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AB134074

Anti-CEACAM6 antibody [EPR4403]

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(6 Publications)

Rabbit Recombinant Monoclonal CEAM6 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

CD66c, NCA, CEACAM6, Cell adhesion molecule CEACAM6, Carcinoembryonic antigen-related cell adhesion molecule 6, Non-specific crossreacting antigen, Normal cross-reacting antigen, CEA cell adhesion molecule 6

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [EPR4403] (AB134074)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [EPR4403] (AB134074)

Immunohistochemical analysis of paraffin embedded human colon tissue labelling CEACAM6 with ab134074 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-CEACAM6 antibody [EPR4403] (AB134074)
  • WB

Lab

Western blot - Anti-CEACAM6 antibody [EPR4403] (AB134074)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human CEACAM1, CEACAM5 or CEACAM8.

All lanes:

Western blot - Anti-CEACAM6 antibody [EPR4403] (ab134074) at 1/1000 dilution

Lane 1:

His-tagged human CEACAM1 recombinant protein at 0.02 µg

Lane 2:

His-tagged human CEACAM5 recombinant protein at 0.02 µg

Lane 3:

His-tagged human CEACAM6 recombinant protein at 0.02 µg

Lane 4:

His-tagged human CEACAM8 recombinant protein at 0.02 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 58 kDa

false

Exposure time: 48s

Western blot - Anti-CEACAM6 antibody [EPR4403] (AB134074)
  • WB

Unknown

Western blot - Anti-CEACAM6 antibody [EPR4403] (AB134074)

All lanes:

Western blot - Anti-CEACAM6 antibody [EPR4403] (ab134074) at 1/10000 dilution

Lane 1:

Human placenta cell lysate at 10 µg

Lane 2:

JAR cell lysate at 10 µg

Lane 3:

HT29 cell lysate at 10 µg

Lane 4:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4403

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/50000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEACAM6 also known as 9a6 CEACAM-6 and 1h7 is a cell surface glycoprotein weighing approximately 35-40 kDa. The protein plays a mechanical role as an intercellular adhesion molecule. It displays broad expression across multiple tissues with high levels found in epithelial cells of the gastrointestinal tract respiratory tract and in leukocytes.
Biological function summary

CEACAM6 acts in cell signaling and adhesion processes. It often forms complexes with other members of the CEACAM family such as CEACAM5. This protein is involved in the regulation of cell-cell adhesion which impacts cell migration and organization. It also influences immune reactions by modulating the interactions between pathogens and host cells.

Pathways

CEACAM6 integrates into pathways associated with cell adhesion and immune regulation. It engages in the PI3K-Akt signaling pathway tightly interacting with proteins such as CEACAM1 and CEACAM5. Through these interactions CEACAM6 plays an essential role in cell survival proliferation and immune responses.

CEACAM6 shows significant links with cancer especially colorectal and pancreatic cancers. Overexpression of this protein correlates with tumor progression and metastasis. Additionally in inflammatory bowel disease CEACAM6 association with CEACAM7 often results in altered epithelial barrier function contributing to disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface glycoprotein that plays a role in cell adhesion and tumor progression (PubMed : 10910050, PubMed : 11590190, PubMed : 1378450, PubMed : 16204051, PubMed : 2022629, PubMed : 2803308, PubMed : 8776764). Intercellular adhesion occurs in a calcium- and fibronectin-independent manner (PubMed : 16204051, PubMed : 2022629). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8 (PubMed : 11590190, PubMed : 16204051, PubMed : 2022629, PubMed : 2803308, PubMed : 8776764). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed : 8776764). Plays a role in neutrophil adhesion to cytokine-activated endothelial cells (PubMed : 1378450). Plays a role in cell migration and cell adhesion to endothelial cells (PubMed : 16204051).
See full target information CEACAM6
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Genome biology 23:106 PubMed35484598

2022

Systematic evaluation of colorectal cancer organoid system by single-cell RNA-Seq analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Wang,Yunuo Mao,Wendong Wang,Xin Zhou,Wei Wang,Shuai Gao,Jingyun Li,Lu Wen,Wei Fu,Fuchou Tang

Cancer cell international 21:549 PubMed34663338

2021

Identification of key genes for HNSCC from public databases using bioinformatics analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuchu Ye,Jingyi Wang,Faya Liang,Pan Song,Xiaoqing Yan,Sangqing Wu,Xiaoming Huang,Ping Han

Oncology letters 20:2677-2688 PubMed32782584

2020

Effect of silencing on the biological behavior of human gallbladder cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chengming Tian,Bingye Zhang,Chunlin Ge

Experimental cell research 378:206-216 PubMed30772380

2019

A SUV39H1-low chromatin state characterises and promotes migratory properties of cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Calvin Rodrigues,Chitra Pattabiraman,Anjali Vijaykumar,Reety Arora,Suma Mysore Narayana,Rekha V Kumar,Dimple Notani,Patrick Varga-Weisz,Sudhir Krishna

Oncology letters 13:3753-3759 PubMed28521477

2017

T cell immunity induced by a bivalent Salmonella-based CEACAM6 and 4-1BBL vaccines in a rat colorectal cancer model.

Applications

Unspecified application

Species

Unspecified reactive species

Chunhui Jin,Xiaoqing Duan,Yingying Liu,Jianhong Zhu,Ke Zhang,Yuanting Zhang,Tingting Xia,Yajun Fei,Jianxin Ye

Oncology letters 11:3693-3701 PubMed27284373

2016

FOXP3 and CEACAM6 expression and T cell infiltration in the occurrence and development of colon cancer.

Applications

IHC

Species

Unspecified reactive species

Yingying Liu,Tingting Xia,Chunhui Jin,Dongmei Gu,Jie Yu,Weiqiang Shi,K E Zhang,Liping Zhang,Jianxin Ye,Ling Li
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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