JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB121249

Anti-CELF3 antibody

5

(1 Review)

|

(0 Publication)

Rabbit Polyclonal CELF3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CELF3 aa 250-450.

View Alternative Names

BRUNOL1, CAGH4, ERDA4, TNRC4, CELF3, CUGBP Elav-like family member 3, CELF-3, Bruno-like protein 1, CAG repeat protein 4, CUG-BP- and ETR-3-like factor 3, ELAV-type RNA-binding protein 1, Expanded repeat domain protein CAG/CTG 4, RNA-binding protein BRUNOL-1, Trinucleotide repeat-containing gene 4 protein, ETR-1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CELF3 antibody (AB121249)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CELF3 antibody (AB121249)

Immunohistochemical staining of paraffin embedded Human cerebral cortex, using 1/35 ab121249, shows distinct cytoplasmic positivity in neurons.

Immunocytochemistry/ Immunofluorescence - Anti-CELF3 antibody (AB121249)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CELF3 antibody (AB121249)

Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate labeling CELF3 with ab121249 at 2 μg/mL.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CELF3 aa 250-450. The exact immunogen used to generate this antibody is proprietary information.

Q5SZQ8

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p>Use buffer at pH 6 for antigen retrieval.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CELF3 protein also known as CUGBP Elav-like family member 3 belongs to the CELF family of RNA-binding proteins. With a molecular weight of approximately 55 kDa CELF3 localizes mainly in the brain and nervous tissue. It functions by binding to RNA which affects the stability and translation of the RNA molecules. This protein can also regulate alternative splicing contributing to the processing diversity of pre-mRNA molecules within cells.
Biological function summary

RNA-binding proteins such as CELF3 play significant roles in various cellular processes including gene expression regulation and maintaining RNA stability. CELF3 is not part of a larger complex but interacts with different RNA targets to influence their splicing patterns. In the nervous system CELF3 contributes to neuronal development and synaptic function impacting the fine-tuning of gene expression required for these processes.

Pathways

The RNA-binding activity of CELF3 impacts the mRNA processing pathway which is essential for mRNA maturation and function. It shows considerable overlap with other CELF family proteins like CELF1 and CELF2 that also partake in post-transcriptional regulation. CELF3’s role in alternative splicing also intertwines with the Wnt signaling pathway a pathway important for neuronal differentiation and development highlighting its regulatory importance in neurogenesis.

Aberrant expression or mutations of CELF3 correlates with neurological conditions such as epilepsy and neurodegenerative diseases. These associations manifest through altered RNA processing implicating CELF family members like CELF1 in these pathologies. CELF3’s involvement in splicing regulation further connects it to muscular dystrophy as misregulation may lead to improper muscle function seen in this disorder.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

RNA-binding protein involved in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA.
See full target information CELF3
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB117803

Anti-ZNF687 antibody

Immunoprecipitation - Anti-ZNF687 antibody (AB117803)

  • 0
  • 0
Primary Antibodies

AB118800

Anti-ZZZ3 antibody

Western blot - Anti-ZZZ3 antibody (AB118800)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB121685

Anti-ZNF750 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF750 antibody (AB121685)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com