anterior-posterior axis of embryonic structures.

Pathways

Cdx1 interacts with several factors that make it an integral part of key developmental pathways like the Wnt signaling pathway and the Hox gene regulatory network. These pathways influence cellular differentiation and tissue patterning. In these pathways Cdx1 connects with other proteins such as beta-catenin and Hox genes cooperating in the spatial configuration of cellular structures during embryogenesis and adult tissue maintenance.

Cdx1 has been linked to colorectal cancer and other gut-related disorders due to its role in cell differentiation and proliferation. Abnormal Cdx1 expression leads to deregulated growth in intestinal epithelial cells contributing to oncogenesis. It interacts with other proteins involved in these disorders such as APC and p53 which can exacerbate or modulate the disease progression through disrupted signal pathways and altered cellular homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in transcriptional regulation (PubMed : 24623306). Involved in activated KRAS-mediated transcriptional activation of PRKD1 in colorectal cancer (CRC) cells (PubMed : 24623306). Binds to the PRKD1 promoter in colorectal cancer (CRC) cells (PubMed : 24623306). Could play a role in the terminal differentiation of the intestine. Binds preferentially to methylated DNA (PubMed : 28473536).
See full target information CDX1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neoplasma 69:1349-1358 PubMed36305691

2022

PHF21A expression as a biomarker of hepatocellular carcinoma progression and prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Qing Huang,Li-Chen Ji,Qiang-An Jing,Yi-Chen He,Ying-Yu Ma,Xiang-Min Tong
View all publications
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