AB72001

Anti-CENH3 antibody

Name: Anti-CENH3 antibody (ab72001) | Abcam
Brand: Abcam Limited
SKU: AB72001

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(10 Publications)

Rabbit Polyclonal CENH3 antibody. Suitable for ICC, WB and reacts with Arabidopsis thaliana samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Arabidopsis thaliana CENH3.

View Alternative Names

HTR12, At1g01370, F6F3.17, CENH3, Histone H3-like centromeric protein CENH3, Centromeric histone CENH3, Centromeric histone H3, Histone H3-like centromeric protein HTR12

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Arabidopsis thaliana

Applications

ICC, WB

applications

Immunogen

Synthetic Peptide within Arabidopsis thaliana CENH3. The exact immunogen used to generate this antibody is proprietary information.

Q8RVQ9

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Reactivity data

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CENH3 also known as Centromere Protein A is a histone H3 variant that plays an essential role in the packaging of centromeric DNA. This protein has a mass of approximately 17 kDa and is uniquely expressed in the centromeric chromatin of eukaryotic cells. CENH3 differs from the canonical H3 histone by having a distinct N-terminal tail which is important for its specific incorporation into centromeres. This specific localization is necessary for the recognition and binding of other centromeric proteins facilitating the assembly of the kinetochore complex.

Biological function summary

CENH3 integrates into nucleosomes at the centromeres forming a specialized chromatin structure critical for chromosome segregation. It contributes to the foundation of the centromere by assembling into a larger protein complex known as the kinetochore. This complex mediates the attachment of spindle microtubules during mitosis ensuring accurate distribution of chromosomes to daughter cells. CENH3's presence at the centromeres helps in recruiting other centromeric proteins such as CENP-C and CENP-N highlighting its central role in stabilizing the centromeric region.

Pathways

CENH3 participates in the cell cycle regulation specifically during mitosis. It acts within the mitotic spindle assembly checkpoint pathway ensuring proper kinetochore function and tension sensing. The mitotic checkpoint kinase MPS1 phosphorylates proteins at the kinetochore and stable CENH3 positioning is necessary for correct attachment. The proper function of CENH3 influences pathways connected to Aurora B kinase activity which affects the correction of misattached microtubules ensuring accurate and efficient mitotic progression.

CENH3 abnormalities can contribute to conditions connected to chromosome missegregation and aneuploidy. Misregulation of CENH3 expression or function links to developmental disorders like cancer and congenital disorders. Dysregulated CENH3 may impact proteins such as SGO1 an essential kinetochore protein that directly affects chromosome cohesion and separation. Abnormal CENH3 expression can result in improper kinetochore function leading to an increased risk of chromosomal instability and tumorigenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division (By similarity).

See full target information CENH3

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

The Plant cell 37: PubMed40244922

2025

ANAPHASE-PROMOTING COMPLEX/CYCLOSOME coactivators maintain AURORA 1 kinase homeostasis during meiotic chromosome segregation.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xu,Lian Zhou,Kaixin Chen,Runsen Huang,Baixiao Niu,Juanying Ye,Hong Ma,Gregory P Copenhaver,Yingxiang Wang

Nature genetics 57:986-996 PubMed40140587

2025

The genome and GeneBank genomics of allotetraploid Nicotiana tabacum provide insights into genome evolution and complex trait regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjun Zan,Shuai Chen,Min Ren,Guoxiang Liu,Yutong Liu,Yu Han,Yang Dong,Yao Zhang,Huan Si,Zhengwen Liu,Dan Liu,Xingwei Zhang,Ying Tong,Yuan Li,Caihong Jiang,Liuying Wen,Zhiliang Xiao,Yangyang Sun,Ruimei Geng,Yan Ji,Quanfu Feng,Yuanying Wang,Guoyou Ye,Lingzhao Fang,Yong Chen,Lirui Cheng,Aiguo Yang

Journal of integrative plant biology 66:2208-2225 PubMed39109967

2024

The pineapple reference genome: Telomere-to-telomere assembly, manually curated annotation, and comparative analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Junting Feng,Wei Zhang,Chengjie Chen,Yinlong Liang,Tangxiu Li,Ya Wu,Hui Liu,Jing Wu,Wenqiu Lin,Jiawei Li,Yehua He,Junhu He,Aiping Luan

Plant molecular biology 114:74 PubMed38874679

2024

Centromere sequence-independent but biased loading of subgenome-specific CENH3 variants in allopolyploid Arabidopsis suecica.

Applications

Unspecified application

Species

Unspecified reactive species

Raheleh Karimi-Ashtiyani,Ali Mohammad Banaei-Moghaddam,Takayoshi Ishii,Oda Weiss,Jörg Fuchs,Veit Schubert,Andreas Houben

Science (New York, N.Y.) 374:eabi7489 PubMed34762468

2021

The genetic and epigenetic landscape of the centromeres.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Naish,Michael Alonge,Piotr Wlodzimierz,Andrew J Tock,Bradley W Abramson,Anna Schmücker,Terezie Mandáková,Bhagyshree Jamge,Christophe Lambing,Pallas Kuo,Natasha Yelina,Nolan Hartwick,Kelly Colt,Lisa M Smith,Jurriaan Ton,Tetsuji Kakutani,Robert A Martienssen,Korbinian Schneeberger,Martin A Lysak,Frédéric Berger,Alexandros Bousios,Todd P Michael,Michael C Schatz,Ian R Henderson

PLoS genetics 17:e1009779 PubMed34591845

2021

A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Claudio Capitao,Sorin Tanasa,Jaroslav Fulnecek,Vivek K Raxwal,Svetlana Akimcheva,Petra Bulankova,Pavlina Mikulkova,Lucie Crhak Khaitova,Manikandan Kalidass,Inna Lermontova,Ortrun Mittelsten Scheid,Karel Riha

The New phytologist 224:229-241 PubMed31230348

2019

The Arabidopsis anaphase-promoting complex/cyclosome subunit 8 is required for male meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Rong-Yan Xu,Jing Xu,Liudan Wang,Baixiao Niu,Gregory P Copenhaver,Hong Ma,Binglian Zheng,Yingxiang Wang

Proceedings of the National Academy of Sciences of 112:11211-6 PubMed26294252

2015

Point mutation impairs centromeric CENH3 loading and induces haploid plants.

Applications

Species

Unspecified reactive species

Raheleh Karimi-Ashtiyani,Takayoshi Ishii,Markus Niessen,Nils Stein,Stefan Heckmann,Maia Gurushidze,Ali Mohammad Banaei-Moghaddam,Jörg Fuchs,Veit Schubert,Kerstin Koch,Oda Weiss,Dmitri Demidov,Klaus Schmidt,Jochen Kumlehn,Andreas Houben

The New phytologist 208:1067-77 PubMed26213803

2015

Mitotic lifecycle of chromosomal 3xHMG-box proteins and the role of their N-terminal domain in the association with rDNA loci and proteolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Antosch,Veit Schubert,Philipp Holzinger,Andreas Houben,Klaus D Grasser

Proceedings of the National Academy of Sciences of 111:16166-71 PubMed25344531

2014

The AAA-ATPase molecular chaperone Cdc48/p97 disassembles sumoylated centromeres, decondenses heterochromatin, and activates ribosomal RNA genes.

Applications

Unspecified application

Species

Unspecified reactive species

Zsuzsanna Mérai,Nina Chumak,Marcelina García-Aguilar,Tzung-Fu Hsieh,Toshiro Nishimura,Vera K Schoft,János Bindics,Lucyna Slusarz,Stéphanie Arnoux,Susanne Opravil,Karl Mechtler,Daniel Zilberman,Robert L Fischer,Hisashi Tamaru

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CENH3 antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise