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AB250547

Anti-CEECAM1 antibody [EPR13744] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CEECAM1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CEECAM1, GLT25D3, KIAA1502, CERCAM, Inactive glycosyltransferase 25 family member 3, Cerebral endothelial cell adhesion molecule

2 Images
Flow Cytometry (Intracellular) - Anti-CEECAM1 antibody [EPR13744] - BSA and Azide free (AB250547)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CEECAM1 antibody [EPR13744] - BSA and Azide free (AB250547)

This data was developed using ab181979, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling CEECAM1 with ab181979 at 1/300 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (greeen), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Western blot - Anti-CEECAM1 antibody [EPR13744] - BSA and Azide free (AB250547)
  • WB

Supplier Data

Western blot - Anti-CEECAM1 antibody [EPR13744] - BSA and Azide free (AB250547)

This data was developed using ab181979, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CEECAM1 antibody [EPR13744] (<a href='/en-us/products/primary-antibodies/ceecam1-antibody-epr13744-ab181979'>ab181979</a>) at 1/10000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Lane 4:

U87-MG cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13744

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250547 is the carrier-free version of ab181979.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEECAM1 also known as Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 has a molecular mass of approximately 37-45 kDa. This glycoprotein belongs to the immunoglobulin superfamily and facilitates cell adhesion processes. CEECAM1 is mainly expressed in epithelial cells of several tissues including the gastrointestinal tract liver and lungs. It plays roles in cellular adhesion and signaling which are important for maintaining structural integrity and communication between cells.
Biological function summary

CEECAM1 participates in modulating immune responses and cellular proliferation. This protein interacts with other molecules at the cell surface to form complexes that influence cell signaling pathways. CEECAM1's involvement in processes such as cell adhesion and migration makes it significant in tissue architecture and homeostasis. Through these functions CEECAM1 has an influence on both normal physiological processes and pathological conditions.

Pathways

CEECAM1 is actively involved in the MAPK and PI3K/AKT signaling pathways. These pathways are critical for transmitting signals related to growth survival and differentiation of cells. In the MAPK pathway CEECAM1 interacts with proteins like ERK which regulates various cellular activities. Within the PI3K/AKT pathway CEECAM1 can influence activity impacting cell cycle progression and apoptosis. Its interactions within these pathways link CEECAM1 to a broader network of cellular functions.

CEECAM1 has associations with cancer progression and inflammatory diseases. In various cancers such as colorectal cancer CEECAM1 expression levels can change influencing tumor progression and metastasis. It often interacts with proteins like CEA (Carcinoembryonic Antigen) which is involved in tumorigenesis. In inflammatory diseases CEECAM1's role in modulating immune responses can alter inflammatory pathways affecting tissue damage and repair mechanisms. Understanding CEECAM1's role in these diseases continues to be an area of active research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable cell adhesion protein involved in leukocyte transmigration across the blood-brain barrier. Does not express any beta-galactosyltransferase activity in vitro.
See full target information CERCAM
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:458 PubMed37736555

2023

S100A6 is a potential diagnostic and prognostic biomarker for human glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hong,Hui Zhang,Yufei Xiao,Lingwei Shen,Yun Qian
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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