Rabbit Polyclonal CENH3 antibody. Suitable for ICC, WB and reacts with Arabidopsis thaliana samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Arabidopsis thaliana CENH3.
Constituents: Whole serum
| ICC | WB | |
|---|---|---|
| Arabidopsis thaliana | Expected | Expected |
| Species | Dilution info | Notes |
|---|---|---|
Species Arabidopsis thaliana | Dilution info Use at an assay dependent concentration. | Notes - |
| Species | Dilution info | Notes |
|---|---|---|
Species Arabidopsis thaliana | Dilution info Use at an assay dependent concentration. | Notes - |
Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division (By similarity).
HTR12, At1g01370, F6F3.17, CENH3, Histone H3-like centromeric protein CENH3, Centromeric histone CENH3, Centromeric histone H3, Histone H3-like centromeric protein HTR12
Rabbit Polyclonal CENH3 antibody. Suitable for ICC, WB and reacts with Arabidopsis thaliana samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Arabidopsis thaliana CENH3.
Constituents: Whole serum
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CENH3 also known as Centromere Protein A is a histone H3 variant that plays an essential role in the packaging of centromeric DNA. This protein has a mass of approximately 17 kDa and is uniquely expressed in the centromeric chromatin of eukaryotic cells. CENH3 differs from the canonical H3 histone by having a distinct N-terminal tail which is important for its specific incorporation into centromeres. This specific localization is necessary for the recognition and binding of other centromeric proteins facilitating the assembly of the kinetochore complex.
CENH3 integrates into nucleosomes at the centromeres forming a specialized chromatin structure critical for chromosome segregation. It contributes to the foundation of the centromere by assembling into a larger protein complex known as the kinetochore. This complex mediates the attachment of spindle microtubules during mitosis ensuring accurate distribution of chromosomes to daughter cells. CENH3's presence at the centromeres helps in recruiting other centromeric proteins such as CENP-C and CENP-N highlighting its central role in stabilizing the centromeric region.
CENH3 participates in the cell cycle regulation specifically during mitosis. It acts within the mitotic spindle assembly checkpoint pathway ensuring proper kinetochore function and tension sensing. The mitotic checkpoint kinase MPS1 phosphorylates proteins at the kinetochore and stable CENH3 positioning is necessary for correct attachment. The proper function of CENH3 influences pathways connected to Aurora B kinase activity which affects the correction of misattached microtubules ensuring accurate and efficient mitotic progression.
CENH3 abnormalities can contribute to conditions connected to chromosome missegregation and aneuploidy. Misregulation of CENH3 expression or function links to developmental disorders like cancer and congenital disorders. Dysregulated CENH3 may impact proteins such as SGO1 an essential kinetochore protein that directly affects chromosome cohesion and separation. Abnormal CENH3 expression can result in improper kinetochore function leading to an increased risk of chromosomal instability and tumorigenesis.
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