Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (AB317619)

This data was developed using ab317618, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling CEACAM5 with ab317618 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse testis. The section was incubated with ab317618 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (AB317619)

This data was developed using ab317618, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling CEACAM5 with ab317618 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse stomach. The section was incubated with ab317618 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (AB317619)

This data was developed using ab317618, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling CEACAM5 with ab317618 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse colon. The section was incubated with ab317618 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (AB317619)

This data was developed using ab317618, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : spleen, liver, testis, lung(PMID : 15194045)

The expression profile observed is consistent with differential glycosylation of this protein (PMID : 1717152).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane1 : 15 seconds, lanes 2-6 : 180 seconds

All lanes:

Western blot - Anti-CEACAM5 antibody [EPR28723-86] (<a href='/en-us/products/primary-antibodies/ceacam5-antibody-epr28723-86-ab317618'>ab317618</a>) at 1/1000 dilution

Lane 1:

Mouse colon cancer tissue lysate at 20 µg

Lane 2:

Mouse colon tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Mouse liver tissue lysate at 20 µg

Lane 5:

Mouse testis tissue lysate at 20 µg

Lane 6:

Mouse lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 106 kDa,130-180 kDa,36 kDa

false

• Dot

Supplier Data

Dot Blot - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (AB317619)

This data was developed using ab317618, the same antibody clone in a different buffer formulation.

Dot blot analysis of CEACAM5 using ab317618 at 1 : 1000 (0.499 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 10,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody doesn't cross activity with Ceacam1, Ceacam2, Ceacam10, Ceacam15 and Ceacam16.

All lanes:

Dot Blot - Anti-CEACAM5 antibody [EPR28723-86] (<a href='/en-us/products/primary-antibodies/ceacam5-antibody-epr28723-86-ab317618'>ab317618</a>) at 1/1000 dilution

Lane 1:

Mouse Ceacam5 protein (immunogen)

Lane 2:

Mouse Ceacam10 peptide a (aa 50-61)

Lane 3:

Mouse Ceacam10 peptide b (aa 91-107)

Lane 4:

Mouse Ceacam10 peptide c (aa 161-179)

Lane 5:

Mouse Ceacam10 peptide d (aa 212-230)

Lane 6:

Mouse Ceacam1 peptide a (aa 357-375)

Lane 7:

Mouse Ceacam1 peptide b (aa 381-401)

Lane 8:

Mouse Ceacam2 peptide a (aa 381-403)

Lane 9:

Mouse Ceacam15 peptide a (aa 186-200)

Lane 10:

Mouse Ceacam15 peptide b (aa 201-221)

Lane 11:

Mouse Ceacam16 peptide a (aa 79-90)

Lane 12:

Mouse Ceacam16 peptide b (aa 373-387)

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

false

Exposure time: 180s