AB78029

Anti-CEACAM6 antibody [9A6]

Name: Anti-CEACAM6 antibody [9A6] (ab78029) | Abcam
Brand: Abcam Limited
SKU: AB78029
Price: 435 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(13 Publications)

Anti-CEACAM6 antibody [9A6] (ab78029) is a mouse monoclonal antibody detecting CEACAM6 in Western Blot, ICC/IF. Suitable for Human.

- Trusted since 2009

View Alternative Names

CD66c, NCA, CEACAM6, Cell adhesion molecule CEACAM6, Carcinoembryonic antigen-related cell adhesion molecule 6, Non-specific crossreacting antigen, Normal cross-reacting antigen, CEA cell adhesion molecule 6

2 Images

Supplier Data

Western blot - Anti-CEACAM6 antibody [9A6] (AB78029)

HRP anti-β-actin was used as a loading control 1 : 8000

All lanes:

Western blot - Anti-CEACAM6 antibody [9A6] (ab78029) at 1/500 dilution

Lane 1:

HeLa at 15 µg

Lane 2:

THP-1 at 15 µg

Lane 3:

HL-60 (Low expression negative control) at 15 µg

Secondary

All lanes:

HRP Goat anti-Mouse

Predicted band size: 37 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-CEACAM6 antibody [9A6] (AB78029)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CEACAM6 antibody [9A6] (AB78029)

Immunofluorescence of THP-1 cells with (A) mouse IgG1, κ isotype control (Negative) or (B-D) CEACAM6 primary antibody (ab78029). Alexa Fluor® 488 (Green) Goat anti-Mouse IgG was used as secondary antibody. Nuclei were counterstained with DAPI. The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/12, and (B-D) is 1/15. Concentrations for (A, B) is 1/100, (C) is 1/200 and (D) is 1 : 500.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9A6

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Cell preparation containing CEACAM6 protein. The exact immunogen used to generate this antibody is proprietary information.

P40199

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-CEACAM6 antibody [9A6] (ab78029) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of CEACAM6?
Anti-CEACAM6 [9A6] (ab78029) specifically detects a band for CEACAM6 (UniProt: P40199) at a molecular weight of 37kDa.

Trusted by the scientific community
Anti-CEACAM6 [9A6] (ab78029) was first used in a scientific publication in 2009 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

ab78029 is purified.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: 99.8% PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEACAM6 also known as 9a6 CEACAM-6 and 1h7 is a cell surface glycoprotein weighing approximately 35-40 kDa. The protein plays a mechanical role as an intercellular adhesion molecule. It displays broad expression across multiple tissues with high levels found in epithelial cells of the gastrointestinal tract respiratory tract and in leukocytes.

Biological function summary

CEACAM6 acts in cell signaling and adhesion processes. It often forms complexes with other members of the CEACAM family such as CEACAM5. This protein is involved in the regulation of cell-cell adhesion which impacts cell migration and organization. It also influences immune reactions by modulating the interactions between pathogens and host cells.

Pathways

CEACAM6 integrates into pathways associated with cell adhesion and immune regulation. It engages in the PI3K-Akt signaling pathway tightly interacting with proteins such as CEACAM1 and CEACAM5. Through these interactions CEACAM6 plays an essential role in cell survival proliferation and immune responses.

CEACAM6 shows significant links with cancer especially colorectal and pancreatic cancers. Overexpression of this protein correlates with tumor progression and metastasis. Additionally in inflammatory bowel disease CEACAM6 association with CEACAM7 often results in altered epithelial barrier function contributing to disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface glycoprotein that plays a role in cell adhesion and tumor progression (PubMed : 10910050, PubMed : 11590190, PubMed : 1378450, PubMed : 16204051, PubMed : 2022629, PubMed : 2803308, PubMed : 8776764). Intercellular adhesion occurs in a calcium- and fibronectin-independent manner (PubMed : 16204051, PubMed : 2022629). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8 (PubMed : 11590190, PubMed : 16204051, PubMed : 2022629, PubMed : 2803308, PubMed : 8776764). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed : 8776764). Plays a role in neutrophil adhesion to cytokine-activated endothelial cells (PubMed : 1378450). Plays a role in cell migration and cell adhesion to endothelial cells (PubMed : 16204051).

See full target information CEACAM6

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Gut microbes 17:2526136 PubMed40624731

2025

The rs1893217 IBD risk allele increases susceptibility to AIEC invasion by a JAK-STAT-CEACAM6 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Pritha Chatterjee,Vinicius Canale,Stephanie J King,Ali Shawki,Hillmin Lei,Alina N Santos,Michael Haddad,Casey Gries,Dermot P B McGovern,James Borneman,Declan F McCole

NPJ precision oncology 9:115 PubMed40263546

2025

Cytokine and epigenetic regulation of CEACAM6 mediates EGFR-driven signaling and drug response in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Yi Zheng,Yen-Ting Lin,Yen-Shou Kuo,Yen-Ju Lin,Ming-Han Kuo,Tsai-Wang Huang,Yi-Shing Shieh,Yenlin Huang,Yu-Ting Chou

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 28:422-441 PubMed39918687

2025

Identification of CD66c as a potential target in gastroesophageal junction cancer for antibody-drug conjugate development.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Zhang,Changjuan Tao,Hanfei Xie,Liu Yang,Ye Lu,Yun Xi,Shili Yao,Li Yuan,Peng Guo,Xiangdong Cheng

Cell cycle (Georgetown, Tex.) 21:1091-1102 PubMed35220873

2022

Circ_0008035 promotes the progression of gastric cancer via the regulation of miR-1256/CEACAM6 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoyang Chu,Xianli Liu,Zhiguo Zhao,Zhijie Shi

Therapeutic advances in medical oncology 14:17588359211072621 PubMed35082925

2022

Prognostic value of the immune target CEACAM6 in cancer: a meta-analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel Burgos,Iván Cavero-Redondo,Celia Álvarez-Bueno,Eva María Galán-Moya,Atanasio Pandiella,Eitan Amir,Alberto Ocaña

Oncology letters 20:2677-2688 PubMed32782584

2020

Effect of silencing on the biological behavior of human gallbladder cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chengming Tian,Bingye Zhang,Chunlin Ge

American journal of physiology. Cell physiology 318:C83-C93 PubMed31644306

2019

Nucleolin mediates the binding of cancer cells to L-selectin under conditions of lymphodynamic shear stress.

Applications

Unspecified application

Species

Unspecified reactive species

Tovë M Goldson,Kevin L Turner,Yinan Huang,Grady E Carlson,Emily G Caggiano,Andres F Oberhauser,Susan M Fennewald,Monica M Burdick,Vicente A Resto

Frontiers in oncology 9:216 PubMed31001480

2019

Heparanase Promotes Tumor Growth and Liver Metastasis of Colorectal Cancer Cells by Activating the p38/MMP1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Liu,Zhi-Hang Zhou,Wen Li,Shi-Kun Zhang,Jing Li,Ming-Ju Zhou,Jin-Wen Song

Scientific reports 7:10773 PubMed28883649

2017

Dual role of carcinoembryonic antigen-related cell adhesion molecule 6 expression in predicting the overall survival of gastric cancer patients.

Applications

IHC

Species

Human

Mingde Zang,Lei Hu,Shu Cao,Zhiyuan Fan,Li Pang,Jianfang Li,Liping Su,Chen Li,Wentao Liu,Qinlong Gu,Zhenggang Zhu,Min Yan,Bingya Liu

Journal of pathology and translational medicine 49:112-7 PubMed25812733

2015

The Diagnostic Usefulness of HMGA2, Survivin, CEACAM6, and SFN/14-3-3 δ in Follicular Thyroid Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Min Hye Jang,Kyeong Cheon Jung,Hye Sook Min

View all publications

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