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AB92318

Anti-CEND1 antibody [EPR3738]

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Rabbit Recombinant Monoclonal CEND1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

View Alternative Names

BM88, CEND1, Cell cycle exit and neuronal differentiation protein 1, BM88 antigen

2 Images
Western blot - Anti-CEND1 antibody [EPR3738] (AB92318)
  • WB

Unknown

Western blot - Anti-CEND1 antibody [EPR3738] (AB92318)

All lanes:

Western blot - Anti-CEND1 antibody [EPR3738] (ab92318) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Neuro-2a cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 15 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEND1 antibody [EPR3738] (AB92318)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEND1 antibody [EPR3738] (AB92318)

ab92318 at a 1/100 dilution staining CEND1 in paraffin-embedded Human brain tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Carrier free

    Anti-CEND1 antibody [EPR3738] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3738

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.31% Sodium citrate, 0.175% Sodium chloride, 0.05% BSA, 0.0172% EDTA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEND1 also known as cell cycle exit and neuronal differentiation 1 is a protein with a mass of approximately 16 kDa. Researchers have identified its expression specifically in neural precursor cells of the central nervous system. CEND1 plays a role in controlling neuronal differentiation by interacting with cell cycle regulators. This function highlights its importance in the development of the nervous system and its involvement in maintaining the balance between proliferation and differentiation in neurons.
Biological function summary

CEND1 facilitates neuronal outgrowth and maturation without forming part of a larger multiprotein complex. It acts directly on governing neuronal progenitor cell fate promoting differentiation over proliferation. Researchers have found CEND1 to affect cell cycle arrest in neuronal populations steering progenitor cells towards post-mitotic states essential for healthy neural tissue maturation. The presence of CEND1 in neurogenesis stages establishes it as an important player in the shaping of the neuronal landscape in the brain.

Pathways

Several cell cycle-related pathways involve CEND1 in regulating the transition from proliferation to differentiation in neural progenitors. It interacts with key proteins such as p27^Kip1 and cyclin-dependent kinases. These interactions are important in coordinating neurogenesis and neuronal pathway specification. CEND1 ensures proper function and cooperation with other elements of the neurogenic program including pathways like Notch signaling to regulate the temporal and spatial aspects of neuronal differentiation.

Research has linked CEND1 to neurodevelopmental conditions such as microcephaly and certain forms of epilepsy. Malfunctions or dysregulation of CEND1 can result in impaired neuronal differentiation leading to disturbed neural circuit formation. In these contexts CEND1 interacts with proteins like p53 which governs cell cycle arrest and apoptosis offering insights into how disrupted CEND1 expression contributes to the pathophysiology of these neurological disorders. Understanding these relationships provides potential therapeutic targets for correcting or mitigating such conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in neuronal differentiation.
See full target information CEND1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com