Mouse Monoclonal CDX2 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CDX2 aa 150-250.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [CDX2/1690] (AB217884), expandable thumbnail](https://content.abcam.com/products/images/cdx2-antibody-cdx2-1690-ab217884--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130114.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [CDX2/1690] (AB217884)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [CDX2/1690] (AB217884), expandable thumbnail](https://content.abcam.com/products/images/cdx2-antibody-cdx2-1690-ab217884--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62453.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [CDX2/1690] (AB217884)")
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human CDX2 aa 150-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q99626
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes (Primary incubation for 30 minutes at RT). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium (By similarity). Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription (By similarity). Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription (By similarity). Binds to and activates transcription of LPH (By similarity). Activates transcription of CLDN2 and intestinal mucin MUC2 (By similarity). Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription (By similarity). Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA (PubMed:28473536).
Alternative names
CDX3, CDX2, Homeobox protein CDX-2, CDX-3, Caudal-type homeobox protein 2
Recommended products
Mouse Monoclonal CDX2 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CDX2 aa 150-250.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human CDX2 aa 150-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q99626
- Clone number
- CDX2/1690
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
- Purification notes
ab217884 was purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CDX2 or Caudal Type Homeobox 2 is a transcription factor involved in intestinal development and differentiation. Alternative names for CDX2 include Cdx-2 and CDX-2. This protein has a molecular mass of approximately 38 kDa. CDX2 is expressed mainly in the epithelial cells of the intestine. Researchers can identify CDX2 through techniques like CDX2 stain CDX2 immunostain and CDX2 IHC which are useful for determining its expression in tissue samples.
- Biological function summary
CDX2 plays an important role in controlling the expression of genes necessary for maintaining intestinal epithelial cell identity. It functions as a part of regulatory networks that guide cell fate and differentiation in the gut. CDX2 does not generally form complexes but acts as a transcriptional regulator that binds directly to DNA influencing genes involved in cell adhesion proliferation and the maintenance of tissue structure.
- Pathways
CDX2 is integral to the Wnt signaling and Hedgehog pathways which are vital for intestinal development and homeostasis. Within these pathways CDX2 works closely with other proteins like beta-catenin influencing cell proliferation and differentiation. The Wnt pathway in particular modulates the expression of proliferative genes while CDX2 ensures that proliferation maintains tissue integrity by guiding cellular differentiation.
- Associated diseases and disorders
CDX2 has a significant link to colorectal cancer and Barrett's esophagus. Loss of CDX2 expression often marks the progression from normal to malignant tissue in colorectal cancer where it connects with proteins like p-catenin affecting cellular growth and differentiation. Similarly abnormal expression of CDX2 correlates with the development of Barrett's esophagus a condition that may progress to esophageal adenocarcinoma. These disease associations make CDX2 a potential diagnostic marker and therapeutic target in gastrointestinal pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [CDX2/1690] (ab217884), expandable thumbnail](https://content.abcam.com/products/images/cdx2-antibody-cdx2-1690-ab217884--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130114.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [CDX2/1690] (ab217884)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [CDX2/1690] (ab217884)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling CDX2 with ab217884 at 1 μg/ml.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [CDX2/1690] (ab217884), expandable thumbnail](https://content.abcam.com/products/images/cdx2-antibody-cdx2-1690-ab217884--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62453.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [CDX2/1690] (ab217884)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [CDX2/1690] (ab217884)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling CDX2 with ab217884 at 1 μg/ml.
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