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Mouse Recombinant Monoclonal CEAM6 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (AB255910), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (AB255910), expandable thumbnail
  • Western blot - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (AB255910), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IFIHC-P
Human
Not recommended
Tested
Not recommended
Tested

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

0.413 µg/mL

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.207 µg/mL

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Cell surface glycoprotein that plays a role in cell adhesion and tumor progression (PubMed:10910050, PubMed:11590190, PubMed:1378450, PubMed:16204051, PubMed:2022629, PubMed:2803308, PubMed:8776764). Intercellular adhesion occurs in a calcium- and fibronectin-independent manner (PubMed:16204051, PubMed:2022629). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8 (PubMed:11590190, PubMed:16204051, PubMed:2022629, PubMed:2803308, PubMed:8776764). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed:8776764). Plays a role in neutrophil adhesion to cytokine-activated endothelial cells (PubMed:1378450). Plays a role in cell migration and cell adhesion to endothelial cells (PubMed:16204051).

Alternative names

Recommended products

Mouse Recombinant Monoclonal CEAM6 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

1H7-4B

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab255910 is the carrier-free version of Anti-CEACAM6 antibody [1H7-4B] ab254157.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CEACAM6 also known as 9a6 CEACAM-6 and 1h7 is a cell surface glycoprotein weighing approximately 35-40 kDa. The protein plays a mechanical role as an intercellular adhesion molecule. It displays broad expression across multiple tissues with high levels found in epithelial cells of the gastrointestinal tract respiratory tract and in leukocytes.

Biological function summary

CEACAM6 acts in cell signaling and adhesion processes. It often forms complexes with other members of the CEACAM family such as CEACAM5. This protein is involved in the regulation of cell-cell adhesion which impacts cell migration and organization. It also influences immune reactions by modulating the interactions between pathogens and host cells.

Pathways

CEACAM6 integrates into pathways associated with cell adhesion and immune regulation. It engages in the PI3K-Akt signaling pathway tightly interacting with proteins such as CEACAM1 and CEACAM5. Through these interactions CEACAM6 plays an essential role in cell survival proliferation and immune responses.

Associated diseases and disorders

CEACAM6 shows significant links with cancer especially colorectal and pancreatic cancers. Overexpression of this protein correlates with tumor progression and metastasis. Additionally in inflammatory bowel disease CEACAM6 association with CEACAM7 often results in altered epithelial barrier function contributing to disease pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (ab255910), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (ab255910)

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling CEACAM6 with Anti-CEACAM6 antibody [1H7-4B] ab254157 at 0.207μg/ml, followed by a Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879) ready to use. Positive staining on the human colon carcinoma is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879) ready to use.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CEACAM6 antibody [1H7-4B] ab254157).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (ab255910), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (ab255910)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CEACAM6 with Anti-CEACAM6 antibody [1H7-4B] ab254157 at 0.207μg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879) ready to use. Positive staining on the human colon is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879) ready to use.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CEACAM6 antibody [1H7-4B] ab254157).

  • Western blot - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (ab255910), expandable thumbnail

    Western blot - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (ab255910)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane1: 15 seconds; Lane2: 5.5 seconds.

    CEACAM6 protein (95 kd) was observed. The molecular weight is consistent with that has been described in the literature (PMID: 24040308).

    All lanes: Western blot - Anti-CEACAM6 antibody [1H7-4B] (Anti-CEACAM6 antibody [1H7-4B] ab254157) at 0.413 µg/mL

    Lane 1: HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 37 kDa

    Observed band size: 95 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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