AB229074

Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal CEACAM5 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF, IHC-P, WB and reacts with Human, Transfected cell line - Human samples. Cited in 1 publication.

View Alternative Names

CD66e, CEA, CEACAM5, Cell adhesion molecule CEACAM5, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, Meconium antigen 100, CEA cell adhesion molecule 5, CD66c, NCA, CEACAM6, Cell adhesion molecule CEACAM6, Carcinoembryonic antigen-related cell adhesion molecule 6, Non-specific crossreacting antigen, Normal cross-reacting antigen, CEA cell adhesion molecule 6, CD66a, BGP, BGP1, CEACAM1, Cell adhesion molecule CEACAM1, Biliary glycoprotein 1, Carcinoembryonic antigen-related cell adhesion molecule 1, BGP-1, CEA cell adhesion molecule 1, CD66b, CGM6, CEACAM8, Cell adhesion molecule CEACAM8, CD67 antigen, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, Non-specific cross-reacting antigen NCA-95, CEA cell adhesion molecule 8

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free (AB229074)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free (AB229074)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Carcino Embryonic Antigen CEA with ab207718 at 1/40,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining of spleen-infiltrating neutrophils (PMID : 22064717 and 19077207) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207718).

Immunocytochemistry/ Immunofluorescence - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free (AB229074)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free (AB229074)

Immunofluorescent analysis of 100% methanol fixed MCF7 (human breast adenocarcinoma epithelial cell) cells labeling Carcino Embryonic Antigen CEA with ab207718 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing membranous staining on MCF7 cell line.

Negative control for cells : PANC-1 .

Counterstained with ab195889 anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red) and DAPI (blue).

The negative control is the secondary antibody only.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207718).

Flow Cytometry - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free (AB229074)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free (AB229074)

Flow cytometric analysis of PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) (Left) / MCF7 (human breast adenocarcinoma epithelial cell) (Right) labeling Carcino Embryonic Antigen CEA with ab207718 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Cells were not fixed or permeabilized. Gated on total viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207718).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free (AB229074)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Carcino Embryonic Antigen CEA with ab207718 at 1/40,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human colon (PMID : 25042385) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207718).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free (AB229074)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Carcino Embryonic Antigen CEA with ab207718 at 1/40,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human colon cancer (PMID : 25042385) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207718).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free (AB229074)

Immunohistochemistry analysis of paraffin-embedded (A) HEK-293T cells transfected with a CEACAM1 expression vector containing a his tag, (B) HEK-293T cells transfected with a CEACAM3 expression vector containing a his tag, (C) HEK-293T cells transfected with a CEACAM5 expression vector containing a his tag, (D) HEK-293T cells transfected with a CEACAM6 expression vector containing a his tag, (E) HEK-293T cells transfected with a CEACAM8 expression vector containing a his tag, (F) HEK-293T cells transfected with empty vector containing a his tag, sections labelling CEACAM5 + CD66b + CEACAM1 + CEACAM6 with ab207718 at 1/5000 dilution. The section was incubated with ab207718 for 30 mins at room temperature. LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Positive stainng on (B) HEK-293T cells transfected with a CEACAM3 expression vector containing a his tag, (C) HEK-293T cells transfected with a CEACAM5 expression vector containing a his tag, (E) HEK-293T cells transfected with a CEACAM8 expression vector containing a his tag. Nearly no staining on (A) HEK-293T cells transfected with a CEACAM1 expression vector containing a his tag, (D) HEK-293T cells transfected with a CEACAM6 expression vector containing a his tag and (F) HEK-293T cells transfected with empty vector containing a his tag. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207718).

Unconjugated

Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20721

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab229074 cross-reacts with CEACAM 1, 6 and 8. There are other CEACAM family members but we only examined cross-reactivity with those having >55% homology with CEACAM5 following a BLAST search with the immunogen.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab229074 is the carrier-free version of ab207718.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEACAM5 also known as CEA or CD66e is part of the carcinoembryonic antigen family. It plays a role as a cell adhesion molecule facilitating intercellular binding. CEACAM5 is a glycoprotein with a molecular mass of approximately 180 kDa. You find CEACAM5 expression in normal epithelial tissues such as the colon stomach and tongue but it shows increased levels in various carcinoma cells. It is also identified as CEACAM6 and both of these linked proteins function as cell surface glycoproteins involved in cellular interactions.

Biological function summary

CEACAM5 proteins influence cell signaling and intercellular communication. They participate in immune response modulation and function as part of larger protein complexes. CEACAM5 acts as an anchor point for various cellular processes by associating with CEACAM1 and CEACAM6. By regulating cell adhesion CEACAM5 helps in maintaining normal cellular architecture and function within tissue environments.

Pathways

CEACAM5 is integral to pathways involving cell adhesion and signaling. It participates in immune system responses and the regulation of cell proliferation pathways including the MEK/ERK signaling cascade. CEACAM5 proteins interact with others like CEACAM1 sharing roles in cell signaling and communication networks that control adhesion dynamics and growth factor responses.

Excessive CEACAM5 expression links to colorectal and gastric cancers making it a useful cancer biomarker. CEACAM5-positive tumors often correlate with disease progression which highlights their potential as therapeutic targets. These proteins also interact with CD66b markers which are associated with neutrophil activation in inflammatory responses. Such associations with CEACAM1 and CEACAM6 in carcinogenic pathways further underline CEACAM5's relevance in cancer diagnostics and treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed : 10864933, PubMed : 10910050, PubMed : 2803308). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed : 2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed : 10910050).. (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.

See full target information CEACAM5

Additional targets

CEACAM8,CEACAM1,CEACAM6

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neuroscience bulletin 40:421-438 PubMed37864744

2023

Inflammatory Factor IL1α Induces Aberrant Astrocyte Proliferation in Spinal Cord Injury Through the Grin2c/Ca/CaMK2b Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Xia,Lu Ding,Changlin Zhang,Qi Xu,Ming Shi,Tianshun Gao,Feng-Quan Zhou,David Y B Deng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free (AB229074)

Immunocytochemistry/ Immunofluorescence - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free (AB229074)

Flow Cytometry - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free (AB229074)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free (AB229074)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free (AB229074)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 + CD66b + CEACAM1 + CEACAM6 antibody [EPR20721] - BSA and Azide free (AB229074)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Properties and storage information

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Shipped at conditions

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Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise