Rabbit Polyclonal CEECAM1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CERCAM.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CERCAM. The exact immunogen used to generate this antibody is proprietary information. Database link Q5T4B2
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
| Rat | Expected | Tested |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Probable cell adhesion protein involved in leukocyte transmigration across the blood-brain barrier. Does not express any beta-galactosyltransferase activity in vitro.
Alternative names
CEECAM1, GLT25D3, KIAA1502, CERCAM, Inactive glycosyltransferase 25 family member 3, Cerebral endothelial cell adhesion molecule
Recommended products
Rabbit Polyclonal CEECAM1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CERCAM.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CERCAM. The exact immunogen used to generate this antibody is proprietary information. Database link Q5T4B2
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CEECAM1 also known as Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 has a molecular mass of approximately 37-45 kDa. This glycoprotein belongs to the immunoglobulin superfamily and facilitates cell adhesion processes. CEECAM1 is mainly expressed in epithelial cells of several tissues including the gastrointestinal tract liver and lungs. It plays roles in cellular adhesion and signaling which are important for maintaining structural integrity and communication between cells.
- Biological function summary
CEECAM1 participates in modulating immune responses and cellular proliferation. This protein interacts with other molecules at the cell surface to form complexes that influence cell signaling pathways. CEECAM1's involvement in processes such as cell adhesion and migration makes it significant in tissue architecture and homeostasis. Through these functions CEECAM1 has an influence on both normal physiological processes and pathological conditions.
- Pathways
CEECAM1 is actively involved in the MAPK and PI3K/AKT signaling pathways. These pathways are critical for transmitting signals related to growth survival and differentiation of cells. In the MAPK pathway CEECAM1 interacts with proteins like ERK which regulates various cellular activities. Within the PI3K/AKT pathway CEECAM1 can influence activity impacting cell cycle progression and apoptosis. Its interactions within these pathways link CEECAM1 to a broader network of cellular functions.
- Associated diseases and disorders
CEECAM1 has associations with cancer progression and inflammatory diseases. In various cancers such as colorectal cancer CEECAM1 expression levels can change influencing tumor progression and metastasis. It often interacts with proteins like CEA (Carcinoembryonic Antigen) which is involved in tumorigenesis. In inflammatory diseases CEECAM1's role in modulating immune responses can alter inflammatory pathways affecting tissue damage and repair mechanisms. Understanding CEECAM1's role in these diseases continues to be an area of active research.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-CEECAM1 antibody (ab227943)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-CEECAM1 antibody (ab227943) at 1/500 dilution
All lanes: Mouse brain lysate at 50 µg
Predicted band size: 68 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEECAM1 antibody (ab227943)
Paraffin-embedded rat liver tissue stained for CEECAM1 using ab227943 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-CEECAM1 antibody (ab227943)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-CEECAM1 antibody (ab227943) at 1/500 dilution
Lane 1: GL261 whole cell lysate at 30 µg
Lane 2: C8D30 whole cell lysate at 30 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 68 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEECAM1 antibody (ab227943)
Paraffin-embedded human hepatoma tissue stained for CEECAM1 using ab227943 at 1/500 dilution in immunohistochemical analysis.
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