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AB250874

Anti-Cenexin1/ODF2 antibody [EPR13561] - BSA and Azide free

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Rabbit Recombinant Monoclonal Cenexin1/ODF2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Outer dense fiber protein 2, Cenexin, Outer dense fiber of sperm tails protein 2, ODF2

3 Images
Flow Cytometry (Intracellular) - Anti-Cenexin1/ODF2 antibody [EPR13561] - BSA and Azide free (AB250874)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cenexin1/ODF2 antibody [EPR13561] - BSA and Azide free (AB250874)

This data was developed using ab186412, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of paraformaldehyde fixed Hela cells labeling Cenexin1/ODF2 with ab186412 at 1/250 dilution and Goat anti-rabbit IgG (FITC) at 1/150 dilution

Western blot - Anti-Cenexin1/ODF2 antibody [EPR13561] - BSA and Azide free (AB250874)
  • WB

Supplier Data

Western blot - Anti-Cenexin1/ODF2 antibody [EPR13561] - BSA and Azide free (AB250874)

This data was developed using ab186412, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cenexin1/ODF2 antibody [EPR13561] (<a href='/en-us/products/primary-antibodies/cenexin1-odf2-antibody-epr13561-ab186412'>ab186412</a>) at 1/1000 dilution

All lanes:

Human testis lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 95 kDa

false

Western blot - Anti-Cenexin1/ODF2 antibody [EPR13561] - BSA and Azide free (AB250874)
  • WB

Supplier Data

Western blot - Anti-Cenexin1/ODF2 antibody [EPR13561] - BSA and Azide free (AB250874)

This data was developed using ab186412, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cenexin1/ODF2 antibody [EPR13561] (<a href='/en-us/products/primary-antibodies/cenexin1-odf2-antibody-epr13561-ab186412'>ab186412</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

Hela cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 95 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13561

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab250874 is the carrier-free version of ab186412.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.
See full target information Outer dense fiber protein 2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB43840

Anti-Cenexin1/ODF2 antibody

primary-antibodies

cenexin1-odf2-antibody-ab43840

4

(4 reviews)

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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