Anti-CENPA antibody [EP800Y] - Centromere Marker

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (AB45694)

Immunohistochemical analysis of formalin fixed, paraffin embedded Lymphoma tissue section labelling CENPA with unpurified ab45694.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (AB45694)

IHC image of ab45694 staining CENPA in Human skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with unpurified ab45694, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (AB45694)

Immunohistochemical analysis of formalin fixed, paraffin embedded prostatic carcinoma tissue section labelling CENPA with unpurified ab45694.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (AB45694)

Immunohistochemical analysis of paraffin embedded human colon tissue section labelling CENPA with purified ab45694 at dilution of 1/250. The secondary antibody used was HRP-conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

• WB

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Western blot - Anti-CENPA antibody [EP800Y] - Centromere Marker (AB45694)

Blocking and diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Human melanoma at 20 µg

Lane 3:

A375 (human malignant melanoma) whole cell lysate at 20 µg

Lane 4:

HCT-116 (human colorectal carcinoma) whole cell lysate at 20 µg

Lane 5:

Human placenta lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 16 kDa

Observed band size: 18 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (AB45694)

Immunohistochemical analysis of formalin fixed, paraffin embedded lung carcinoma tissue section labelling CENPA with unpurified ab45694.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (AB45694)

Immunohistochemical analysis of formalin fixed, paraffin embedded colonic adenocarcinoma tissue section labelling CENPA with unpurified ab45694.

• WB

Unknown

Western blot - Anti-CENPA antibody [EP800Y] - Centromere Marker (AB45694)

All lanes:

Western blot - Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694) at 1/20000 dilution

All lanes:

HeLa cell lysate (10ug/lane)

Predicted band size: 16 kDa

Observed band size: 18 kDa

false