AB314055

Anti-CEACAM5 antibody [BLR198J] - BSA free

Recombinant
What is this?

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Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055) is a rabbit monoclonal antibody detecting CEACAM5 in Western Blot, IP, IHC-P. Suitable for Human.

- BSA free for higher signal-to noise ratio
- Recombinant format for unrivalled batch-batch consistency

View Alternative Names

CD66e, CEA, CEACAM5, Cell adhesion molecule CEACAM5, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, Meconium antigen 100, CEA cell adhesion molecule 5

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)

Sample : FFPE section of bladder carcinoma. Antibody : Rabbit anti-CEACAM5 recombinant monoclonal antibody [BLR198J] (A700-198 lot 1). Secondary : HRP-conjugated goat anti-rabbit IgG (A120-501P).

Supplier Data

Immunocytochemistry - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)

Detection of human CEACAM5 by immunocytochemistry Sample : FFPE section of KM12 (colon carcinoma) cells. Antibody : Rabbit anti-CEACAM5 recombinant monoclonal antibody [BLR198J]. Secondary : HRPconjugated goat anti-rabbit IgG

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)

Sample : FFPE sections of liver carcinoma. Antibody : Rabbit anti-CEACAM5 recombinant monoclonal antibody [BLR198J] (A700-198 lot

(left -negative) and Rabbit anti-CEACAM1 recombinant monoclonal antibody [BLR090G] (A700-090-1) (right -positive). Secondary : HRP-conjugated goat anti-rabbit IgG (A120-501P).

Supplier Data

Immunocytochemistry - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)

Detection of human CEACAM5 by immunocytochemistry Sample : FFPE section of over-expressing CEACAM1, CEACAM3, CEACAM5 and CEACAM6 cells. Antibody : Rabbit anti-CEACAM5 recombinant monoclonal antibody [BLR198J]. Secondary : HRP-conjugated goat anti-rabbit IgG

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Immunoprecipitation - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)

Samples : Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from TT cells. Antibodies : Rabbit anti-CEACAM5 recombinant monoclonal antibody [BLR198J] used for IP at 20 µl/mg lysate. CEACAM5 was also immunoprecipitated by a second antibody against a different epitope of CEACAM5. For blotting immunoprecipitated CEACAM5, A700-198 was used at 1 : 1000. Chemiluminescence with an exposure time of 75 seconds.

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Supplier Data

Western blot - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)

Samples : Whole cell lysate (50 µg) from KM12 (10 µg), MCF-7, HEK293T, TT (2 µg), and HT-29 cells. Antibody : Rabbit anti-CEACAM5 recombinant monoclonal antibody [BLR198J] used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 75 seconds. Lower Panel : Rabbit anti-COPB2 antibody

All lanes:

Western blot - Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055) at 1/1000 dilution

Lane 1:

KM12 Whole cell lysate at 10 µg

Lane 2:

MCF-7 Whole cell lysate at 50 µg

Lane 3:

HEK-293T Whole cell lysate at 50 µg

Lane 4:

TT Whole cell lysate at 2 µg

Lane 5:

HT-29 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR198J

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, ICC, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CEACAM5 aa 1-700. The exact immunogen used to generate this antibody is proprietary information.

P06731

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-CEACAM5 antibody [BLR198J] - BSA free (ab314055) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of CEACAM5?
Anti-CEACAM5 [BLR198J] - BSA free (ab314055) specifically detects a band for CEACAM5 (UniProt: P06731) at a molecular weight of 76kDa.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEACAM5 also known as Carcinoembryonic Antigen (CEA) or CD66e is a glycoprotein found mainly on the surface of epithelial cells. This protein has a mass of approximately 180 kDa. It gets expressed in a variety of tissues with high expression in the gastrointestinal tract including the colon and rectum. CEACAM5 is a member of the immunoglobulin superfamily and plays a role in cell adhesion influencing how cells stick to each other.

Biological function summary

CEACAM5 contributes to cellular processes involving cell adhesion and immune response modulation. This protein operates as part of a larger group of cell adhesion molecules and does not function as part of a complex. CEACAM5 can affect the interactions between cells and the extracellular matrix which has implications for tissue structuring and integrity. It helps the immune system recognize and respond to infectious agents or cellular anomalies.

Pathways

CEACAM5 involvement extends to immune response regulation and cell adhesion pathways. It interacts functionally with proteins like E-cadherin in these pathways which contribute to maintaining epithelial integrity. These pathways are critical for normal tissue maintenance and immune system function especially in mucosal surfaces where pathogen entry occurs. Understanding CEACAM5 interactions provides insight into cellular communication and adhesion mechanisms.

CEACAM5 is associated with colorectal cancer and lung adenocarcinoma. Its overexpression and release into the bloodstream make it a popular biomarker for these diseases. CEACAM5 is used in clinical applications such as CEA ELISA assays for cancer detection and monitoring. In colorectal cancer CEACAM5 interacts with proteins like CEACAM6 to promote tumor invasiveness and metastasis. Anti-CEACAM5 therapeutics including antibody-drug conjugates (anti-CEACAM5 ADC) are being developed to target and treat associated malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed : 10864933, PubMed : 10910050, PubMed : 2803308). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed : 2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed : 10910050).. (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.

See full target information CEACAM5

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CEACAM5 antibody [BLR198J] - BSA free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)

Immunocytochemistry - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)

Immunocytochemistry - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)

Immunoprecipitation - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)

Western blot - Anti-CEACAM5 antibody [BLR198J] - BSA free (AB314055)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise