AB4539

Anti-CEACAM 5 + 6 antibody [MUS]

Name: Anti-CEACAM 5 + 6 antibody [MUS] (ab4539) | Abcam
Brand: Abcam Limited
SKU: AB4539
Price: 520 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal CEACAM5 antibody. Carrier free. Suitable for Flow Cyt, ELISA, WB, IHC-Fr and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human CEACAM5.

View Alternative Names

CD66e, CEA, CEACAM5, Cell adhesion molecule CEACAM5, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, Meconium antigen 100, CEA cell adhesion molecule 5

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MUS

Isotype

IgG1

Light chain type

unknown

Carrier free

Yes

Reacts with

Human

Applications

WB, ELISA, IHC-Fr, Flow Cyt

applications

Immunogen

Native Full Length Protein corresponding to Human CEACAM5.

P06731

Specificity

MUS reacts specifically with CEACAM 5 (CEA/CD66e) and CEACAM 6 (NCA/CD66c) transiently expressed on the cell surface of transfected BOSC23 cells as demonstrated by flow cytometry.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Predicted molecular weight Carcino Embryonic Antigen: 84 kDa. Predicted molecular weight CEACAM 6: 41 kDa", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEACAM5 and CEACAM6 also known as carcinoembryonic antigen-related cell adhesion molecule 5 and 6 are glycoproteins with important mechanical roles in cellular adhesion. CEACAM5 has a molecular weight of approximately 180 kDa and CEACAM6 weighs around 90 kDa. These proteins mainly express in epithelial cells especially in mucosal tissues such as the gastrointestinal tract and respiratory epithelium. CEACAM5 was also identified as the carcinoembryonic antigen (CEA) widely utilized in cancer diagnostics.

Biological function summary

The CEACAM proteins including CEACAM5 and CEACAM6 contribute to cellular adhesion processes and signal transduction. They function as part of larger complexes that mediate cell-cell interactions. By engaging in homophilic and heterophilic binding they help regulate cell proliferation and differentiation. In normal physiological conditions these proteins play roles in maintaining tissue architecture and integrity.

Pathways

CEACAM5 and CEACAM6 proteins involve cellular communication and immune system pathways. They interact with signaling molecules part of pathways like the MAPK and PI3K-Akt pathways influencing cellular responses to external stimuli. Related proteins such as CEACAM1 can modulate immune reactions and cell signaling showing the interconnected roles of CEACAM family members in these pathways.

CEACAM5 and CEACAM6 connect strongly with various cancers particularly colorectal and lung cancer. Overexpression of these proteins often indicates tumor progression and metastasis. CEACAM5 is frequently used as a marker for colorectal cancer prognosis while CEACAM6 is associated with the invasive nature of cancer cells. Both proteins interact with pathways involving epidermal growth factor receptor (EGFR) highlighting their relevance in oncogenic processes and potential as therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed : 10864933, PubMed : 10910050, PubMed : 2803308). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed : 2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed : 10910050).. (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.

See full target information CEACAM5

Additional targets

CEACAM6

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 89:104476 PubMed36801616

2023

NIR-II fluorescence imaging-guided colorectal cancer surgery targeting CEACAM5 by a nanobody.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyong Guo,Changjian Li,Xiaohua Jia,Yawei Qu,Miaomiao Li,Caiguang Cao,Zeyu Zhang,Qiaojun Qu,Shuangling Luo,Jianqiang Tang,Haifeng Liu,Zhenhua Hu,Jie Tian

Experimental eye research 150:44-61 PubMed26220834

2015

Idiopathic preretinal glia in aging and age-related macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Malia M Edwards,D Scott McLeod,Imran A Bhutto,Mercedes B Villalonga,Johanna M Seddon,Gerard A Lutty

Clinical cancer research : an official journal of the American Association for Cancer Research 12:2976-87 PubMed16707592

2006

RNA interference suppression of MUC1 reduces the growth rate and metastatic phenotype of human pancreatic cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hideaki Tsutsumida,Benjamin J Swanson,Pankaj K Singh,Thomas C Caffrey,Shinichi Kitajima,Masamichi Goto,Suguru Yonezawa,Michael A Hollingsworth

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CEACAM 5 + 6 antibody [MUS]

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (3)

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