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AB275022

Anti-CEACAM6 antibody [EPR23956-80]

  • 20ul selling size
  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • What is this?

5

(1 Review)

|

(4 Publications)

Rabbit Recombinant Monoclonal CEAM6 antibody. Suitable for ICC/IF, IP, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CD66c, NCA, CEACAM6, Cell adhesion molecule CEACAM6, Carcinoembryonic antigen-related cell adhesion molecule 6, Non-specific crossreacting antigen, Normal cross-reacting antigen, CEA cell adhesion molecule 6

9 Images
Immunocytochemistry/ Immunofluorescence - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized BxPC-3 BxPC-3 (human pancreas adenocarcinoma cell) cells labelling CEACAM6 with ab275022 at 1/500 (1.048 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplamic staining in BxPC-3 cell line. Low expression control : LNCaP : PMID : 16204051. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CEACAM6 with ab275022 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human spleen. The section was incubated with ab275022 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling CEACAM6 with ab275022 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon cancer (PMID : 27284373). The section was incubated with ab275022 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Flow Cytometry - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)

Flow cytometric analysis of LNCaP (human prostate carcinoma epithelial cell, Left) / BxPC-3 (human pancreas adenocarcinoma epithelial cell, Right) cells labelling CEACAM6 with ab275022 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control : LNCaP.

Gated on viable cells.

Immunoprecipitation - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)
  • IP

Unknown

Immunoprecipitation - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)

CEACAM6 was immunoprecipitated from 0.35 mg BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate with ab275022 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275022 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate 10 ug

Lane 2 : ab275022 IP in BxPC-3 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab275022 in BxPC-3 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-CEACAM6 antibody [EPR23956-80] (ab275022)

Predicted band size: 37 kDa

Observed band size: 40-100 kDa

false

Immunoprecipitation - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)
  • IP

Unknown

Immunoprecipitation - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)

CEACAM6 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with ab275022 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275022 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : A549 (human lung carcinoma epithelial cell) whole cell lysate 10 ug

Lane 2 : ab275022 IP in A549 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab275022 in A549 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-CEACAM6 antibody [EPR23956-80] (ab275022)

Predicted band size: 37 kDa

Observed band size: 40-100 kDa

false

Western blot - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)
  • WB

Unknown

Western blot - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-2 : 15 seconds; Lane3 : 3 seconds; Lane 4 : 26 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 19244123, 28892050, 16204051).

Low expression control : LNCaP (PMID : 16204051).

All lanes:

Western blot - Anti-CEACAM6 antibody [EPR23956-80] (ab275022) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 37 kDa

Observed band size: 40-100 kDa

false

Western blot - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)
  • WB

Unknown

Western blot - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 26 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 19244123, 28892050, 16204051).

All lanes:

Western blot - Anti-CEACAM6 antibody [EPR23956-80] (ab275022) at 1/1000 dilution

All lanes:

Human lung tissue lysate at 20 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 37 kDa

Observed band size: 40-100 kDa

false

Western blot - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)
  • WB

Unknown

Western blot - Anti-CEACAM6 antibody [EPR23956-80] (AB275022)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 15 seconds.

The other family members have lower homology than the tested proteins.

All lanes:

Western blot - Anti-CEACAM6 antibody [EPR23956-80] (ab275022) at 1/1000 dilution

Lane 1:

His-tagged human CEACAM6 recombinant protein, 20ng

Lane 2:

His-tagged human CEACAM1 recombinant protein (aa35-428), 20ng

Lane 3:

His-tagged human CEACAM5 recombinant protein (aa35-685), 20ng

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 37 kDa

false

  • Carrier free

    Anti-CEACAM6 antibody [EPR23956-80] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CEACAM6 antibody [EPR23956-80]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CEACAM6 antibody [EPR23956-80]

  • 578 PE

    PE Anti-CEACAM6 antibody [EPR23956-80]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23956-80

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB, Flow Cyt, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB283545

Human CEACAM6 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CEACAM6 ELISA Kit (AB283545)

  • 0
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Primary Antibodies

AB133633

Anti-CEACAM5 antibody [EPCEAR7]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM5 antibody [EPCEAR7] (AB133633)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB205270

Anti-active YAP1 antibody [EPR19812]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-active YAP1 antibody [EPR19812] (AB205270)

  • 5
  • 25
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEACAM6 also known as 9a6 CEACAM-6 and 1h7 is a cell surface glycoprotein weighing approximately 35-40 kDa. The protein plays a mechanical role as an intercellular adhesion molecule. It displays broad expression across multiple tissues with high levels found in epithelial cells of the gastrointestinal tract respiratory tract and in leukocytes.
Biological function summary

CEACAM6 acts in cell signaling and adhesion processes. It often forms complexes with other members of the CEACAM family such as CEACAM5. This protein is involved in the regulation of cell-cell adhesion which impacts cell migration and organization. It also influences immune reactions by modulating the interactions between pathogens and host cells.

Pathways

CEACAM6 integrates into pathways associated with cell adhesion and immune regulation. It engages in the PI3K-Akt signaling pathway tightly interacting with proteins such as CEACAM1 and CEACAM5. Through these interactions CEACAM6 plays an essential role in cell survival proliferation and immune responses.

CEACAM6 shows significant links with cancer especially colorectal and pancreatic cancers. Overexpression of this protein correlates with tumor progression and metastasis. Additionally in inflammatory bowel disease CEACAM6 association with CEACAM7 often results in altered epithelial barrier function contributing to disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface glycoprotein that plays a role in cell adhesion and tumor progression (PubMed : 10910050, PubMed : 11590190, PubMed : 1378450, PubMed : 16204051, PubMed : 2022629, PubMed : 2803308, PubMed : 8776764). Intercellular adhesion occurs in a calcium- and fibronectin-independent manner (PubMed : 16204051, PubMed : 2022629). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8 (PubMed : 11590190, PubMed : 16204051, PubMed : 2022629, PubMed : 2803308, PubMed : 8776764). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed : 8776764). Plays a role in neutrophil adhesion to cytokine-activated endothelial cells (PubMed : 1378450). Plays a role in cell migration and cell adhesion to endothelial cells (PubMed : 16204051).
See full target information CEACAM6
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 9:115 PubMed40263546

2025

Cytokine and epigenetic regulation of CEACAM6 mediates EGFR-driven signaling and drug response in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Yi Zheng,Yen-Ting Lin,Yen-Shou Kuo,Yen-Ju Lin,Ming-Han Kuo,Tsai-Wang Huang,Yi-Shing Shieh,Yenlin Huang,Yu-Ting Chou

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 28:422-441 PubMed39918687

2025

Identification of CD66c as a potential target in gastroesophageal junction cancer for antibody-drug conjugate development.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Zhang,Changjuan Tao,Hanfei Xie,Liu Yang,Ye Lu,Yun Xi,Shili Yao,Li Yuan,Peng Guo,Xiangdong Cheng

Cell reports. Medicine 4:101277 PubMed37944531

2023

Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxue Ren,Mingle Huang,Weixiang Weng,Yubin Xie,Yifan Wu,Shenghua Zhu,Ying Zhang,Dongming Li,Jiaming Lai,Shunli Shen,Jie Lin,Ming Kuang,Xiaoxing Li,Jun Yu,Lixia Xu

Frontiers in oncology 11:643669 PubMed34221964

2021

Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 Promotes Carcinogenesis of Gastric Cancer and Anti-CEACAM6 Fluorescent Probe Can Diagnose the Precancerous Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Fangmei An,Chuwei Zheng,Guoqiang Zhang,Liangyun Zhou,Yuqing Wu,Zheng Hou,Zhiyi Zhou,Ke Chen,Qiang Zhan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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