Anti-CEACAM1 antibody [EPR4048] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR4048] - BSA and Azide free (AB179460)

ab108390, at 1/100 dilution, staining CEACAM1 in Human liver tissue by immunohistochemistry.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108390).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR4048] - BSA and Azide free (AB179460)

ab108390, at 1/100 dilution, staining CEACAM1 in Human kidney tissue by immunohistochemistry.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108390).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR4048] - BSA and Azide free (AB179460)

Immunohistochemical analysis of paraffin-embedded sections (A) HEK-293T cells transfected with a CEACAM1 expression vector containing a his tag. (B) HEK-293T cells transfected with a CEACAM3 expression vector containing a his tag. (C) HEK-293T cells transfected with a CEACAM5 expression vector containing a his tag. (D) HEK-293T cells transfected with a CEACAM6 expression vector containing a his tag. (E) HEK-293T cells transfected with a CEACAM8 expression vector containing a his tag. (F) HEK-293T cells transfected with empty vector containing a his tag. This was labelling CEACAM1 with ab108390 at 1/2000 dilution. The section was incubated with ab108390 for 30 mins at room temperature. LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Sections were counter stained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Positive staining on (A) HEK-293T cells transfected with a CEACAM1 expression vector containing a his tag, (B) HEK-293T cells transfected with a CEACAM3 expression vector containing a his tag and (C) HEK-293T cells transfected with a CEACAM5 expression vector containing a his tag. No staining on (D) HEK-293T cells transfected with a CEACAM6 expression vector containing a his tag, (E) HEK-293T cells transfected with a CEACAM8 expression vector containing a his tag and (F) HEK-293T cells transfected with empty vector containing a his tag. The results indicated cross reaction with CEACAM1, CEACAM3 and CEACAM5. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. This data was developed using the same antibody clone in a different buffer formulation (ab108390).

• WB

Lab

Western blot - Anti-CEACAM1 antibody [EPR4048] - BSA and Azide free (AB179460)

This data was developed using the same antibody clone in a different buffer formulation (ab108390).

Lanes 1 - 2 : Merged signal (red and green). Green - ab108390 observed at 125 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab108390 was shown to react with KDM5C / Jarid1C / SMCX in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab108390 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CEACAM1 antibody [EPR4048] (<a href='/en-us/products/primary-antibodies/ceacam1-antibody-epr4048-ab108390'>ab108390</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HEK-293 cell lysate at 20 µg

Predicted band size: 57 kDa

Observed band size: 45 kDa

false

• WB

Lab

Western blot - Anti-CEACAM1 antibody [EPR4048] - BSA and Azide free (AB179460)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST This antibody cross-reacts with CEAM-3 and CEAM-5. This data was developed using the same antibody clone in a different buffer formulation (ab108390).

All lanes:

Western blot - Anti-CEACAM1 antibody [EPR4048] (<a href='/en-us/products/primary-antibodies/ceacam1-antibody-epr4048-ab108390'>ab108390</a>) at 1/1000 dilution

Lane 1:

His-tagged human CEAM-1 fragment at 10 ng

Lane 2:

His-tagged human CEAM-3 fragment at 10 ng

Lane 3:

His-tagged human CEAM-5 fragment at 10 ng

Lane 4:

His-tagged human CEAM-6 fragment at 10 ng

Lane 5:

His-tagged human CEAM-8 fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 57 kDa

Observed band size: 180 kDa,25 kDa,66 kDa

false

Exposure time: 37s