Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (AB270410)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse hippocampus is observed. The section was incubated with ab245214 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245214).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (AB270410)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/100 dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue). Nuclear staining on rat hippocampus is observed.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245214).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (AB270410)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/100 dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue). Nuclear staining on mouse hippocampus is observed.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245214).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (AB270410)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling CEBP Delta/CEBPD with ab245214 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and weak cytoplasmic staining on rat cerebrum is observed. The section was incubated with ab245214 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245214).

• IP

Lab

Immunoprecipitation - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (AB270410)

CEBP Delta/CEBPD was immunoprecipitated from 0.35 mg differentiated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate with ab245214 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 245214 1/1000 dilution (0.518 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Differentiated 3T3-L1 (mouse embryonic fibroblast), whole cell lysate 10 μg

Lane 2 : ab245214 IP in Differentiated 3T3-L1 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab245214 in differentiated 3T3-L1 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245214).

All lanes:

Immunoprecipitation - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (<a href='/en-us/products/primary-antibodies/cebp-delta-cebpd-antibody-epr23518-259-ab245214'>ab245214</a>)

Predicted band size: 28 kDa

Observed band size: 29 kDa

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