AB254157

Anti-CEACAM6 antibody [1H7-4B]

Recombinant
What is this?

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(1 Publication)

Mouse Recombinant Monoclonal CEAM6 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD66c, NCA, CEACAM6, Cell adhesion molecule CEACAM6, Carcinoembryonic antigen-related cell adhesion molecule 6, Non-specific crossreacting antigen, Normal cross-reacting antigen, CEA cell adhesion molecule 6

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] (AB254157)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] (AB254157)

Formalin-fixed, paraffin-embedded human colon tissue stained for CEACAM6 using ab254157 at 0.207μg/ml in immunohistochemical analysis. Positive staining on the human colon is observed. A ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) was used as the secondary antibody.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] (AB254157)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for CEACAM6 using ab254157 at 0.207μg/ml in immunohistochemical analysis. Positive staining on the human colon carcinoma is observed. A ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) was used as the secondary antibody.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Supplier Data

Western blot - Anti-CEACAM6 antibody [1H7-4B] (AB254157)

CEACAM6 protein (95 kd) was observed. The molecular weight is consistent with that has been described in the literature (PMID : 24040308).

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 15 secs; Lane 2 : 5 secs.

All lanes:

Western blot - Anti-CEACAM6 antibody [1H7-4B] (ab254157) at 0.413 µg/mL

Lane 1:

HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 37 kDa

Observed band size: 95 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1H7-4B

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.413 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.207 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEACAM6 also known as 9a6 CEACAM-6 and 1h7 is a cell surface glycoprotein weighing approximately 35-40 kDa. The protein plays a mechanical role as an intercellular adhesion molecule. It displays broad expression across multiple tissues with high levels found in epithelial cells of the gastrointestinal tract respiratory tract and in leukocytes.

Biological function summary

CEACAM6 acts in cell signaling and adhesion processes. It often forms complexes with other members of the CEACAM family such as CEACAM5. This protein is involved in the regulation of cell-cell adhesion which impacts cell migration and organization. It also influences immune reactions by modulating the interactions between pathogens and host cells.

Pathways

CEACAM6 integrates into pathways associated with cell adhesion and immune regulation. It engages in the PI3K-Akt signaling pathway tightly interacting with proteins such as CEACAM1 and CEACAM5. Through these interactions CEACAM6 plays an essential role in cell survival proliferation and immune responses.

CEACAM6 shows significant links with cancer especially colorectal and pancreatic cancers. Overexpression of this protein correlates with tumor progression and metastasis. Additionally in inflammatory bowel disease CEACAM6 association with CEACAM7 often results in altered epithelial barrier function contributing to disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface glycoprotein that plays a role in cell adhesion and tumor progression (PubMed : 10910050, PubMed : 11590190, PubMed : 1378450, PubMed : 16204051, PubMed : 2022629, PubMed : 2803308, PubMed : 8776764). Intercellular adhesion occurs in a calcium- and fibronectin-independent manner (PubMed : 16204051, PubMed : 2022629). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8 (PubMed : 11590190, PubMed : 16204051, PubMed : 2022629, PubMed : 2803308, PubMed : 8776764). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed : 8776764). Plays a role in neutrophil adhesion to cytokine-activated endothelial cells (PubMed : 1378450). Plays a role in cell migration and cell adhesion to endothelial cells (PubMed : 16204051).

See full target information CEACAM6

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed39824529

2025

Pancreatic cancer cell-intrinsic transglutaminase-2 promotes T cell suppression through microtubule-dependent secretion of immunosuppressive cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Anton Lahusen,Nora Minhöfer,Kim-André Lohse,Christine Blechner,Jessica Lindenmayer,Tim Eiseler,Anton Wellstein,Alexander Kleger,Thomas Seufferlein,Sabine Windhorst,Yuan-Na Lin

View all publications

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Assay Kits

AB283545

Human CEACAM6 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CEACAM6 ELISA Kit (AB283545)

Primary Antibodies

AB133633

Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [EPCEAR7] (AB133633)

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB205270

Anti-active YAP1 antibody [EPR19812]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-active YAP1 antibody [EPR19812] (AB205270)

Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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