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Rabbit Recombinant Monoclonal CEBP Alpha/CEBPA antibody. Suitable for ChIC/CUT&RUN-seq, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 19 publications.


Images

Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (AB40761), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (AB40761), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (AB40761), expandable thumbnail
  • Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (AB40761), expandable thumbnail
  • ChIC/CUT&RUN sequencing - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (AB40761), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIC/CUT&RUN-seqIPIHCWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Not recommended
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

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Target data

Function

Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' acting as an activator on distinct target genes (PubMed:11242107). During early embryogenesis, plays essential and redundant functions with CEBPB. Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP). Critical for the proper development of the liver and the lung (By similarity). Necessary for terminal adipocyte differentiation, is required for postnatal maintenance of systemic energy homeostasis and lipid storage (By similarity). To regulate these different processes at the proper moment and tissue, interplays with other transcription factors and modulators. Down-regulates the expression of genes that maintain cells in an undifferentiated and proliferative state through E2F1 repression, which is critical for its ability to induce adipocyte and granulocyte terminal differentiation. Reciprocally E2F1 blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. Proliferation arrest also depends on a functional binding to SWI/SNF complex (PubMed:14660596). In liver, regulates gluconeogenesis and lipogenesis through different mechanisms. To regulate gluconeogenesis, functionally cooperates with FOXO1 binding to IRE-controlled promoters and regulating the expression of target genes such as PCK1 or G6PC1. To modulate lipogenesis, interacts and transcriptionally synergizes with SREBF1 in promoter activation of specific lipogenic target genes such as ACAS2. In adipose tissue, seems to act as FOXO1 coactivator accessing to ADIPOQ promoter through FOXO1 binding sites (By similarity). Isoform 3. Can act as dominant-negative. Binds DNA and have transctivation activity, even if much less efficiently than isoform 2. Does not inhibit cell proliferation (PubMed:14660596). Isoform 4. Directly and specifically enhances ribosomal DNA transcription interacting with RNA polymerase I-specific cofactors and inducing histone acetylation.

Alternative names

CEBP, CEBPA, CCAAT/enhancer-binding protein alpha, C/EBP alpha

Recommended products

Rabbit Recombinant Monoclonal CEBP Alpha/CEBPA antibody. Suitable for ChIC/CUT&RUN-seq, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 19 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP708Y
Purification technique
Affinity purification Protein A
Specificity

This antibody recognizes isoform1 and isoform 4.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CEBP Alpha also known as CEBPA is a transcription factor belonging to the CCAAT/enhancer-binding protein family. It is composed of a molecular mass approximately between 42 to 45 kDa and is characterized by the leucine zipper motif which contributes to its function in dimerization and DNA binding. CEBP Alpha is expressed in various tissues with notable expression in the liver adipose tissue and hematopoietic cells where it plays a significant role in regulating gene expression related to growth arrest and differentiation.

Biological function summary

CEBP Alpha regulates important cellular processes by acting as a transcriptional regulator. It participates in the control of cellular differentiation proliferation and metabolism. CEBP Alpha forms homo- or heterodimers with other proteins of the CEBP family to exert its functions. It induces expression of genes involved in differentiation of myeloid progenitors into granulocytes and macrophages. The protein also promotes adipogenesis by activating genes responsible for the development and function of adipocytes.

Pathways

CEBP Alpha participates actively in signaling pathways that involve cell cycle control and hematopoiesis. In the hematopoietic pathway CEBP Alpha influences the differentiation of progenitor cells into mature blood cells. Additionally it interacts with other proteins such as PU.1 an important regulator in the myeloid lineage and through the MAPK pathway influences growth arrest and differentiation responses. These interactions highlight its essential role in maintaining the balance between cell proliferation and differentiation.

Associated diseases and disorders

Alterations in the function or expression of CEBP Alpha have consequential links to acute myeloid leukemia (AML) and obesity. Mutations or disruptions in the CEBP Alpha gene can lead to impaired granulocyte differentiation contributing to the pathogenesis of AML. Additionally CEBP Alpha through its role in adipogenesis is associated with metabolic disorders such as obesity. The protein's interaction with other transcription factors like FLT3 and GATA-2 in the context of these diseases highlights its critical involvement in maintaining normal physiological functions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761), expandable thumbnail

    Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)

    We are unsure as to the identity of the 30kda band.

    All lanes: Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761) at 1/1000 dilution

    All lanes: U937 + LPS cell lysate at 10 µg

    Predicted band size: 38 kDa

    Observed band size: 30 kDa, 43 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)

    ab40761 (1/250) staining HeLa cells using immunofluorescence.

  • Flow Cytometry (Intracellular) - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)

    Overlay histogram showing HeLa cells stained with ab40761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40761, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)

    Blocked with 5% Milk for 1 hour at 24°C.

    We are unsure as to the identity of the 30kda band.

    All lanes: Western blot - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761) at 1/5000 dilution

    All lanes: Human MCF-7 whole cell lysate at 100000 Cells

    Secondary

    All lanes: HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa

    Observed band size: 40 kDa

    Exposure time: 1min

  • ChIC/CUT&RUN sequencing - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 THP-1 (human monocytic leukemia monocyte) cells and 5 µg of ab40761 [EP708Y]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.

    The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) method

  • ChIC/CUT&RUN sequencing - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 THP-1 (human monocytic leukemia monocyte) cells and 5 µg of ab40761 [EP708Y]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.

    The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

  • ChIC/CUT&RUN sequencing - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-CEBP Alpha/CEBPA antibody [EP708Y] (ab40761)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 THP-1 (human monocytic leukemia monocyte) cells and 5 µg of ab40761 [EP708Y]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.

    The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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