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Mouse Monoclonal CEAM6 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Cell preparation containing CEACAM6 protein.


Images

Immunocytochemistry/ Immunofluorescence - Anti-CEACAM6 antibody [9A6] (AB78029), expandable thumbnail
  • Western blot - Anti-CEACAM6 antibody [9A6] (AB78029), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 99.8% PBS, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing CEACAM6 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P40199

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/200.00000
Notes

-

Associated Products

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Target data

Function

Cell surface glycoprotein that plays a role in cell adhesion and tumor progression (PubMed:10910050, PubMed:11590190, PubMed:1378450, PubMed:16204051, PubMed:2022629, PubMed:2803308, PubMed:8776764). Intercellular adhesion occurs in a calcium- and fibronectin-independent manner (PubMed:16204051, PubMed:2022629). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8 (PubMed:11590190, PubMed:16204051, PubMed:2022629, PubMed:2803308, PubMed:8776764). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed:8776764). Plays a role in neutrophil adhesion to cytokine-activated endothelial cells (PubMed:1378450). Plays a role in cell migration and cell adhesion to endothelial cells (PubMed:16204051).

Alternative names

CD66c, NCA, CEACAM6, Cell adhesion molecule CEACAM6, Carcinoembryonic antigen-related cell adhesion molecule 6, Non-specific crossreacting antigen, Normal cross-reacting antigen, CEA cell adhesion molecule 6

Recommended products

Mouse Monoclonal CEAM6 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Cell preparation containing CEACAM6 protein.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing CEACAM6 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P40199
Clone number
9A6
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

ab78029 is purified.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CEACAM6 also known as 9a6 CEACAM-6 and 1h7 is a cell surface glycoprotein weighing approximately 35-40 kDa. The protein plays a mechanical role as an intercellular adhesion molecule. It displays broad expression across multiple tissues with high levels found in epithelial cells of the gastrointestinal tract respiratory tract and in leukocytes.

Biological function summary

CEACAM6 acts in cell signaling and adhesion processes. It often forms complexes with other members of the CEACAM family such as CEACAM5. This protein is involved in the regulation of cell-cell adhesion which impacts cell migration and organization. It also influences immune reactions by modulating the interactions between pathogens and host cells.

Pathways

CEACAM6 integrates into pathways associated with cell adhesion and immune regulation. It engages in the PI3K-Akt signaling pathway tightly interacting with proteins such as CEACAM1 and CEACAM5. Through these interactions CEACAM6 plays an essential role in cell survival proliferation and immune responses.

Associated diseases and disorders

CEACAM6 shows significant links with cancer especially colorectal and pancreatic cancers. Overexpression of this protein correlates with tumor progression and metastasis. Additionally in inflammatory bowel disease CEACAM6 association with CEACAM7 often results in altered epithelial barrier function contributing to disease pathogenesis.

Product promise

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2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-CEACAM6 antibody [9A6] (ab78029), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CEACAM6 antibody [9A6] (ab78029)

    Immunofluorescence of THP-1 cells with (A) mouse IgG1, κ isotype control (Negative) or (B-D) CEACAM6 primary antibody (ab78029). Alexa Fluor® 488 (Green) Goat anti-Mouse IgG was used as secondary antibody. Nuclei were counterstained with DAPI. The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/12, and (B-D) is 1/15. Concentrations for (A, B) is 1/100, (C) is 1/200 and (D) is 1:500.

  • Western blot - Anti-CEACAM6 antibody [9A6] (ab78029), expandable thumbnail

    Western blot - Anti-CEACAM6 antibody [9A6] (ab78029)

    HRP anti-β-actin was used as a loading control 1:8000

    All lanes: Western blot - Anti-CEACAM6 antibody [9A6] (ab78029) at 1/500 dilution

    Lane 1: HeLa at 15 µg

    Lane 2: THP-1 at 15 µg

    Lane 3: HL-60 (Low expression negative control) at 15 µg

    Secondary

    All lanes: HRP Goat anti-Mouse

    Predicted band size: 37 kDa

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Product protocols

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