Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055) is a rabbit monoclonal antibody that is used to detect CEACAM5 in Western Blot, IP, IHC-P. Suitable for Human samples.
- BSA free for reduced non-specific background staining
-Recombinant format for unrivalled batch-batch consistency
Images
![Western blot - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055), expandable thumbnail](https://content.abcam.com/products/images/ceacam5-antibody-blr198j-bsa-free-ab314055--western-blot-img420417.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)")
![Immunoprecipitation - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055), expandable thumbnail](https://content.abcam.com/products/images/ceacam5-antibody-blr198j-bsa-free-ab314055--immunoprecipitation-img420418.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)")
![Immunocytochemistry - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055), expandable thumbnail](https://content.abcam.com/products/images/ceacam5-antibody-blr198j-bsa-free-ab314055--immunocytochemistry-img420422.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)")
![Immunocytochemistry - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055), expandable thumbnail](https://content.abcam.com/products/images/ceacam5-antibody-blr198j-bsa-free-ab314055--immunocytochemistry-img420421.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055), expandable thumbnail](https://content.abcam.com/products/images/ceacam5-antibody-blr198j-bsa-free-ab314055--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420420.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human CEACAM5 aa 1-700. The exact immunogen used to generate this antibody is proprietary information. Database link P06731
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Reactivity data
Select an application| ICC | IHC-P | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Target data
Function
Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:10864933, PubMed:10910050, PubMed:2803308). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed:10910050). (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.
Alternative names
CD66e, CEA, CEACAM5, Cell adhesion molecule CEACAM5, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, Meconium antigen 100, CEA cell adhesion molecule 5
Recommended products
Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055) is a rabbit monoclonal antibody that is used to detect CEACAM5 in Western Blot, IP, IHC-P. Suitable for Human samples.
- BSA free for reduced non-specific background staining
-Recombinant format for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human CEACAM5 aa 1-700. The exact immunogen used to generate this antibody is proprietary information. Database link P06731
- Clone number
- BLR198J
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), in Human samples.
What is the molecular weight of CEACAM5?
Anti-CEACAM5 [BLR198J] - BSA free (ab314055) specifically detects a band for CEACAM5 (UniProt: P06731) at a molecular weight of 76kDa.
Recommended positive controls
WB: Human KM12, MCF-7, HEK293T, TT, and HT-29 cells.IP: TT cellsIHC: Human Bladder carcinoma, Human liver carcinomaICC: Human colon carcinoma, human CEACAM1, CEACAM3, CEACAM5 and CEACAM6 cells
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CEACAM5 also known as Carcinoembryonic Antigen (CEA) or CD66e is a glycoprotein found mainly on the surface of epithelial cells. This protein has a mass of approximately 180 kDa. It gets expressed in a variety of tissues with high expression in the gastrointestinal tract including the colon and rectum. CEACAM5 is a member of the immunoglobulin superfamily and plays a role in cell adhesion influencing how cells stick to each other.
- Biological function summary
CEACAM5 contributes to cellular processes involving cell adhesion and immune response modulation. This protein operates as part of a larger group of cell adhesion molecules and does not function as part of a complex. CEACAM5 can affect the interactions between cells and the extracellular matrix which has implications for tissue structuring and integrity. It helps the immune system recognize and respond to infectious agents or cellular anomalies.
- Pathways
CEACAM5 involvement extends to immune response regulation and cell adhesion pathways. It interacts functionally with proteins like E-cadherin in these pathways which contribute to maintaining epithelial integrity. These pathways are critical for normal tissue maintenance and immune system function especially in mucosal surfaces where pathogen entry occurs. Understanding CEACAM5 interactions provides insight into cellular communication and adhesion mechanisms.
- Associated diseases and disorders
CEACAM5 is associated with colorectal cancer and lung adenocarcinoma. Its overexpression and release into the bloodstream make it a popular biomarker for these diseases. CEACAM5 is used in clinical applications such as CEA ELISA assays for cancer detection and monitoring. In colorectal cancer CEACAM5 interacts with proteins like CEACAM6 to promote tumor invasiveness and metastasis. Anti-CEACAM5 therapeutics including antibody-drug conjugates (anti-CEACAM5 ADC) are being developed to target and treat associated malignancies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
![Western blot - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055), expandable thumbnail](https://content.abcam.com/products/images/ceacam5-antibody-blr198j-bsa-free-ab314055--western-blot-img420417.jpg "Western blot - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055)")
Western blot - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055)
Samples: Whole cell lysate (50 µg) from KM12 (10 µg), MCF-7, HEK293T, TT (2 µg), and HT-29 cells. Antibody:Rabbit anti-CEACAM5 recombinant monoclonal antibody [BLR198J] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody
All lanes: Western blot - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055) at 1/1000 dilution
Lane 1: KM12 Whole cell lysate at 10 µg
Lane 2: MCF-7 Whole cell lysate at 50 µg
Lane 3: HEK-293T Whole cell lysate at 50 µg
Lane 4: TT Whole cell lysate at 2 µg
Lane 5: HT-29 Whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
![Immunoprecipitation - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055), expandable thumbnail](https://content.abcam.com/products/images/ceacam5-antibody-blr198j-bsa-free-ab314055--immunoprecipitation-img420418.jpg "Immunoprecipitation - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055)")
Immunoprecipitation - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055)
Samples:Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from TT cells. Antibodies: Rabbit anti-CEACAM5 recombinant monoclonal antibody [BLR198J] used for IP at 20 µl/mg lysate. CEACAM5 was also immunoprecipitated by a second antibody against a different epitope of CEACAM5. For blotting immunoprecipitated CEACAM5, A700-198 was used at 1:1000. Chemiluminescence with an exposure time of 75 seconds.
![Immunocytochemistry - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055), expandable thumbnail](https://content.abcam.com/products/images/ceacam5-antibody-blr198j-bsa-free-ab314055--immunocytochemistry-img420422.jpg "Immunocytochemistry - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055)")
Immunocytochemistry - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055)
Detection of human CEACAM5 by immunocytochemistry Sample:FFPE section of over-expressing CEACAM1, CEACAM3, CEACAM5 and CEACAM6 cells. Antibody: Rabbit anti-CEACAM5 recombinant monoclonal antibody [BLR198J]. Secondary:HRP-conjugated goat anti-rabbit IgG
![Immunocytochemistry - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055), expandable thumbnail](https://content.abcam.com/products/images/ceacam5-antibody-blr198j-bsa-free-ab314055--immunocytochemistry-img420421.jpg "Immunocytochemistry - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055)")
Immunocytochemistry - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055)
Detection of human CEACAM5 by immunocytochemistry Sample:FFPE section of KM12 (colon carcinoma) cells. Antibody:Rabbit anti-CEACAM5 recombinant monoclonal antibody [BLR198J]. Secondary:HRPconjugated goat anti-rabbit IgG
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055), expandable thumbnail](https://content.abcam.com/products/images/ceacam5-antibody-blr198j-bsa-free-ab314055--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420420.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055)
Sample:FFPE section of bladder carcinoma. Antibody: Rabbit anti-CEACAM5 recombinant monoclonal antibody [BLR198J] (A700-198 lot 1). Secondary:HRP-conjugated goat anti-rabbit IgG (A120-501P).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055), expandable thumbnail](https://content.abcam.com/products/images/ceacam5-antibody-blr198j-bsa-free-ab314055--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420419.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055)
Sample:FFPE sections of liver carcinoma. Antibody:Rabbit anti-CEACAM5 recombinant monoclonal antibody [BLR198J] (A700-198 lot 1) (left -negative) and Rabbit anti-CEACAM1 recombinant monoclonal antibody [BLR090G] (A700-090-1) (right -positive). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055), expandable thumbnail](https://content.abcam.com/products/images/ceacam5-antibody-blr198j-bsa-free-ab314055--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420419.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055)")
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