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AB245214

Anti-CEBP Delta/CEBPD antibody [EPR23518-259]

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(4 Publications)

Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214) is a rabbit monoclonal antibody detecting CEBP Delta/CEBPD in Western Blot, IP, IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CCAAT/enhancer-binding protein delta, C/EBP delta, Nuclear factor NF-IL6-beta, NF-IL6-beta, CEBPD

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (AB245214)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (AB245214)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling CEBP Delta/CEBPD with ab245214 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and weak cytoplasmic staining on rat cerebrum is observed. The section was incubated with ab245214 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Western blot - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (AB245214)
  • WB

Lab

Western blot - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (AB245214)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Western blot - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214) at 1/1000 dilution

Lane 1:

Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HaCaT (human skin keratinocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 28 kDa

Observed band size: 29 kDa

false

Exposure time: 180s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (AB245214)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (AB245214)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse hippocampus is observed. The section was incubated with ab245214 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (AB245214)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (AB245214)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue). Nuclear staining on mouse hippocampus is observed.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (AB245214)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (AB245214)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue). Nuclear staining on rat hippocampus is observed.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

Immunoprecipitation - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (AB245214)
  • IP

Lab

Immunoprecipitation - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (AB245214)

CEBP Delta/CEBPD was immunoprecipitated from 0.35 mg differentiated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate with ab245214 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 245214 1/1000 dilution (0.518 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Differentiated 3T3-L1 (mouse embryonic fibroblast), whole cell lysate 10 μg

Lane 2 : ab245214 IP in Differentiated 3T3-L1 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab245214 in differentiated 3T3-L1 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

Predicted band size: 28 kDa

Observed band size: 29 kDa

false

Western blot - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (AB245214)
  • WB

Lab

Western blot - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (AB245214)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Western blot - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214) at 1/1000 dilution

All lanes:

Differentiated 3T3-L1 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 28 kDa

Observed band size: 29 kDa

false

Exposure time: 180s

  • Carrier free

    Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23518-259

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, IP, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, Rat samples.

What is the molecular weight of CEBP Delta/CEBPD?
Anti-CEBP Delta/CEBPD [EPR23518-259] (ab245214) specifically detects a band for CEBP Delta/CEBPD (UniProt: Q00322) at a molecular weight of 29kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR23518-259] also available for your convenience: ab245214, Carrier free - ab270410

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEBP Delta also known as CEBPD is a transcription factor belonging to the CCAAT/enhancer-binding protein family. This protein has a molecular mass of approximately 28 kDa. It is expressed in various tissues including liver adipose tissue and the immune system reflecting its involvement in distinct physiological processes. CEBPD contains a basic leucine zipper domain allowing it to bind to specific DNA sequences. It acts as a regulator of gene expression by influencing transcriptional activity thereby playing a significant role in controlling cellular differentiation and metabolic processes.
Biological function summary

CEBP Delta participates in multiple cellular functions including cell cycle regulation and immune response. It modulates proliferation differentiation and apoptosis being important for normal and stress-induced cellular functions. CEBPD forms homodimers or heterodimers with other members of the CEBP family enhancing its functional diversity. This dimerization allows CEBPD to adapt its regulatory role depending on the specific cellular environment and stimuli contributing to metabolic homeostasis and immune functions.

Pathways

CEBP Delta plays a role in pathways related to inflammatory responses and metabolic regulation. It is involved in the NF-κB pathway which is critical for the immune system's response to infection and inflammation working alongside proteins like RELA and NFKB1. Additionally it affects metabolic pathways by regulating genes responsible for lipid and glucose metabolism an action interconnected with other transcription factors like PPARs. Through these pathways CEBPD influences cellular responses to stress and energy requirements.

CEBP Delta has been implicated in cancer development and inflammatory diseases. Its dysregulation can contribute to tumorigenesis as CEBPD affects cell proliferation and apoptosis processes often disrupted in cancer. Research suggests a connection between CEBPD and the tumor-suppressing protein p53 indicating a possible regulatory relationship significant in cancer pathways. Furthermore abnormal expression of CEBPD associates with inflammatory disorders such as rheumatoid arthritis where it intersects with cytokines impacting the inflammatory response and joint degradation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription activator that recognizes two different DNA motifs : the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers (PubMed : 16397300). Important transcription factor regulating the expression of genes involved in immune and inflammatory responses (PubMed : 16397300, PubMed : 1741402). Transcriptional activator that enhances IL6 transcription alone and as heterodimer with CEBPB (PubMed : 1741402).
See full target information CEBPD
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancers 15: PubMed37958388

2023

The Mechanism of Oxymatrine Targeting miR-27a-3p/PPAR-γ Signaling Pathway through m6A Modification to Regulate the Influence on Hemangioma Stem Cells on Propranolol Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxin Dai,Mingke Qiu,Shenglai Zhang,Jingyu Peng,Xin Hou,Jie Liu,Feifei Li,Jingmin Ou

Cell death & disease 14:269 PubMed37059730

2023

CEBPD is a master transcriptional factor for hypoxia regulated proteins in glioblastoma and augments hypoxia induced invasion through extracellular matrix-integrin mediated EGFR/PI3K pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xing-Gang Mao,Xiao-Yan Xue,Rui Lv,Ang Ji,Ting-Yu Shi,Xiao-Yan Chen,Xiao-Fan Jiang,Xiang Zhang

Cell 184:5357-5374.e22 PubMed34582788

2021

In vivo CRISPR screens identify the E3 ligase Cop1 as a modulator of macrophage infiltration and cancer immunotherapy target.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqing Wang,Collin Tokheim,Shengqing Stan Gu,Binbin Wang,Qin Tang,Yihao Li,Nicole Traugh,Zexian Zeng,Yi Zhang,Ziyi Li,Boning Zhang,Jingxin Fu,Tengfei Xiao,Wei Li,Clifford A Meyer,Jun Chu,Peng Jiang,Paloma Cejas,Klothilda Lim,Henry Long,Myles Brown,X Shirley Liu

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 70:591-603 PubMed33891136

2021

FOXO3a regulates lipid accumulation and adipocyte inflammation in adipocytes through autophagy : Role of FOXO3a in obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Zhang,Qiang Liu,Xuane Zhang,Kai Guo,Xuelian Zhang,Zunhai Zhou
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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