Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (AB255910)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CEACAM6 with ab254157 at 0.207μg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on the human colon is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254157).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (AB255910)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling CEACAM6 with ab254157 at 0.207μg/ml, followed by a Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on the human colon carcinoma is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254157).

• WB

Supplier Data

Western blot - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (AB255910)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lane1 : 15 seconds; Lane2 : 5.5 seconds.

CEACAM6 protein (95 kd) was observed. The molecular weight is consistent with that has been described in the literature (PMID : 24040308).

All lanes:

Western blot - Anti-CEACAM6 antibody [1H7-4B] (<a href='/en-us/products/primary-antibodies/ceacam6-antibody-1h7-4b-ab254157'>ab254157</a>) at 0.413 µg/mL

Lane 1:

HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 37 kDa

Observed band size: 95 kDa

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