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AB59626

Anti-CEACAM20 antibody [HT-12D8]

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Mouse Monoclonal CEACAM20 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Transfected cell line samples.

View Alternative Names

UNQ9366/PRO34155, CEACAM20, Cell adhesion molecule CEACAM20, Carcinoembryonic antigen-related cell adhesion molecule 20, CEA cell adhesion molecule 20

2 Images
Flow Cytometry - Anti-CEACAM20 antibody [HT-12D8] (AB59626)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CEACAM20 antibody [HT-12D8] (AB59626)

BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6 : D14HD11; CEACAM7 : BAC2; CEACAM8 : Tet2; CEACAM19,21 : a-myc; CEACAM20 : a-flag; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified HT-12D8 (ab59626) was tested on all CEACAM transfectants. A positive signal was obtained onlywith CEACAM20 transfected cells (red curve).

Immunocytochemistry/ Immunofluorescence - Anti-CEACAM20 antibody [HT-12D8] (AB59626)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CEACAM20 antibody [HT-12D8] (AB59626)

Spectral Confocal Microscopy of CHO cells using ab59626 at 1ug/106 cells. CHO cells were transiently transfected with an expression vector encoding CEACAM20. Binding of HT-12D8 (ab59626) was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HT-12D8

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab59626 does not react with CEACAM1, CEACAM3-8 or CEACAM19-21.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1.2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/cells for 6 µg/cells", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab59626 was purified by protein G affinity chromatography from cell culture supernatants.
Storage buffer
pH: 7.2 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEACAM20 also known as carcinoembryonic antigen-related cell adhesion molecule 20 is a member of the CEACAM family of proteins. It has a molecular weight of approximately 32 kDa. This protein is expressed mainly in epithelial tissues including in the gastrointestinal tract and lungs. Its expression is often context-dependent appearing in specific tissues during particular developmental stages or conditions. CEACAM proteins in general play roles in cell signaling and cell adhesion.
Biological function summary

CEACAM20 contributes to processes such as cell-cell communication and immune response modulation. It may form part of a larger complex with other CEACAM proteins engaging in homo- or heterophilic interactions which help mediate its role in cellular adhesion. These interactions can affect immune cell activation and the epithelial tissue integrity where it is expressed hinting at its involvement in maintaining tissue function under various physiological conditions.

Pathways

CEACAM20 plays roles in the immune response and epithelial cell signaling pathways. It interacts closely with other CEACAM family proteins such as CEACAM1 and CEACAM8 which are involved in similar pathways. These interactions likely influence the recruitment and activation of immune cells. Through these pathways CEACAM20 helps with communication between cells in the immune system and epithelial layers aiding in effective response mechanisms and barrier protection.

Increased or altered expression of CEACAM20 has been linked to inflammatory diseases and cancer. In inflammatory bowel disease its expression can impact epithelial function and immune response potentially exacerbating the condition. In cancer particularly colorectal cancer CEACAM20's interaction with proteins such as CEACAM1 could influence tumor growth and immune evasion. Understanding these interactions may provide insight into potential therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Together with the tyrosine-protein kinase SYK, enhances production of the cytokine CXCL8/IL-8 via the NFKB pathway and may thus have a role in the intestinal immune response.
See full target information CEACAM20
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Product promise

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