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AB316239

Anti-CELSR1 antibody [EPR26770-76] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal CELSR1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

CDHF9, FMI2, CELSR1, Cadherin EGF LAG seven-pass G-type receptor 1, Cadherin family member 9, Flamingo homolog 2, hFmi2

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Sandwich ELISA - Anti-CELSR1 antibody [EPR26770-76] - BSA and Azide free (Detector) (AB316239)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-CELSR1 antibody [EPR26770-76] - BSA and Azide free (Detector) (AB316239)

Sandwich ELISA of ab316237 with the capture antibody ab316238 dilution at 2 ug/mL and detector antibody ab316239 dilution at 0.5 ug/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26770-76

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab316239 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human CELSR1. The recommended pair for sandwich ELISA is:

Capture: ab316238, Human CELSR1 Capture Antibody (unconjugated)

Detector: ab316239, Human CELSR1 Detector Antibody (unconjugated)

The reference range value is 39.06-2500 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CELSR1 also known as Cadherin EGF LAG seven-pass G-type receptor 1 is a member of the adhesion G-protein-coupled receptors family. Its molecular mass is approximately 350 kDa. The protein consists of several cadherin repeats and EGF-like domains playing a critical role in cell signaling and adhesion. CELSR1 is mainly expressed in the central nervous system and certain epithelial tissues contributing significantly to cellular interactions and positioning during development.
Biological function summary

CELSR1 is an important component of the planar cell polarity (PCP) signaling pathway. It acts as a part of a receptor complex that is important for the regulation of cell orientation and coordinated tissue patterning. This protein through its interaction with other PCP components helps in establishing cellular asymmetry and polarity within tissues. CELSR1 regulates developmental processes in various tissues making it a significant player in embryogenesis and organogenesis.

Pathways

CELSR1 integrates into the non-canonical Wnt/PCP signaling pathway influencing cellular processes such as migration and polarity. It interacts closely with proteins like Frizzled and Dishevelled to propagate signaling within this pathway. Additionally CELSR1's functions are connected with the Flamingo cadherins variously affecting cellular alignment and directional movement important for functioning tissues and organs.

Defects in CELSR1 can lead to congenital malformations such as neural tube defects and craniorachischisis. The protein's altered expression or dysfunction is linked to cancer development especially in tumors where cell polarity and adhesion are disrupted. CELSR1's relationship with proteins like Vangl and Dishevelled in disease contexts highlights its role in maintaining cellular integrity and tissue homeostasis and when perturbed contributes to pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor that may have an important role in cell/cell signaling during nervous system formation.
See full target information CELSR1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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