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Rabbit Recombinant Monoclonal CEAM1 antibody. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (AB206431), expandable thumbnail
  • Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (AB206431), expandable thumbnail
  • Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (AB206431), expandable thumbnail
  • Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (AB206431), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (AB206431), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PFlow Cyt
Human
Tested
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Target data

Function

Isoform 1Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner (By similarity). Plays a role as coinhibitory receptor in immune response, insulin action and functions also as an activator during angiogenesis (PubMed:18424730, PubMed:23696226, PubMed:25363763). Its coinhibitory receptor function is phosphorylation- and PTPN6 -dependent, which in turn, suppress signal transduction of associated receptors by dephosphorylation of their downstream effectors. Plays a role in immune response, of T cells, natural killer (NK) and neutrophils (PubMed:18424730, PubMed:23696226). Upon TCR/CD3 complex stimulation, inhibits TCR-mediated cytotoxicity by blocking granule exocytosis by mediating homophilic binding to adjacent cells, allowing interaction with and phosphorylation by LCK and interaction with the TCR/CD3 complex which recruits PTPN6 resulting in dephosphorylation of CD247 and ZAP70 (PubMed:18424730). Also inhibits T cell proliferation and cytokine production through inhibition of JNK cascade and plays a crucial role in regulating autoimmunity and anti-tumor immunity by inhibiting T cell through its interaction with HAVCR2 (PubMed:25363763). Upon natural killer (NK) cells activation, inhibit KLRK1-mediated cytolysis of CEACAM1-bearing tumor cells by trans-homophilic interactions with CEACAM1 on the target cell and lead to cis-interaction between CEACAM1 and KLRK1, allowing PTPN6 recruitment and then VAV1 dephosphorylation (PubMed:23696226). Upon neutrophils activation negatively regulates IL1B production by recruiting PTPN6 to a SYK-TLR4-CEACAM1 complex, that dephosphorylates SYK, reducing the production of reactive oxygen species (ROS) and lysosome disruption, which in turn, reduces the activity of the inflammasome. Downregulates neutrophil production by acting as a coinhibitory receptor for CSF3R by downregulating the CSF3R-STAT3 pathway through recruitment of PTPN6 that dephosphorylates CSF3R (By similarity). Also regulates insulin action by promoting INS clearance and regulating lipogenesis in liver through regulating insulin signaling (By similarity). Upon INS stimulation, undergoes phosphorylation by INSR leading to INS clearance by increasing receptor-mediated insulin endocytosis. This inernalization promotes interaction with FASN leading to receptor-mediated insulin degradation and to reduction of FASN activity leading to negative regulation of fatty acid synthesis. INSR-mediated phosphorylation also provokes a down-regulation of cell proliferation through SHC1 interaction resulting in decrease coupling of SHC1 to the MAPK3/ERK1-MAPK1/ERK2 and phosphatidylinositol 3-kinase pathways (By similarity). Functions as activator in angiogenesis by promoting blood vessel remodeling through endothelial cell differentiation and migration and in arteriogenesis by increasing the number of collateral arteries and collateral vessel calibers after ischemia. Also regulates vascular permeability through the VEGFR2 signaling pathway resulting in control of nitric oxide production (By similarity). Downregulates cell growth in response to EGF through its interaction with SHC1 that mediates interaction with EGFR resulting in decrease coupling of SHC1 to the MAPK3/ERK1-MAPK1/ERK2 pathway (By similarity). Negatively regulates platelet aggregation by decreasing platelet adhesion on type I collagen through the GPVI-FcRgamma complex (By similarity). Inhibits cell migration and cell scattering through interaction with FLNA; interfers with the interaction of FLNA with RALA (PubMed:16291724). Mediates bile acid transport activity in a phosphorylation dependent manner (By similarity). Negatively regulates osteoclastogenesis (By similarity).Isoform 8Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner (By similarity). Promotes populations of T cells regulating IgA production and secretion associated with control of the commensal microbiota and resistance to enteropathogens (By similarity).

Targets

CEACAM6

Alternative names

Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein 1, BGP-1, BGP, BGP1, CEACAM1

Recommended products

Rabbit Recombinant Monoclonal CEAM1 antibody. Suitable for WB, IHC-P and reacts with Human samples.

Alternative names

Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein 1, BGP-1, BGP, BGP1, CEACAM1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR19878

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Carcinoembryonic antigen-related cell adhesion molecules 1 and 6 (CEACAM1 and CEACAM6) are members of the immunoglobulin superfamily. CEACAM1 known also as CD66a or BGP has a mass of approximately 110 kDa. CEACAM6 also referred to as CD66c or NCA is around 37 kDa. Both proteins are broadly expressed on epithelial cells in tissues like the colon and lung. CEACAM1 shows higher diversity in isoforms due to alternative splicing allowing it to modulate diverse functions depending on its form. CEACAM6 expression is typically found on cells of the gastrointestinal tract and in granulocytes.

Biological function summary

CEACAM1 and CEACAM6 act as cell adhesion molecules. These proteins help mediate intercellular adhesion and communication which plays a role in maintaining tissue organization and integrity. CEACAM1 is part of complexes involved in intracellular signaling influencing processes like cell proliferation and apoptosis. CEACAM6 lacks significant intracellular signaling domains but contributes to pathogen binding and immune response modulation. Their ability to bind with other CEACAM family members helps regulate cell-cell and cell-matrix interactions.

Pathways

The CEACAM1 and CEACAM6 proteins play roles in key signaling pathways such as the MAPK/ERK and PI3K/AKT pathways. CEACAM1 links to the MAPK/ERK pathway through its ITIM domain modulating cell growth and survival. CEACAM6 interacts with various kinases influencing the PI3K/AKT pathway aiding in cell migration and evasion of apoptosis. Together these proteins participate in pathways that coordinate cell adhesion and signal transduction sharing connections with proteins like beta-catenin.

Associated diseases and disorders

CEACAM1 and CEACAM6 have strong associations with cancer development especially colorectal and lung cancers. CEACAM1 plays a role in tumor progression by affecting immune cell function and vascular growth. Its downregulation often links with the tumor’s ability to evade the immune system. CEACAM6 overexpression correlates with tumor invasiveness and poor prognosis impacting its connection to effector proteins such as integrins. Both proteins present potential targets for therapeutic strategies due to their involvement in cancer-related mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431), expandable thumbnail

    Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human CEACAM1 recombinant protein contains aa35-428 with GST-tag and His-Tag®. Human CEACAM5, CEACAM6, CEACAM7, CEACAM8 recombinant proteins contain aa35-422, aa35-326, aa35-326 and aa35-335 respectively, and all with a His-Tag®.

    All lanes: Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431) at 1/1000 dilution

    Lane 1: Human CEACAM1 recombinant protein at 0.01 µg

    Lane 2: Human CEACAM5 recombinant protein at 0.01 µg

    Lane 3: Human CEACAM6 recombinant protein at 0.01 µg

    Lane 4: Human CEACAM7 recombinant protein at 0.01 µg

    Lane 5: Human CEACAM8 recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 57 kDa

    Exposure time: 1s

    This data was developed using ab206431, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Human CEACAM1 recombinant protein contains aa35-428 with GST-tag and His-Tag®. Human CEACAM5, CEACAM6, CEACAM7, CEACAM8 recombinant proteins contain aa35-422, aa35-326, aa35-326 and aa35-335 respectively, and all with a His-Tag®.

  • Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431), expandable thumbnail

    Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second; Lane 2: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 19244123, PMID: 10751340, PMID: 17081782).

    All lanes: Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431) at 1/1000 dilution

    Lane 1: BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate at 10 µg

    Lane 2: Human kidney tissue lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 57 kDa

    Observed band size: 110-150 kDa, 75-90 kDa

    This data was developed using ab206431, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 1 second; Lane 2: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 19244123, 10751340, 17081782).

  • Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431), expandable thumbnail

    Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 minute; Lane 2: 3 seconds; Lane 3: 30 seconds; Lane 4: 15 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 19244123, PMID: 10751340, PMID: 17081782).

    All lanes: Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431) at 1/1000 dilution

    Lane 1: Human fetal liver tissue lysate at 10 µg

    Lane 2: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

    Lane 3: A549 (Human lung carcinoma cell line) whole cell lysate at 10 µg

    Lane 4: HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 57 kDa

    Observed band size: 110-150 kDa

    This data was developed using ab206431, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 1 minute; Lane 2: 3 seconds; Lane 3: 30 seconds; Lane 4: 15 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 19244123; 10751340; 17081782).

  • Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431), expandable thumbnail

    Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    HUVEC does not express CEACAM 6 and PC-3 does not express CEACAM 1 (PMID: 22918079, PMID: 10359532).

    All lanes: Western blot - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431) at 1/1000 dilution

    Lane 1: HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 10 µg

    Lane 2: PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 16 kDa, 57 kDa

    Observed band size: 13 kDa

    Exposure time: 3min

    This data was developed using ab206431, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    HUVEC does not express CEACAM 6 and PC-3 does not express CEACAM 1 (PMID: 22918079, 10359532).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CEACAM1 + CEACAM6 with ab206431 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Brush border staining on human colon is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 + CEACAM6 antibody [EPR19878] (ab206431)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling CEACAM1 + CEACAM6 with ab206431 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Membrane staining on human liver cancer is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

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