AB126748

Anti-Cdx1 antibody [EPR4819(2)]

Name: Anti-Cdx1 antibody [EPR4819(2)] (ab126748) | Abcam
Brand: Abcam Limited
SKU: AB126748
Price: 275 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal CDX1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Homeobox protein CDX-1, Caudal-type homeobox protein 1, CDX1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cdx1 antibody [EPR4819(2)] (AB126748)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cdx1 antibody [EPR4819(2)] (AB126748)

ab126748, at 1/100 dilution, staining Cdx1 in Caco-2 cells by Immunofluorescence.

Unknown

Western blot - Anti-Cdx1 antibody [EPR4819(2)] (AB126748)

All lanes:

Western blot - Anti-Cdx1 antibody [EPR4819(2)] (ab126748) at 1/1000 dilution

Lane 1:

Caco-2 lysate at 10 µg

Lane 2:

Human stomach lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 28 kDa

Observed band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4819(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdx1 also known as Caudal type homeobox 1 is a transcription factor with a molecular mass of approximately 32 kDa. This protein plays a significant role in the regulation of gene expression during embryonic development. Cdx1 mainly expresses in the intestinal epithelium and to some extent in the colon and rectum. It binds to specific DNA sequences influencing the expression of various genes related to development and differentiation.

Biological function summary

Regulating gene expression during development Cdx1 is important for maintaining the identity and function of intestinal cells. It does not operate on its own but is part of a complex network of transcription factors guiding the positional development and morphogenesis of the intestinal tract. This protein contributes to the proper development and specialization of tissues along the anterior-posterior axis of embryonic structures.

Pathways

Cdx1 interacts with several factors that make it an integral part of key developmental pathways like the Wnt signaling pathway and the Hox gene regulatory network. These pathways influence cellular differentiation and tissue patterning. In these pathways Cdx1 connects with other proteins such as beta-catenin and Hox genes cooperating in the spatial configuration of cellular structures during embryogenesis and adult tissue maintenance.

Cdx1 has been linked to colorectal cancer and other gut-related disorders due to its role in cell differentiation and proliferation. Abnormal Cdx1 expression leads to deregulated growth in intestinal epithelial cells contributing to oncogenesis. It interacts with other proteins involved in these disorders such as APC and p53 which can exacerbate or modulate the disease progression through disrupted signal pathways and altered cellular homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in transcriptional regulation (PubMed : 24623306). Involved in activated KRAS-mediated transcriptional activation of PRKD1 in colorectal cancer (CRC) cells (PubMed : 24623306). Binds to the PRKD1 promoter in colorectal cancer (CRC) cells (PubMed : 24623306). Could play a role in the terminal differentiation of the intestine. Binds preferentially to methylated DNA (PubMed : 28473536).

See full target information CDX1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 224: PubMed40552983

2025

Acidosis attenuates the hypoxic stabilization of HIF-1α by activating lysosomal degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Bobby White,Zhenyi Wang,Matthew Dean,Johanna Michl,Natalia Nieora,Sarah Flannery,Iolanda Vendrell,Roman Fischer,Alzbeta Hulikova,Pawel Swietach

Scientific reports 11:9892 PubMed33972635

2021

Endoplasmic reticulum stress regulates the intestinal stem cell state through CtBP2.

Applications

Unspecified application

Species

Unspecified reactive species

Bartolomeus J Meijer,Wouter L Smit,Pim J Koelink,Barbara F Westendorp,Ruben J de Boer,Jonathan H M van der Meer,Jacqueline L M Vermeulen,James C Paton,Adrienne W Paton,Jun Qin,Evelien Dekker,Vanesa Muncan,Gijs R van den Brink,Jarom Heijmans

Cancer biotherapy & radiopharmaceuticals : PubMed32876500

2020

MiR-24 Promotes Cell Growth in Human Glioma by /PI3K/Akt Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Lei,Huanhuan Sun,Bo Zhang

Molecular therapy. Nucleic acids 14:377-387 PubMed30710891

2019

The Attenuation of Trophoblast Invasion Caused by the Downregulation of EZH2 Is Involved in the Pathogenesis of Human Recurrent Miscarriage.

Applications

Unspecified application

Species

Unspecified reactive species

Shijian Lv,Na Wang,Hong Lv,Jieqiong Yang,Jianwei Liu,Wei-Ping Li,Cong Zhang,Zi-Jiang Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cdx1 antibody [EPR4819(2)]

Immunocytochemistry/ Immunofluorescence - Anti-Cdx1 antibody [EPR4819(2)] (AB126748)

Western blot - Anti-Cdx1 antibody [EPR4819(2)] (AB126748)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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