AB196606

Anti-CEACAM3 antibody

Name: Anti-CEACAM3 antibody (ab196606) | Abcam
Brand: Abcam Limited
SKU: AB196606
Price: 520 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal CEAM3 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CEACAM3.

View Alternative Names

CD66d, CD66D, CGM1, CEACAM3, Cell adhesion molecule CEACAM3, Carcinoembryonic antigen CGM1, Carcinoembryonic antigen-related cell adhesion molecule 3, CEA cell adhesion molecule 3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM3 antibody (AB196606)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM3 antibody (AB196606)

Immunohistochemical analysis of paraffin-embedded human epididymis labeling CEACAM3 using ab196606 at 1/100 dilution

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM3 antibody (AB196606)

Immunohistochemical analysis of paraffin-embedded human lung labeling CEACAM3 using ab196606 at 1/100 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM3 antibody (AB196606)

Immunohistochemical analysis of paraffin-embedded human testis labeling CEACAM3 using ab196606 at 1/100 dilution.

Supplier Data

Western blot - Anti-CEACAM3 antibody (AB196606)

All lanes:

Western blot - Anti-CEACAM3 antibody (ab196606) at 1/500 dilution

Lane 1:

A549 cell lysate

Lane 2:

HT29 cell lysate

Lane 3:

Mouse liver lysate

Lane 4:

Mouse lung lysate

Lane 5:

Mouse testis lysate

Predicted band size: 27 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CEACAM3. The exact immunogen used to generate this antibody is proprietary information.

P40198

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEACAM3 also known as CD66d is a membrane protein involved in cell adhesion and immune response. It weighs approximately 29 kDa and belongs to the carcinoembryonic antigen (CEA) family. CEACAM3 primarily expresses on granulocytes especially neutrophils. It plays a role in cellular interactions contributing to the body's defense against certain pathogens.

Biological function summary

CEACAM3 facilitates immune surveillance by enabling neutrophils to recognize and engulf bacteria through opsonin-independent phagocytosis. It interacts with the actin cytoskeleton dynamics although it does not form a larger protein complex. CEACAM3 binds to specific bacterial adhesins helping initiate a rapid immune response which is essential in eliminating pathogens at sites of infection.

Pathways

CEACAM3 is involved in the regulation of phagocytosis and inflammation. It activates a signaling cascade that includes proteins such as Src-family kinases and small GTPases like Rac and Cdc42 which are important for actin reorganization and engulfment of bacteria. These pathways support cellular processes essential for neutrophil-mediated clearance of infectious agents enhancing the body's ability to control bacterial infections.

Research implicates CEACAM3 in certain bacterial infections and inflammatory conditions. Dysregulation or functional impairment can lead to chronic inflammation or excessive tissue damage. CEACAM1 a related protein can modulate immune responses alongside CEACAM3 suggesting potential overlaps in their functions and impacts in these disease contexts. Understanding CEACAM3's role can help in developing therapies that target these pathways to treat bacterial infections and manage inflammation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neissiria, Moxarella and Haemophilus species, thus playing an important role in the clearance of pathogens by the innate immune system. Responsible for RAC1 stimulation in the course of pathogen phagocytosis.

See full target information CEACAM3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:3813 PubMed38714682

2024

Replication competent HIV-guided CRISPR screen identifies antiviral factors including targets of the accessory protein Nef.

Applications

Unspecified application

Species

Unspecified reactive species

Caterina Prelli Bozzo,Alexandre Laliberté,Aurora De Luna,Chiara Pastorio,Kerstin Regensburger,Stefan Krebs,Alexander Graf,Helmut Blum,Meta Volcic,Konstantin M J Sparrer,Frank Kirchhoff

Cancer genomics & proteomics 20:30-39 PubMed36581340

2022

Rab27b, a Regulator of Exosome Secretion, Is Associated With Peritoneal Metastases in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sho Nambara,Takaaki Masuda,Kosuke Hirose,Qingjiang Hu,Taro Tobo,Yuki Ozato,Junji Kurashige,Yoshiki Hiraki,Yuichi Hisamatsu,Tomohiro Iguchi,Keishi Sugimachi,Eiji Oki,Tomoharu Yoshizumi,Koshi Mimori

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CEACAM3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM3 antibody (AB196606)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM3 antibody (AB196606)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM3 antibody (AB196606)

Western blot - Anti-CEACAM3 antibody (AB196606)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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