Anti-Cenexin1/ODF2 antibody

• ICC/IF

AbReview58215****

Immunocytochemistry/ Immunofluorescence - Anti-Cenexin1/ODF2 antibody (AB43840)

Immunocytochemistry/ Immunofluorescence analysis of human HCT116 cells labeling Cenexin1/ODF2 with ab43840 at 1/400 dilution. Cells were fixed in formaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Staining with ab43840 at 1/400 was carried out for 1 hour at 22°C in PBS buffer. ab150081, a Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed secondary antibody was used at 1/200 dilution. DAPI was used to counterstain.

This image is courtesy of an Abreview submitted by Kirk McManus, Univ. of Manitoba/Cancer Care MICB.

• WB

Lab

Western blot - Anti-Cenexin1/ODF2 antibody (AB43840)

Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

ab43840 at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 25 µg

Lane 2:

MCF7 (Human breast adenocarcinoma) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

Predicted band size: 95 kDa

Observed band size: 100 kDa

true

Exposure time: 20min

• WB

AbReview17722****

Western blot - Anti-Cenexin1/ODF2 antibody (AB43840)

Detection of Cenexin 1 by ab43840 in Western blot of HeLa cell lysates. Please see anonymous abreview for further details.

All lanes:

Western blot - Anti-Cenexin1/ODF2 antibody (ab43840) at 1/500 dilution

Lane 1:

25 ug HeLa cell lysate

Lane 2:

12.5 ug HeLa cell lysate

Lane 3:

25 ug HeLa cell lysate with Human Cenexin1/ODF2 peptide (<a href='/en-us/products/unavailable/human-cenexin1odf2-peptide-ab43839'>ab43839</a>)

Lane 4:

12.5 ug HeLa cell lysate with Human Cenexin1/ODF2 peptide (<a href='/en-us/products/unavailable/human-cenexin1odf2-peptide-ab43839'>ab43839</a>)

Secondary

All lanes:

Anti-Rabbit IgG HRP-conjugated at 1/5000 dilution

Predicted band size: 95 kDa

Observed band size: ~75 kDa

true

Exposure time: 4min

Image courtesy of an anonymous abreview.