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AB225889

Anti-CELSR1 antibody

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(2 Publications)

Rabbit Polyclonal CELSR1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CELSR1 aa 2750-2850.

View Alternative Names

CDHF9, FMI2, CELSR1, Cadherin EGF LAG seven-pass G-type receptor 1, Cadherin family member 9, Flamingo homolog 2, hFmi2

2 Images
Immunoprecipitation - Anti-CELSR1 antibody (AB225889)
  • IP

Supplier Data

Immunoprecipitation - Anti-CELSR1 antibody (AB225889)

CELSR1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN buffer; 1 mg for IP, 20% of IP loaded) with ab225889 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225889 at 0.4 μg/ml.

Lane 1 : ab225889 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-CELSR1 antibody (ab225889)

Predicted band size: 329 kDa

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Western blot - Anti-CELSR1 antibody (AB225889)
  • WB

Supplier Data

Western blot - Anti-CELSR1 antibody (AB225889)

Lysates prepared using NETN buffer.

All lanes:

Western blot - Anti-CELSR1 antibody (ab225889) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 329 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human CELSR1 aa 2750-2850. The exact immunogen used to generate this antibody is proprietary information.

Q9NYQ6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab225889 was affinity purified using an epitope specific to TRAF2 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CELSR1 also known as Cadherin EGF LAG seven-pass G-type receptor 1 is a member of the adhesion G-protein-coupled receptors family. Its molecular mass is approximately 350 kDa. The protein consists of several cadherin repeats and EGF-like domains playing a critical role in cell signaling and adhesion. CELSR1 is mainly expressed in the central nervous system and certain epithelial tissues contributing significantly to cellular interactions and positioning during development.
Biological function summary

CELSR1 is an important component of the planar cell polarity (PCP) signaling pathway. It acts as a part of a receptor complex that is important for the regulation of cell orientation and coordinated tissue patterning. This protein through its interaction with other PCP components helps in establishing cellular asymmetry and polarity within tissues. CELSR1 regulates developmental processes in various tissues making it a significant player in embryogenesis and organogenesis.

Pathways

CELSR1 integrates into the non-canonical Wnt/PCP signaling pathway influencing cellular processes such as migration and polarity. It interacts closely with proteins like Frizzled and Dishevelled to propagate signaling within this pathway. Additionally CELSR1's functions are connected with the Flamingo cadherins variously affecting cellular alignment and directional movement important for functioning tissues and organs.

Defects in CELSR1 can lead to congenital malformations such as neural tube defects and craniorachischisis. The protein's altered expression or dysfunction is linked to cancer development especially in tumors where cell polarity and adhesion are disrupted. CELSR1's relationship with proteins like Vangl and Dishevelled in disease contexts highlights its role in maintaining cellular integrity and tissue homeostasis and when perturbed contributes to pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor that may have an important role in cell/cell signaling during nervous system formation.
See full target information CELSR1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy oncolytics 23:355-366 PubMed34820505

2021

Knockdown of SENP1 inhibits HIF-1α SUMOylation and suppresses oncogenic CCNE1 in Wilms tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Shibo Zhu,Jinhua Hu,Yanhong Cui,Shen Liang,Xiaofeng Gao,Jin Zhang,Wei Jia

Cancer management and research 12:8857-8865 PubMed33061581

2020

CELSR1 Acts as an Oncogene Regulated by miR-199a-5p in Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Guang Wang,Yong Li,Dongxia Zhang,Songtao Zhao,Qiong Zhang,Chao Luo,Xiaochuan Sun,Bingqian Zhang
View all publications
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