Rabbit Recombinant Monoclonal CDX2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra), ChIC/CUT&RUN-seq and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-CDX2 antibody [SP54] - BSA and Azide free (AB240936), expandable thumbnail](https://content.abcam.com/products/images/cdx2-antibody-sp54-bsa-and-azide-free-ab240936--flow-cytometry-intracellular-img139065.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CDX2 antibody [SP54] - BSA and Azide free (AB240936)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [SP54] - BSA and Azide free (AB240936), expandable thumbnail](https://content.abcam.com/products/images/cdx2-antibody-sp54-bsa-and-azide-free-ab240936--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33389.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [SP54] - BSA and Azide free (AB240936)")
![Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [SP54] - BSA and Azide free (AB240936), expandable thumbnail](https://content.abcam.com/products/images/cdx2-antibody-sp54-bsa-and-azide-free-ab240936--immunocytochemistry-immunofluorescence-img76985.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [SP54] - BSA and Azide free (AB240936)")
![ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (AB240936), expandable thumbnail](https://content.abcam.com/products/images/cdx2-antibody-sp54-bsa-and-azide-free-ab240936--chic-cut-run-sequencing-img439400.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (AB240936)")
![ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (AB240936), expandable thumbnail](https://content.abcam.com/products/images/cdx2-antibody-sp54-bsa-and-azide-free-ab240936--chic-cut-run-sequencing-img439399.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (AB240936)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | Flow Cyt (Intra) | ChIC/CUT&RUN-seq | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ChIC/CUT&RUN-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
Target data
Function
Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium (By similarity). Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription (By similarity). Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription (By similarity). Binds to and activates transcription of LPH (By similarity). Activates transcription of CLDN2 and intestinal mucin MUC2 (By similarity). Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription (By similarity). Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA (PubMed:28473536).
Alternative names
CDX3, CDX2, Homeobox protein CDX-2, CDX-3, Caudal-type homeobox protein 2
Recommended products
Rabbit Recombinant Monoclonal CDX2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra), ChIC/CUT&RUN-seq and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- SP54
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240936 is the carrier-free version of Anti-CDX2 antibody [SP54] ab101532.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CDX2 or Caudal Type Homeobox 2 is a transcription factor involved in intestinal development and differentiation. Alternative names for CDX2 include Cdx-2 and CDX-2. This protein has a molecular mass of approximately 38 kDa. CDX2 is expressed mainly in the epithelial cells of the intestine. Researchers can identify CDX2 through techniques like CDX2 stain CDX2 immunostain and CDX2 IHC which are useful for determining its expression in tissue samples.
- Biological function summary
CDX2 plays an important role in controlling the expression of genes necessary for maintaining intestinal epithelial cell identity. It functions as a part of regulatory networks that guide cell fate and differentiation in the gut. CDX2 does not generally form complexes but acts as a transcriptional regulator that binds directly to DNA influencing genes involved in cell adhesion proliferation and the maintenance of tissue structure.
- Pathways
CDX2 is integral to the Wnt signaling and Hedgehog pathways which are vital for intestinal development and homeostasis. Within these pathways CDX2 works closely with other proteins like beta-catenin influencing cell proliferation and differentiation. The Wnt pathway in particular modulates the expression of proliferative genes while CDX2 ensures that proliferation maintains tissue integrity by guiding cellular differentiation.
- Associated diseases and disorders
CDX2 has a significant link to colorectal cancer and Barrett's esophagus. Loss of CDX2 expression often marks the progression from normal to malignant tissue in colorectal cancer where it connects with proteins like p-catenin affecting cellular growth and differentiation. Similarly abnormal expression of CDX2 correlates with the development of Barrett's esophagus a condition that may progress to esophageal adenocarcinoma. These disease associations make CDX2 a potential diagnostic marker and therapeutic target in gastrointestinal pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Flow Cytometry (Intracellular) - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936), expandable thumbnail](https://content.abcam.com/products/images/cdx2-antibody-sp54-bsa-and-azide-free-ab240936--flow-cytometry-intracellular-img139065.jpg "Flow Cytometry (Intracellular) - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)")
Flow Cytometry (Intracellular) - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)
Intracellular Flow Cytometry analysis of Caco-2 (human colorectal adenocarcinoma epithelial cell) labeling CDX2 with purified Anti-CDX2 antibody [SP54] ab101532 at 1/200 dilution (0.56μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Unlableled control -Unlabelled cells (blue).
This image was generated using Anti-CDX2 antibody [SP54] ab101532, the same clone, but with a different buffer formulation.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936), expandable thumbnail](https://content.abcam.com/products/images/cdx2-antibody-sp54-bsa-and-azide-free-ab240936--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33389.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling CDX2 with Anti-CDX2 antibody [SP54] ab101532 at 1/100 dilution (1.11 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-CDX2 antibody [SP54] ab101532 for 30 mins at room temperature.This image was generated using Anti-CDX2 antibody [SP54] ab101532, the same clone, but with a different buffer formulation.
![Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936), expandable thumbnail](https://content.abcam.com/products/images/cdx2-antibody-sp54-bsa-and-azide-free-ab240936--immunocytochemistry-immunofluorescence-img76985.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)")
Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)
Immunocytochemistry/ Immunofluorescence analysis of Caco-2 (human colorectal adenocarcinoma epithelial cell) cells labeling CDX2 with purified Anti-CDX2 antibody [SP54] ab101532 at 1/50 (2.22 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This image was generated using Anti-CDX2 antibody [SP54] ab101532, the same clone, but with a different buffer formulation.
![ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936), expandable thumbnail](https://content.abcam.com/products/images/cdx2-antibody-sp54-bsa-and-azide-free-ab240936--chic-cut-run-sequencing-img439400.jpg "ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)")
ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CDX2 antibody [SP54] ab101532).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 Caco-2 (human colorectal adenocarcinoma epithelial cell) cells and 5 µg of Anti-CDX2 antibody [SP54] ab101532 [SP54]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
![ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936), expandable thumbnail](https://content.abcam.com/products/images/cdx2-antibody-sp54-bsa-and-azide-free-ab240936--chic-cut-run-sequencing-img439399.jpg "ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)")
ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CDX2 antibody [SP54] ab101532).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 Caco-2 (human colorectal adenocarcinoma epithelial cell) cells and 5 µg of Anti-CDX2 antibody [SP54] ab101532 [SP54]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
![ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936), expandable thumbnail](https://content.abcam.com/products/images/cdx2-antibody-sp54-bsa-and-azide-free-ab240936--chic-cut-run-sequencing-img439398.jpg "ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)")
ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CDX2 antibody [SP54] ab101532).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 Caco-2 (human colorectal adenocarcinoma epithelial cell) cells and 5 µg of Anti-CDX2 antibody [SP54] ab101532 [SP54]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
![ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936), expandable thumbnail](https://content.abcam.com/products/images/cdx2-antibody-sp54-bsa-and-azide-free-ab240936--chic-cut-run-sequencing-img439398.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (ab240936)")
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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