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AB189957

Anti-CENP-W antibody - N-terminal

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(1 Publication)

Rabbit Polyclonal CENP-W antibody. N-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CENPW.

View Alternative Names

C6orf173, CUG2, CENPW, Centromere protein W, CENP-W, Cancer-up-regulated gene 2 protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENP-W antibody - N-terminal (AB189957)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENP-W antibody - N-terminal (AB189957)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human spleen sections labeling CENP-W with ab189957 at 5 μg/ml.

Western blot - Anti-CENP-W antibody - N-terminal (AB189957)
  • WB

Supplier Data

Western blot - Anti-CENP-W antibody - N-terminal (AB189957)

Lane 1:

Western blot - Anti-CENP-W antibody - N-terminal (ab189957) at 0.5 µg/mL

Lane 2:

Western blot - Anti-CENP-W antibody - N-terminal (ab189957) at 1 µg/mL

All lanes:

HeLa cell lysate

Predicted band size: 10 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human CENPW. The exact immunogen used to generate this antibody is proprietary information.

Q5EE01

Specificity

At least three isoforms of CENP-W are known to exist; ab189957 will detect all three isoforms.

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Complementary Products

Primary Antibodies

AB220280

Anti-CENPT antibody

Immunocytochemistry/ Immunofluorescence - Anti-CENPT antibody (AB220280)

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Primary Antibodies

AB105742

Anti-CENPQ antibody

Immunocytochemistry/ Immunofluorescence - Anti-CENPQ antibody (AB105742)

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Primary Antibodies

AB118796

Anti-CENPI antibody

Western blot - Anti-CENPI antibody (AB118796)

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Primary Antibodies

AB25734

Anti-CENPB antibody

KO Validated

Western blot - Anti-CENPB antibody (AB25734)

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  • 6
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CENP-W also known as Centromere Protein W is a component of the CENP-A nucleosome-associated complex (NAC) and it plays an important role in centromere structure and function. This protein has a molecular weight of approximately 19 kDa. CENP-W is mostly expressed in the nucleus where it functions at the centromere. This protein is a part of the larger centromere complex and is essential in maintaining the stability of kinetochore microtubule attachments during cell division.
Biological function summary

CENP-W is necessary for the correct assembly of the kinetochore complex. It partners with other proteins within the NAC such as CENP-X and CENP-T to ensure that the centromere is properly structured for chromosomal segregation. CENP-W directly interacts with CENP-A to facilitate the recruitment of other centromere proteins ensuring cohesion and accurate segregation of chromosomes. This activity is critical for maintaining genomic stability during cell replication and division.

Pathways

CENP-W is significant in the cell cycle checkpoint regulation and mitotic pathways. CENP-W contributes to the proper activation of the spindle assembly checkpoint a critical quality control step that ensures chromosomes are correctly aligned before cell division proceeds. It interacts with proteins like CENP-A and CENP-B to control and regulate these pathways ensuring that the cell progresses through mitosis with high fidelity.

Dysfunction of CENP-W is associated with various cancers. Aberrations in centromere or kinetochore function to which CENP-W is central can lead to chromosomal instability a hallmark of many tumors. CENP-W's relationship with CENP-T and CENP-A also links it to disorders characterized by aneuploidy a condition where cells have an abnormal number of chromosomes. Understanding the role of CENP-W and its interactions in these diseases may provide new avenues for therapeutic strategies targeting chromosomal segregation mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation (By similarity). The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres (By similarity). Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPW has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.
See full target information CENPW
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Technology in cancer research & treatment 18:1533033819874783 PubMed31526099

2019

Zinc Finger Protein 521, Negatively Regulated by MicroRNA-204-5p, Promotes Proliferation, Motility and Invasion of Gastric Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Huan,Cai Xiaoxu,Ren Xifang
View all publications
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Product promise

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