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AB200657

Anti-Cellubrevin antibody [EPR16866]

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(4 Publications)

Rabbit Recombinant Monoclonal Cellubrevin antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

SYB3, VAMP3, Vesicle-associated membrane protein 3, VAMP-3, Cellubrevin, Synaptobrevin-3, CEB

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody [EPR16866] (AB200657)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody [EPR16866] (AB200657)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized A-375 (Human malignant melanoma cell line) cells labeling Cellubrevin with ab200657 at 1/250 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on A-375 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab200657 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody [EPR16866] (AB200657)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody [EPR16866] (AB200657)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling Cellubrevin with ab200657 at 1/250 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on A549 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab200657 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-Cellubrevin antibody [EPR16866] (AB200657)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cellubrevin antibody [EPR16866] (AB200657)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedA-375 (Human malignant melanoma cell line) cells labeling Cellubrevin with ab200657 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Immunoprecipitation - Anti-Cellubrevin antibody [EPR16866] (AB200657)
  • IP

Supplier Data

Immunoprecipitation - Anti-Cellubrevin antibody [EPR16866] (AB200657)

Cellubrevin was immunoprecipitated from 1mg of A-375 (Human malignant melanoma cell line) whole cell lysate with ab200657 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab200657 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1 : A-375 whole cell lysate 10μg (Input).
Lane 2 : ab200657 IP in A-375 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200657 in A-375 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-Cellubrevin antibody [EPR16866] (ab200657)

Predicted band size: 11 kDa

false

Western blot - Anti-Cellubrevin antibody [EPR16866] (AB200657)
  • WB

Supplier Data

Western blot - Anti-Cellubrevin antibody [EPR16866] (AB200657)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cellubrevin antibody [EPR16866] (ab200657) at 1/2000 dilution

All lanes:

Human fetal lung lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Exposure time: 30s

Western blot - Anti-Cellubrevin antibody [EPR16866] (AB200657)
  • WB

Supplier Data

Western blot - Anti-Cellubrevin antibody [EPR16866] (AB200657)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cellubrevin antibody [EPR16866] (ab200657) at 1/10000 dilution

All lanes:

A-375 (Human malignant melanoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Exposure time: 5s

Western blot - Anti-Cellubrevin antibody [EPR16866] (AB200657)
  • WB

Supplier Data

Western blot - Anti-Cellubrevin antibody [EPR16866] (AB200657)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cellubrevin antibody [EPR16866] (ab200657) at 1/10000 dilution

All lanes:

Human placenta tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Exposure time: 5s

  • Carrier free

    Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16866

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/60", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
See full target information VAMP3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy : the journal of the American Society of Gene Therapy 31:552-568 PubMed36245126

2022

HCP5 prevents ubiquitination-mediated UTP3 degradation to inhibit apoptosis by activating c-Myc transcriptional activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yabing Nan,Qingyu Luo,Xiaowei Wu,Wan Chang,Pengfei Zhao,Shi Liu,Zhihua Liu

eLife 9: PubMed32910773

2020

The host exosome pathway underpins biogenesis of the human cytomegalovirus virion.

Applications

Unspecified application

Species

Unspecified reactive species

Declan L Turner,Denis V Korneev,John G Purdy,Alex de Marco,Rommel A Mathias

ACS nano 13:13853-13865 PubMed31479239

2019

Tumor-Derived Extracellular Vesicles Breach the Intact Blood-Brain Barrier Transcytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Golnaz Morad,Christopher V Carman,Elliott J Hagedorn,Julie R Perlin,Leonard I Zon,Nur Mustafaoglu,Tae-Eun Park,Donald E Ingber,Cassandra C Daisy,Marsha A Moses

Cancer science 109:2199-2210 PubMed29788550

2018

Ubiquitin-specific protease 22 acts as an oncoprotein to maintain glioma malignancy through deubiquitinating B cell-specific Moloney murine leukemia virus integration site 1 for stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Guan-Zhong Qiu,Xiao-Yuan Mao,Yue Ma,Xing-Chun Gao,Zhen Wang,Ming-Zhu Jin,Wei Sun,Yong-Xiang Zou,Jing Lin,Hua-Lin Fu,Wei-Lin Jin
View all publications
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Product promise

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