Anti-CEACAM1 antibody [EPR26490-88]
- BOND RX™ Validated
- 20ul selling size
- RabMAb
- Recombinant
- KO Validated
- What is this?
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(1 Publication)
Rabbit Recombinant Monoclonal CEAM1 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt, IP and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication.
View Alternative Names
CD66a, BGP, BGP1, CEACAM1, Cell adhesion molecule CEACAM1, Biliary glycoprotein 1, Carcinoembryonic antigen-related cell adhesion molecule 1, BGP-1, CEA cell adhesion molecule 1
- ICC
Supplier Data
Immunocytochemistry - Anti-CEACAM1 antibody [EPR26490-88] (AB300061)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized CEACAM1 KO A549 (CEACAM1 knockout human lung carcinoma epithelial cell) (ab267047) cells lebelling CEACAM1 with ab300061 at 1/500 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing membrane and cytoplasmic staining in parental A549 cells, no staining in CEACAM1 KO A549 cells.
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
- Flow Cyt
Supplier Data
Flow Cytometry - Anti-CEACAM1 antibody [EPR26490-88] (AB300061)
Flow cytometric analysis of Wild-type A549 (Human lung carcinoma epithelial cell, Right)/ CEACAM1 knockout A549 cells (Left) cells labelling CEACAM1 with ab300061 at 1/500 dilution/ Left and Right compared with a isotype control. Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Positive staining on A549 cells (ab255450), while no staining on CEACAM1 knockout A549 cells (ab267047).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR26490-88] (AB300061)
Immunohistochemical analysis of paraffin-embedded human colon tissue labelling CEACAM1 with ab300061 at 1/2000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon (PMID : 8543780). The section was incubated with ab300061 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : PBS was used instead of primary antibody (ab300061) followed by ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR26490-88] (AB300061)
Immunohistochemical analysis of paraffin-embedded human liver tissue labelling CEACAM1 with ab300061 at 1/2000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver (PMID : 27377843). The section was incubated with ab300061 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : PBS was used instead of primary antibody (ab300061) followed by ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR26490-88] (AB300061)
Immunohistochemical analysis of paraffin-embedded human endometrium tissue labelling CEACAM1 with ab300061 at 1/2000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human endometrium (PMID : 9626043). The section was incubated with ab300061 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : PBS was used instead of primary antibody (ab300061) followed by ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR26490-88] (AB300061)
Immunohistochemical analysis of paraffin-embedded human hepatic carcinoma tissue labelling CEACAM1 with ab300061 at 1/2000 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human hepatic carcinoma (PMID : 27377843). The section was incubated with ab300061 for 30 mins at room temperature The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : PBS was used instead of primary antibody (ab300061) followed by ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- IP
Supplier Data
Immunoprecipitation - Anti-CEACAM1 antibody [EPR26490-88] (AB300061)
CEACAM1 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate 10 µg with ab300061 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab300061 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : A549 (human lung carcinoma epithelial cell) whole cell lysate 10 µg (Input). Lane 2 : ab300061 IP in A549 whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300061 in A549 whole cell lysate. Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 32 seconds
All lanes:
Immunoprecipitation - Anti-CEACAM1 antibody [EPR26490-88] (ab300061) at 1/30 dilution
Lane 1:
A549 (human lung carcinoma epithelial cell) whole cell lysate at 10 µg
Lane 2:
ab300061 IP in A549 whole cell lysate
Lane 3:
Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab300061 in A549 whole cell lysate
false
- WB
Supplier Data
Western blot - Anti-CEACAM1 antibody [EPR26490-88] (AB300061)
Lanes 1-3 : Merged signal (red and green). Green - ab300061 observed at 100-180 kDa. Red - loading control ab8245 observed at 36 kDa. ab300061 Anti-CEACAM1 antibody [EPR26490-88] was shown to specifically react with CEACAM1 in wild-type A549 cells (ab255450). Loss of signal was observed when knockout cell line (ab267047) was used. Wild-type and CEACAM1 knockout samples were subjected to SDS-PAGE. ab300061 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4°C overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-CEACAM1 antibody [EPR26490-88] (ab300061) at 1/1000 dilution
Lane 1:
Wild-type A549 (ab255450) whole cell lysate at 20 µg
Lane 2:
Western blot - Human CEACAM1 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-ceacam1-knockout-a549-cell-line-ab267047'>ab267047</a>) at 20 µg
Lane 3:
HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/10000 dilution
Observed band size: 100 kDa,180 kDa
false
- WB
Supplier Data
Western blot - Anti-CEACAM1 antibody [EPR26490-88] (AB300061)
Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : Lanes 1-3 : 10 seconds; Lane 4 : 48 seconds.
All lanes:
Western blot - Anti-CEACAM1 antibody [EPR26490-88] (ab300061) at 1/1000 dilution
Lane 1:
Human kidney tissue lysate at 20 µg
Lane 2:
Human colon tissue lysate at 20 µg
Lane 3:
A-204 (Human muscle rhabdomyosarcoma) whole cell lysate at 20 µg
Lane 4:
SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Lanes 1 - 4:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Lanes 1 - 4:
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/2000 dilution
false
- WB
Supplier Data
Western blot - Anti-CEACAM1 antibody [EPR26490-88] (AB300061)
Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : Lanes 1-3 : 10 seconds; Lane 4 : 48 seconds.
All lanes:
Western blot - Anti-CEACAM1 antibody [EPR26490-88] (ab300061) at 1/1000 dilution
Lane 1:
His-tagged Human CEACAM-1 recombinant protein aa (aa35-428) at 20 ng
Lane 2:
His-tagged Human CEACAM-5/CD66e recombinant protein (aa35-685) at 20 ng
Lane 3:
His-tagged Human CEACAM-6/CD66c recombinant protein (aa35-320) at 20 ng
Lane 4:
His-tagged Human CEACAM-8/CD66b recombinant protein(aa35-320) at 20 ng
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 100 kDa,180 kDa
false
Exposure time: 48s
Related conjugates and formulations (2)
-
Anti-CEACAM1 antibody [EPR26490-88] (BSA and Azide free)
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-CEACAM1 antibody [EPR26490-88]
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CEACAM1 influences cellular adhesion and immune responses by mediating cell-cell interactions. It forms homophilic dimers at the cell surface and it can participate in heterophilic interactions with other proteins making it part of complex cell signaling networks. These interactions regulate immune cell functions contributing to innate immune responses and cellular homeostasis. CEACAM1 also modulates angiogenesis endothelial cell permeability and apoptosis.
Pathways
CEACAM1 is involved in signaling and regulatory pathways important for immune surveillance and inflammation. It is a part of the TGF-beta signaling pathway affecting cellular proliferation and differentiation. CEACAM1 interacts with proteins such as SHP-1 and SHP-2 which are essential in modulating immune responses. Additionally CEACAM1 influences the MAPK/ERK pathway which plays a role in cell growth and survival.
Product protocols
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Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Frontiers in immunology 16:1522346 PubMed40046057
2025
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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