JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317443

Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free

  • BOND RX™ Validated
  • Recombinant
  • Advanced Validation
  • RabMAb
  • What is this?

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal CEBP Alpha/CEBPA antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, Flow Cyt (Intra), IHC-P, WB, IP, ChIP-seq and reacts with Human, Mouse, Rat samples.

View Alternative Names

CEBP, CEBPA, CCAAT/enhancer-binding protein alpha, C/EBP alpha

17 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)

This data was developed using ab317442, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CEBP Alpha/CEBPA with ab317442 at 1/500 (1.004 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human tonsil. The section was incubated with ab317442 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Flow Cytometry (Intracellular) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)

This data was developed using ab317442, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (human monocytic leukemia monocyte) (Right) / Jurkat (human T cell leukemia T lymphocyte from peripheral blood) (Left) cells labelling CEBP Alpha/CEBPA with ab317442 at 1/5000 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Low expression : Jurkat.

ChIP-sequencing - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)
  • ChIP-seq

Supplier Data

ChIP-sequencing - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)

This data was developed using ab317442, the same antibody clone in a different buffer formulation.

Chromatin was prepared from THP-1 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab317442 [EPR27924-90]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 60 million reads. The Input control is also shown.

ChIP-sequencing - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)
  • ChIP-seq

Supplier Data

ChIP-sequencing - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)

This data was developed using ab317442, the same antibody clone in a different buffer formulation.

Chromatin was prepared from THP-1 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab317442 [EPR27924-90]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 60 million reads. The Input control is also shown.

ChIP-sequencing - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)
  • ChIP-seq

Supplier Data

ChIP-sequencing - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)

This data was developed using ab317442, the same antibody clone in a different buffer formulation.

Chromatin was prepared from THP-1 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab317442 [EPR27924-90]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 60 million reads. The Input control is also shown.

Immunoprecipitation - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)
  • IP

Supplier Data

Immunoprecipitation - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)

This data was developed using ab317442, the same antibody clone in a different buffer formulation.

CEBP Alpha/CEBPA was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate with ab317442 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317442 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 2 : ab317442 IP in THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317442 in THP-1 whole cell lysate

Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (<a href='/en-us/products/primary-antibodies/cebp-alpha-cebpa-antibody-epr27924-90-ab317442'>ab317442</a>) at 1/30 dilution

All lanes:

THP-1 (human monocytic leukemia monocyte) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 6s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)

This data was developed using ab317442, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CEBP Alpha/CEBPA with ab317442 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse spleen. The section was incubated with ab317442 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)

This data was developed using ab317442, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CEBP Alpha/CEBPA with ab317442 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat spleen. The section was incubated with ab317442 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)

This data was developed using ab317442, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling CEBP Alpha/CEBPA with ab317442 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat liver. The section was incubated with ab317442 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)

This data was developed using ab317442, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CEBP Alpha/CEBPA with ab317442 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse liver (PMID : 2792758). The section was incubated with ab317442 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunoprecipitation - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)
  • IP

Supplier Data

Immunoprecipitation - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)

This data was developed using ab317442, the same antibody clone in a different buffer formulation.

CEBP Alpha/CEBPA was immunoprecipitated from 0.35 mg MEF (mouse embryo fibroblast) whole cell lysate with ab317442 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317442 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : MEF (mouse embryo fibroblast) whole cell lysate
Lane 2 : ab317442 IP in MEF (mouse embryo fibroblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317442 in MEF whole cell lysate

Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (<a href='/en-us/products/primary-antibodies/cebp-alpha-cebpa-antibody-epr27924-90-ab317442'>ab317442</a>) at 1/30 dilution

All lanes:

MEF (mouse embryo fibroblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

Western blot - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)
  • WB

Supplier Data

Western blot - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)

This data was developed using ab317442, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (<a href='/en-us/products/primary-antibodies/cebp-alpha-cebpa-antibody-epr27924-90-ab317442'>ab317442</a>) at 1/1000 dilution

Lane 1:

Human lung tissue lysate at 20 µg

Lane 2:

Human liver cancer tissue lysate at 20 µg

Lane 3:

Human tonsil tissue lysate at 20 µg

Lane 4:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 41 kDa,36 kDa

true

Exposure time: 70s

Western blot - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)
  • WB

Supplier Data

Western blot - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)

This data was developed using ab317442, the same antibody clone in a different buffer formulation.

Low expression : Jurkat.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8415748).

The lanes 5-6 were developed using a high sensitivity ECL substrate.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-4 : 26 seconds, lane 5 : 37 seconds, lane 6 : 180 seconds, lane 7 : 48 seconds

All lanes:

Western blot - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (<a href='/en-us/products/primary-antibodies/cebp-alpha-cebpa-antibody-epr27924-90-ab317442'>ab317442</a>) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

THP-1 transfected with siRNA specifically targeting CEBP Alpha/CEBPA whole cell lysate at 20 µg

Lane 3:

THP-1 whole cell lysate at 20 µg

Lane 4:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

Lane 5:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 6:

NR8383 (rat alveolar macrophage) whole cell lysate at 20 µg

Lane 7:

MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 26 kDa,36 kDa,41 kDa

false

ChIC/CUT&RUN sequencing - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)
  • ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)

This data was developed using ab317442, the same antibody clone in a different buffer formulation.

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 THP-1 (human monocytic leukemia monocyte) cells and 5 µg of ab317442 [EPR27924-90]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

ChIC/CUT&RUN sequencing - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)
  • ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)

This data was developed using ab317442, the same antibody clone in a different buffer formulation.

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 THP-1 (human monocytic leukemia monocyte) cells and 5 µg of ab317442 [EPR27924-90]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

ChIC/CUT&RUN sequencing - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)
  • ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)

This data was developed using ab317442, the same antibody clone in a different buffer formulation.

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 THP-1 (human monocytic leukemia monocyte) cells and 5 µg of ab317442 [EPR27924-90]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

Western blot - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)
  • WB

Supplier Data

Western blot - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] - BSA and Azide free (AB317443)

This data was developed using ab317442, the same antibody clone in a different buffer formulation.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8415748).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CEBP Alpha/CEBPA antibody [EPR27924-90] (<a href='/en-us/products/primary-antibodies/cebp-alpha-cebpa-antibody-epr27924-90-ab317442'>ab317442</a>) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 41 kDa,36 kDa

false

Exposure time: 48s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27924-90

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IHC-P, ChIC/CUT&RUN-seq, ChIP-seq, IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB207199

C/EBP alpha/beta Transcription Factor Assay Kit (Colorimetric)

Functional Studies - C/EBP alpha/beta Transcription Factor Assay Kit (Colorimetric) (AB207199)

  • 0
  • 0
Primary Antibodies

AB32358

Anti-CEBP Beta antibody [E299] - C-terminal

RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-CEBP Beta antibody [E299] - C-terminal (AB32358)

  • 5
  • 7
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB272718

Anti-PPAR gamma antibody [EPR23297-111]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody [EPR23297-111] (AB272718)

  • 2
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "<p></p>" }, "Mouse": { "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" }, "Rat": { "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" } } }
style
left-column

Product details

ab317443 is the carrirer-free version of ab317442.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEBP Alpha also known as CEBPA is a transcription factor belonging to the CCAAT/enhancer-binding protein family. It is composed of a molecular mass approximately between 42 to 45 kDa and is characterized by the leucine zipper motif which contributes to its function in dimerization and DNA binding. CEBP Alpha is expressed in various tissues with notable expression in the liver adipose tissue and hematopoietic cells where it plays a significant role in regulating gene expression related to growth arrest and differentiation.
Biological function summary

CEBP Alpha regulates important cellular processes by acting as a transcriptional regulator. It participates in the control of cellular differentiation proliferation and metabolism. CEBP Alpha forms homo- or heterodimers with other proteins of the CEBP family to exert its functions. It induces expression of genes involved in differentiation of myeloid progenitors into granulocytes and macrophages. The protein also promotes adipogenesis by activating genes responsible for the development and function of adipocytes.

Pathways

CEBP Alpha participates actively in signaling pathways that involve cell cycle control and hematopoiesis. In the hematopoietic pathway CEBP Alpha influences the differentiation of progenitor cells into mature blood cells. Additionally it interacts with other proteins such as PU.1 an important regulator in the myeloid lineage and through the MAPK pathway influences growth arrest and differentiation responses. These interactions highlight its essential role in maintaining the balance between cell proliferation and differentiation.

Alterations in the function or expression of CEBP Alpha have consequential links to acute myeloid leukemia (AML) and obesity. Mutations or disruptions in the CEBP Alpha gene can lead to impaired granulocyte differentiation contributing to the pathogenesis of AML. Additionally CEBP Alpha through its role in adipogenesis is associated with metabolic disorders such as obesity. The protein's interaction with other transcription factors like FLT3 and GATA-2 in the context of these diseases highlights its critical involvement in maintaining normal physiological functions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' acting as an activator on distinct target genes (PubMed : 11242107). During early embryogenesis, plays essential and redundant functions with CEBPB. Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP). Critical for the proper development of the liver and the lung (By similarity). Necessary for terminal adipocyte differentiation, is required for postnatal maintenance of systemic energy homeostasis and lipid storage (By similarity). To regulate these different processes at the proper moment and tissue, interplays with other transcription factors and modulators. Down-regulates the expression of genes that maintain cells in an undifferentiated and proliferative state through E2F1 repression, which is critical for its ability to induce adipocyte and granulocyte terminal differentiation. Reciprocally E2F1 blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. Proliferation arrest also depends on a functional binding to SWI/SNF complex (PubMed : 14660596). In liver, regulates gluconeogenesis and lipogenesis through different mechanisms. To regulate gluconeogenesis, functionally cooperates with FOXO1 binding to IRE-controlled promoters and regulating the expression of target genes such as PCK1 or G6PC1. To modulate lipogenesis, interacts and transcriptionally synergizes with SREBF1 in promoter activation of specific lipogenic target genes such as ACAS2. In adipose tissue, seems to act as FOXO1 coactivator accessing to ADIPOQ promoter through FOXO1 binding sites (By similarity).. Isoform 3. Can act as dominant-negative. Binds DNA and have transctivation activity, even if much less efficiently than isoform 2. Does not inhibit cell proliferation (PubMed : 14660596).. Isoform 4. Directly and specifically enhances ribosomal DNA transcription interacting with RNA polymerase I-specific cofactors and inducing histone acetylation.
See full target information CEBPA
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com