Anti-CDX2 antibody [SP54] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CDX2 antibody [SP54] - BSA and Azide free (AB240936)

Intracellular Flow Cytometry analysis of Caco-2 (human colorectal adenocarcinoma epithelial cell) labeling CDX2 with purified ab101532 at 1/200 dilution (0.56μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^®488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (black). Unlableled control -Unlabelled cells (blue).

This image was generated using ab101532, the same clone, but with a different buffer formulation.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [SP54] - BSA and Azide free (AB240936)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling CDX2 with ab101532 at 1/100 dilution (1.11 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101532 for 30 mins at room temperature.

This image was generated using ab101532, the same clone, but with a different buffer formulation.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CDX2 antibody [SP54] - BSA and Azide free (AB240936)

Immunocytochemistry/ Immunofluorescence analysis of Caco-2 (human colorectal adenocarcinoma epithelial cell) cells labeling CDX2 with purified ab101532 at 1/50 (2.22 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This image was generated using ab101532, the same clone, but with a different buffer formulation.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (AB240936)

This data was developed using the same antibody clone in a different buffer formulation (ab101532).

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ Caco-2 (human colorectal adenocarcinoma epithelial cell) cells and 5 µg of ab101532 [SP54]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (AB240936)

This data was developed using the same antibody clone in a different buffer formulation (ab101532).

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ Caco-2 (human colorectal adenocarcinoma epithelial cell) cells and 5 µg of ab101532 [SP54]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-CDX2 antibody [SP54] - BSA and Azide free (AB240936)

This data was developed using the same antibody clone in a different buffer formulation (ab101532).

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ Caco-2 (human colorectal adenocarcinoma epithelial cell) cells and 5 µg of ab101532 [SP54]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.