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AB251315

Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free

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Rabbit Recombinant Monoclonal Cellubrevin antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

SYB3, VAMP3, Vesicle-associated membrane protein 3, VAMP-3, Cellubrevin, Synaptobrevin-3, CEB

7 Images
Western blot - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)
  • WB

Supplier Data

Western blot - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)

This data was developed using ab200657, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cellubrevin antibody [EPR16866] (<a href='/en-us/products/primary-antibodies/cellubrevin-antibody-epr16866-ab200657'>ab200657</a>) at 1/2000 dilution

All lanes:

Human fetal lung lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

false

Exposure time: 30s

Western blot - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)
  • WB

Supplier Data

Western blot - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)

This data was developed using ab200657, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cellubrevin antibody [EPR16866] (<a href='/en-us/products/primary-antibodies/cellubrevin-antibody-epr16866-ab200657'>ab200657</a>) at 1/10000 dilution

All lanes:

A-375 (Human malignant melanoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

false

Exposure time: 5s

Western blot - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)
  • WB

Supplier Data

Western blot - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)

This data was developed using ab200657, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cellubrevin antibody [EPR16866] (<a href='/en-us/products/primary-antibodies/cellubrevin-antibody-epr16866-ab200657'>ab200657</a>) at 1/10000 dilution

All lanes:

Human placenta tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

false

Exposure time: 5s

Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)

This data was developed using ab200657, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A-375 (Human malignant melanoma cell line) cells labeling Cellubrevin with ab200657 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on A-375 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab200657 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Immunoprecipitation - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)
  • IP

Supplier Data

Immunoprecipitation - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)

This data was developed using ab200657, the same antibody clone in a different buffer formulation.

Cellubrevin was immunoprecipitated from 1mg of A-375 (Human malignant melanoma cell line) whole cell lysate with ab200657 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab200657 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1 : A-375 whole cell lysate 10μg (Input).
Lane 2 : ab200657 IP in A-375 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200657 in A-375 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-Cellubrevin antibody [EPR16866] (<a href='/en-us/products/primary-antibodies/cellubrevin-antibody-epr16866-ab200657'>ab200657</a>)

false

Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)

This data was developed using ab200657, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling Cellubrevin with ab200657 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on A549 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab200657 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cellubrevin antibody [EPR16866] - BSA and Azide free (AB251315)

This data was developed using ab200657, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed A-375 (Human malignant melanoma cell line) cells labeling Cellubrevin with ab200657 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16866

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab251315 is the carrier-free version of ab200657.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
See full target information VAMP3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com