AB151546

Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Cellular Apoptosis Susceptibility/CSE1L antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CAS, XPO2, CSE1L, Exportin-2, Exp2, Cellular apoptosis susceptibility protein, Chromosome segregation 1-like protein, Importin-alpha re-exporter

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab151546 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

Immunofluorescent analysis of SW480 cells labeling Cellular Apoptosis Susceptibility/CSE1L with ab151546 at 1/250 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab151546 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab151546 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab151546 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling Cellular Apoptosis Susceptibility/CSE1L with ab151546 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using ab151546 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

False colour image of Western blot : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] staining at 1/1000 dilution, shown in black. In Western blot, ab151546 was shown to bind specifically to Cellular Apoptosis Susceptibility/CSE1L. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 2 minutes exposure time. Secondary antibodies used were VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution.

All lanes:

Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HeLa Pulldown with <a href='/en-us/products/primary-antibodies/cellular-apoptosis-susceptibility-cse1l-antibody-ab70547'>ab70547</a> cell lysate at 10 µL

Lane 3:

HeLa Pulldown with <a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> cell lysate at 10 µL

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/10000 dilution

Observed band size: 100 kDa

false

Unknown

Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

All lanes:

Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546) at 1/1000 dilution

Lane 1:

SW480 cell lysate at 10 µg

Lane 2:

MOLT4 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Lane 5:

Ramos cell lysate at 10 µg

Lane 6:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 110 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10327(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

See full target information CSE1L

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular & cellular proteomics : MCP 20:100115 PubMed34129943

2021

Age-Associated Proteomic Signatures and Potential Clinically Actionable Targets of Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanqiu Gong,Yu Liu,Tian Wang,Zhigui Li,Li Gao,Haining Chen,Yang Shu,Yuan Li,Heng Xu,Zongguang Zhou,Lunzhi Dai

Frontiers in microbiology 10:636 PubMed31001221

2019

Elucidating the Host Interactome of EV-A71 2C Reveals Viral Dependency Factors.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Li,Xia Jian,Peiqi Yin,Guofeng Zhu,Leiliang Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

Immunocytochemistry/ Immunofluorescence - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (2)

Product promise