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Rabbit Recombinant Monoclonal CEACAM5 antibody. Carrier free. Suitable for Dot, WB, IHC-P and reacts with Recombinant full length protein - Mouse, Mouse samples.

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Images

Western blot - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (AB317619), expandable thumbnail
  • Dot Blot - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (AB317619), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (AB317619), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (AB317619), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (AB317619), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotWBIHC-PFlow CytIPICC/IF
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Expected
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant full length protein - Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Recombinant full length protein - Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Recombinant full length protein - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Rat, Recombinant full length protein - Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Recombinant full length protein - Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Recombinant full length protein - Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Recombinant full length protein - Mouse, Human, Rat

Dilution info

-

Notes

-

Target data

Function

Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells.

Alternative names

Carcinoembryonic antigen-related cell adhesion molecule 5, Pregnancy-specific glycoprotein 30

Recommended products

Rabbit Recombinant Monoclonal CEACAM5 antibody. Carrier free. Suitable for Dot, WB, IHC-P and reacts with Recombinant full length protein - Mouse, Mouse samples.

Alternative names

Carcinoembryonic antigen-related cell adhesion molecule 5, Pregnancy-specific glycoprotein 30

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR28723-86

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab317619 is the carrier-free version of Anti-CEACAM5 antibody [EPR28723-86] ab317618.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CEACAM5 also known as Carcinoembryonic Antigen (CEA) or CD66e is a glycoprotein found mainly on the surface of epithelial cells. This protein has a mass of approximately 180 kDa. It gets expressed in a variety of tissues with high expression in the gastrointestinal tract including the colon and rectum. CEACAM5 is a member of the immunoglobulin superfamily and plays a role in cell adhesion influencing how cells stick to each other.

Biological function summary

CEACAM5 contributes to cellular processes involving cell adhesion and immune response modulation. This protein operates as part of a larger group of cell adhesion molecules and does not function as part of a complex. CEACAM5 can affect the interactions between cells and the extracellular matrix which has implications for tissue structuring and integrity. It helps the immune system recognize and respond to infectious agents or cellular anomalies.

Pathways

CEACAM5 involvement extends to immune response regulation and cell adhesion pathways. It interacts functionally with proteins like E-cadherin in these pathways which contribute to maintaining epithelial integrity. These pathways are critical for normal tissue maintenance and immune system function especially in mucosal surfaces where pathogen entry occurs. Understanding CEACAM5 interactions provides insight into cellular communication and adhesion mechanisms.

Associated diseases and disorders

CEACAM5 is associated with colorectal cancer and lung adenocarcinoma. Its overexpression and release into the bloodstream make it a popular biomarker for these diseases. CEACAM5 is used in clinical applications such as CEA ELISA assays for cancer detection and monitoring. In colorectal cancer CEACAM5 interacts with proteins like CEACAM6 to promote tumor invasiveness and metastasis. Anti-CEACAM5 therapeutics including antibody-drug conjugates (anti-CEACAM5 ADC) are being developed to target and treat associated malignancies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (ab317619), expandable thumbnail

    Western blot - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (ab317619)

    This data was developed using Anti-CEACAM5 antibody [EPR28723-86] ab317618, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: spleen, liver, testis, lung(PMID:15194045)

    The expression profile observed is consistent with differential glycosylation of this protein (PMID: 1717152).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lane1:15 seconds, lanes 2-6:180 seconds

    All lanes: Western blot - Anti-CEACAM5 antibody [EPR28723-86] (Anti-CEACAM5 antibody [EPR28723-86] ab317618) at 1/1000 dilution

    Lane 1: Mouse colon cancer tissue lysate at 20 µg

    Lane 2: Mouse colon tissue lysate at 20 µg

    Lane 3: Mouse spleen tissue lysate at 20 µg

    Lane 4: Mouse liver tissue lysate at 20 µg

    Lane 5: Mouse testis tissue lysate at 20 µg

    Lane 6: Mouse lung tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 106 kDa, 130-180 kDa, 36 kDa

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: spleen, liver, testis, lung(PMID:15194045)

    The expression profile observed is consistent with differential glycosylation of this protein (PMID: 1717152).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lane1:15 seconds, lanes 2-6:180 seconds

  • Dot Blot - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (ab317619), expandable thumbnail

    Dot Blot - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (ab317619)

    This data was developed using Anti-CEACAM5 antibody [EPR28723-86] ab317618, the same antibody clone in a different buffer formulation.

    Dot blot analysis of CEACAM5 using Anti-CEACAM5 antibody [EPR28723-86] ab317618 at 1:1000 (0.499 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:10,000 dilution.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody doesn't cross activity with Ceacam1, Ceacam2, Ceacam10, Ceacam15 and Ceacam16.

    All lanes: Dot Blot - Anti-CEACAM5 antibody [EPR28723-86] (Anti-CEACAM5 antibody [EPR28723-86] ab317618) at 1/1000 dilution

    Lane 1: Mouse Ceacam5 protein (immunogen)

    Lane 2: Mouse Ceacam10 peptide a (aa 50-61)

    Lane 3: Mouse Ceacam10 peptide b (aa 91-107)

    Lane 4: Mouse Ceacam10 peptide c (aa 161-179)

    Lane 5: Mouse Ceacam10 peptide d (aa 212-230)

    Lane 6: Mouse Ceacam1 peptide a (aa 357-375)

    Lane 7: Mouse Ceacam1 peptide b (aa 381-401)

    Lane 8: Mouse Ceacam2 peptide a (aa 381-403)

    Lane 9: Mouse Ceacam15 peptide a (aa 186-200)

    Lane 10: Mouse Ceacam15 peptide b (aa 201-221)

    Lane 11: Mouse Ceacam16 peptide a (aa 79-90)

    Lane 12: Mouse Ceacam16 peptide b (aa 373-387)

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Exposure time: 180s

    Dot blot analysis of CEACAM5 using Anti-CEACAM5 antibody [EPR28723-86] ab317618 at 1:1000 (0.499 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:10,000 dilution.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody doesn't cross activity with Ceacam1, Ceacam2, Ceacam10, Ceacam15 and Ceacam16.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (ab317619), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (ab317619)

    This data was developed using Anti-CEACAM5 antibody [EPR28723-86] ab317618, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling CEACAM5 with Anti-CEACAM5 antibody [EPR28723-86] ab317618 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse colon. The section was incubated with Anti-CEACAM5 antibody [EPR28723-86] ab317618 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (ab317619), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (ab317619)

    This data was developed using Anti-CEACAM5 antibody [EPR28723-86] ab317618, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling CEACAM5 with Anti-CEACAM5 antibody [EPR28723-86] ab317618 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on mouse stomach. The section was incubated with Anti-CEACAM5 antibody [EPR28723-86] ab317618 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (ab317619), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [EPR28723-86] - BSA and Azide free (ab317619)

    This data was developed using Anti-CEACAM5 antibody [EPR28723-86] ab317618, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling CEACAM5 with Anti-CEACAM5 antibody [EPR28723-86] ab317618 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on mouse testis. The section was incubated with Anti-CEACAM5 antibody [EPR28723-86] ab317618 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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