Name: Anti-CDX2 antibody [EPR2764Y]
SKU: ab76541
Rating: 4 (17 reviews)

Anti-CDX2 antibody [EPR2764Y] is a rabbit monoclonal antibody used for detecting CDX2 in western blotting (WB), immunohistochemistry on paraffin-embedded sections (IHC-P), immunocytochemistry/immunofluorescence (ICC/IF), and ChIC/CUT&RUN sequencing. Suitable for human, mouse, and rat samples.

- Cited in over 170 publications
- Recombinant format ensures unrivaled batch-to-batch consistency; no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (AB76541), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF	Flow Cyt (Intra)	ChIC/CUT&RUN-seq
Human	Tested	Tested	Tested	Not recommended	Tested
Mouse	Tested	Expected	Not recommended	Not recommended	Expected
Rat	Tested	Expected	Not recommended	Not recommended	Expected
Rabbit	Predicted	Predicted	Not recommended	Not recommended	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes For unpurified use at 1/250 - 1/500 Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes For unpurified use at 1/250 - 1/500 Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes For unpurified use at 1/250 - 1/500 Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Rabbit	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/10000	Notes For unpurified use at 1/1000 - 1/5000

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rabbit	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/400	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Rabbit	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Rabbit	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 5 µg	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rabbit	Dilution info -	Notes -

Associated Products

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Target data

See full target information CDX2

Function

Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium (By similarity). Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription (By similarity). Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription (By similarity). Binds to and activates transcription of LPH (By similarity). Activates transcription of CLDN2 and intestinal mucin MUC2 (By similarity). Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription (By similarity). Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA (PubMed:28473536).

Alternative names

CDX3, CDX2, Homeobox protein CDX-2, CDX-3, Caudal-type homeobox protein 2

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR2764Y
Purification technique: Affinity purification Protein A
Dissociation constant: 1 x 10^-11 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Stable for 12 months at -20°C, Store in the dark

Notes

Anti-CDX2 antibody [EPR2764Y] (ab76541) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, ICC/IF, IHC-P, WB in human, mouse, rat samples.

Anti-CDX2 antibody [EPR2764Y] (ab76541) has been cited over 134 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality manufacturing and validation processes ensure Anti-CDX2 antibody [EPR2764Y] (ab76541) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-CDX2 antibody [EPR2764Y] (ab76541) has 18 independent reviews from customers.

Anti-CDX2 antibody [EPR2764Y] (ab76541) specifically detects CDX2 (UniProt ID: Q99626; Molecular weight: 34kDa) and is sold in 100 uL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR2764Y - ab22799.

Antibody clone EPR2764Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, PE, APC, HRP, Alkaline Phosphatase, Alexa Fluor® 555, Alexa Fluor® 568 (ab1957, ab1958, ab338, ab339, ab331, ab38964, Alexa Fluor® 555 Anti-CDX2 antibody [EPR2764Y] ab312266, Alexa Fluor® 568 Anti-CDX2 antibody [EPR2764Y] ab312755).

CDX2 is a critical transcription factor involved in intestinal differentiation and is widely used as a prognostic marker in colorectal cancer. Utilizing immunohistochemistry for CDX2 detection can significantly aid in diagnosing and determining the origin of metastatic adenocarcinomas. Research indicates that CDX2 expression is associated with better prognosis and can help guide therapeutic decisions. Validated also for CUT&RUN, which is a key applications to map protein-DNA interactions on a genome-wide scale using NGS.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CDX2 or Caudal Type Homeobox 2 is a transcription factor involved in intestinal development and differentiation. Alternative names for CDX2 include Cdx-2 and CDX-2. This protein has a molecular mass of approximately 38 kDa. CDX2 is expressed mainly in the epithelial cells of the intestine. Researchers can identify CDX2 through techniques like CDX2 stain CDX2 immunostain and CDX2 IHC which are useful for determining its expression in tissue samples.

Biological function summary

CDX2 plays an important role in controlling the expression of genes necessary for maintaining intestinal epithelial cell identity. It functions as a part of regulatory networks that guide cell fate and differentiation in the gut. CDX2 does not generally form complexes but acts as a transcriptional regulator that binds directly to DNA influencing genes involved in cell adhesion proliferation and the maintenance of tissue structure.

Pathways

CDX2 is integral to the Wnt signaling and Hedgehog pathways which are vital for intestinal development and homeostasis. Within these pathways CDX2 works closely with other proteins like beta-catenin influencing cell proliferation and differentiation. The Wnt pathway in particular modulates the expression of proliferative genes while CDX2 ensures that proliferation maintains tissue integrity by guiding cellular differentiation.

Associated diseases and disorders

CDX2 has a significant link to colorectal cancer and Barrett's esophagus. Loss of CDX2 expression often marks the progression from normal to malignant tissue in colorectal cancer where it connects with proteins like p-catenin affecting cellular growth and differentiation. Similarly abnormal expression of CDX2 correlates with the development of Barrett's esophagus a condition that may progress to esophageal adenocarcinoma. These disease associations make CDX2 a potential diagnostic marker and therapeutic target in gastrointestinal pathologies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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19 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Immunohistochemical analysis of formalin fixed paraffin embedded human small intestine labelling CDX2 with ab76541 at a concentration of 0.05 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.
ab76541 anti-CDX2 [EPR2764Y] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)
This image is courtesy of an anonymous customer review
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Unpurified ab76541 staining CDX2 in human intestinal tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hours at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in PBS containing 5% BSA) for 18 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051
was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051
was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-CDX2 antibody [EPR2764Y] (ab76541)
All lanes: Western blot - Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/10000 dilution
Lane 1: Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 2: Human colon cancer lysates at 20 µg
Lane 3: Rat colon lysates at 20 µg
Lane 4: Mouse colon lysates at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/10000 dilution
Predicted band size: 33 kDa
Observed band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Unpurified ab76541, at 1/250 dilution, staining CDX2 in paraffin-embedded human gastric adenocarcinoma tissue, by immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Western blot - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Lane 1: Western blot - Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/1000 dilution
Lanes 2 - 3: Western blot - Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/5000 dilution
Lane 1: HT-29 (Human colorectal adenocarcinoma cell line) lysate at 10 µg
Lane 2: Colon cancer lysate at 10 µg
Lane 3: Human small intestine lysate at 10 µg
Secondary
All lanes: HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Unpurified ab76541 showing positive staining in colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Unpurified ab76541 showing positive staining in normal colon tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Unpurified ab76541 showing negative staining in skeletal muscle tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Unpurified ab76541 showing negative staining in normal kidney tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Unpurified ab76541 showing negative staining in normal brain tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Unpurified ab76541 showing negative staining in normal brain tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
OI-RD Scanning - Anti-CDX2 antibody [EPR2764Y] (ab76541)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [EPR2764Y] (ab76541)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ Caco-2 (human colorectal adenocarcinoma epithelial cell) cells and 5 µg of ab76541 [EPR2764Y]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SW480 ( human colorectal adenocarcinoma epithelial cell) labelling CDX2 with ab76541 at 1/400 (1.82 ?g/ml) dilution (Green).
Negative control: MCF7.
Confocal image showing nuclear staining on the SW480 cell line.
Nuclear DNA was labelled with DAPI (shown in blue).
Secondary used was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ?g/ml).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody -Microtubule Marker (Alexa Fluor® 594) was used as a counterstain at 1/200 (2.5?g/ml) dilution.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [EPR2764Y] (ab76541)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ Caco-2 (human colorectal adenocarcinoma epithelial cell) cells and 5 µg of ab76541 [EPR2764Y]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [EPR2764Y] (ab76541)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ Caco-2 (human colorectal adenocarcinoma epithelial cell) cells and 5 µg of ab76541 [EPR2764Y]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.