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Rabbit Recombinant Monoclonal Cellular Apoptosis Susceptibility/CSE1L antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

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Images

Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (AB151546), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IF
Human
Tested
Not recommended
Tested
Tested
Mouse
Predicted
Not recommended
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/250 - 1/500

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

Alternative names

Exportin-2, Exp2, Cellular apoptosis susceptibility protein, Chromosome segregation 1-like protein, Importin-alpha re-exporter, XPO2, CAS, CSE1L

Recommended products

Rabbit Recombinant Monoclonal Cellular Apoptosis Susceptibility/CSE1L antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR10327(B)

Purity

Tissue culture supernatant

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546), expandable thumbnail

    Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546)

    All lanes: Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546) at 1/1000 dilution

    Lane 1: SW480 cell lysate at 10 µg

    Lane 2: MOLT4 cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Lane 4: 293T cell lysate at 10 µg

    Lane 5: Ramos cell lysate at 10 µg

    Lane 6: MCF7 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 110 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546)

    Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling Cellular Apoptosis Susceptibility/CSE1L with ab151546 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546)

    Immunofluorescent analysis of SW480 cells labeling Cellular Apoptosis Susceptibility/CSE1L with ab151546 at 1/250 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546)

    Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab151546 showing +ve staining.


    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546)

    Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using ab151546 showing +ve staining.


    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546)

    Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab151546 showing +ve staining.


    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546)

    Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab151546 showing +ve staining.


    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546)

    Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab151546 showing +ve staining.


    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546), expandable thumbnail

    Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546)

    False colour image of Western blot: Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] staining at 1/1000 dilution, shown in black. In Western blot, ab151546 was shown to bind specifically to Cellular Apoptosis Susceptibility/CSE1L. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent Anti-PKC delta (phospho S299) antibody [EPNCI119] ab133456) and imaged with 2 minutes exposure time. Secondary antibodies used were VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/10000 dilution.

    All lanes: Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: HeLa Pulldown with Anti-Cellular Apoptosis Susceptibility/CSE1L antibody ab70547 cell lysate at 10 µL

    Lane 3: HeLa Pulldown with Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 cell lysate at 10 µL

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size: 100 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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