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AB248662

Anti-CEACAM6 antibody [EPR4404(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal CEAM6 antibody. Carrier free. Suitable for WB and reacts with Recombinant full length protein - Human, Human samples.

View Alternative Names

CD66c, NCA, CEACAM6, Cell adhesion molecule CEACAM6, Carcinoembryonic antigen-related cell adhesion molecule 6, Non-specific crossreacting antigen, Normal cross-reacting antigen, CEA cell adhesion molecule 6

2 Images
Western blot - Anti-CEACAM6 antibody [EPR4404(2)] - BSA and Azide free (AB248662)
  • WB

Unknown

Western blot - Anti-CEACAM6 antibody [EPR4404(2)] - BSA and Azide free (AB248662)

This data was developed using ab134058, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CEACAM6 antibody [EPR4404(2)] (<a href='/en-us/products/primary-antibodies/ceacam6-antibody-epr44042-ab134058'>ab134058</a>) at 1/10000 dilution

Lane 1:

MCF-7 cell lysate at 10 µg

Lane 2:

SW480 cell lysate at 10 µg

Lane 3:

HT-29 cell lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Lane 5:

BxPC-3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa

false

Western blot - Anti-CEACAM6 antibody [EPR4404(2)] - BSA and Azide free (AB248662)
  • WB

Lab

Western blot - Anti-CEACAM6 antibody [EPR4404(2)] - BSA and Azide free (AB248662)

All lanes : Anti-CEACAM6 antibody [EPR4404(2)] (ab134058) at 1/1000 dilution Lane 1 : His-tagged human CEACAM1 recombinant protein (aa 35-428) Lane 2 : His-tagged human CEACAM3 recombinant protein (aa 35-252) Lane 3 : His-tagged human CEACAM5 recombinant protein (aa 35-586) Lane 4 : His-tagged human CEACAM6 recombinant protein (aa 35-320) Lane 5 : His-tagged human CEACAM8 recombinant protein (aa 35-320) Lysates/proteins at 20 ng per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution Observed band size : 60 kDa Exposure time : 3 minute Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human CEACAM1, CEACAM3, CEACAM5, or CEACAM8. This data was developed using ab134058, the same antibody clone in a different buffer formulation.

Lanes 1 - 5:

Western blot - Anti-CEACAM6 antibody [EPR4404(2)] - BSA and Azide free (ab248662) at 1/1000 dilution

Lanes 1 - 5:

Western blot - Anti-CEACAM6 antibody [EPR4404(2)] (<a href='/en-us/products/primary-antibodies/ceacam6-antibody-epr44042-ab134058'>ab134058</a>) at 1/1000 dilution

Lane 1:

His-tagged human CEACAM1 recombinant protein (aa 35-428) at 20 ng

Lane 2:

His-tagged human CEACAM3 recombinant protein (aa 35-252) at 20 ng

Lane 3:

His-tagged human CEACAM5 recombinant protein (aa 35-586) at 20 ng

Lane 4:

His-tagged human CEACAM6 recombinant protein (aa 35-320) at 20 ng

Lane 5:

His-tagged human CEACAM8 recombinant protein (aa 35-320) at 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 37 kDa

Observed band size: 60 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4404(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Transfected cell line - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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AB283545

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEACAM6 also known as 9a6 CEACAM-6 and 1h7 is a cell surface glycoprotein weighing approximately 35-40 kDa. The protein plays a mechanical role as an intercellular adhesion molecule. It displays broad expression across multiple tissues with high levels found in epithelial cells of the gastrointestinal tract respiratory tract and in leukocytes.
Biological function summary

CEACAM6 acts in cell signaling and adhesion processes. It often forms complexes with other members of the CEACAM family such as CEACAM5. This protein is involved in the regulation of cell-cell adhesion which impacts cell migration and organization. It also influences immune reactions by modulating the interactions between pathogens and host cells.

Pathways

CEACAM6 integrates into pathways associated with cell adhesion and immune regulation. It engages in the PI3K-Akt signaling pathway tightly interacting with proteins such as CEACAM1 and CEACAM5. Through these interactions CEACAM6 plays an essential role in cell survival proliferation and immune responses.

CEACAM6 shows significant links with cancer especially colorectal and pancreatic cancers. Overexpression of this protein correlates with tumor progression and metastasis. Additionally in inflammatory bowel disease CEACAM6 association with CEACAM7 often results in altered epithelial barrier function contributing to disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface glycoprotein that plays a role in cell adhesion and tumor progression (PubMed : 10910050, PubMed : 11590190, PubMed : 1378450, PubMed : 16204051, PubMed : 2022629, PubMed : 2803308, PubMed : 8776764). Intercellular adhesion occurs in a calcium- and fibronectin-independent manner (PubMed : 16204051, PubMed : 2022629). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8 (PubMed : 11590190, PubMed : 16204051, PubMed : 2022629, PubMed : 2803308, PubMed : 8776764). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed : 8776764). Plays a role in neutrophil adhesion to cytokine-activated endothelial cells (PubMed : 1378450). Plays a role in cell migration and cell adhesion to endothelial cells (PubMed : 16204051).
See full target information CEACAM6
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

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Alternative Product
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