JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB62242

Anti-CENPA (phospho S7) antibody

5

(1 Review)

|

(2 Publications)

Rabbit Polyclonal CENPA phospho S7 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CENPA phospho S7 aa 1-50.

View Alternative Names

Histone H3-like centromeric protein A, Centromere autoantigen A, Centromere protein A, CENP-A, CENPA

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CENPA (phospho S7) antibody (AB62242)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CENPA (phospho S7) antibody (AB62242)

ab62242 at 1/500 - 1/1000 dilution staining CENPA in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Immunocytochemistry/ Immunofluorescence - Anti-CENPA (phospho S7) antibody (AB62242)
  • ICC/IF

AbReview60913****

Immunocytochemistry/ Immunofluorescence - Anti-CENPA (phospho S7) antibody (AB62242)

ab62242 staining CENPA (phospho S7) in the Hela cell line from Human cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Methanol. Samples were incubated with primary antibody (1/400 in PBS) for 1 hour at 22°C. Undiluted ab150081 was used as the secondary antibody.

This image is courtesy of an abreview by Kirk Mcmanus

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human CENPA phospho S7 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P49450

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab62242 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CENPA also known as Centromere Protein A is an essential component of the centromere specifically during cell division. It represents a histone H3 variant with an approximate mass of 17 kDa. This protein is expressed at the centromere regions of chromosomes where it substitutes conventional histone H3. CENPA facilitates the assembly of the kinetochore an important structure for chromosome segregation. Scientists also refer to this protein as CENP-A often in conjunction with studies involving anti-centromere antibodies and centromere staining.
Biological function summary

CENPA plays a central role in accurate chromosome segregation during mitosis and meiosis. It incorporates into nucleosomes at the centromere replacing standard histone H3 to promote kinetochore formation. CENPA is part of a larger protein complex known as the centromere. This complex propelled by CENPA drives proper attachment of chromosomes to spindle microtubules preventing aneuploidy.

Pathways

The kinetochore pathway and the chromosome segregation pathway both involve CENPA significantly. It functions in tandem with other centromeric proteins such as CENP-B and CENP-C which work collaboratively to sustain centromere integrity. CENPA interacts with these proteins to orchestrate correct alignment and separation of chromosomes integrating it into the foundational architecture of genetic stability.

CENPA has association with several chromosomal instability syndromes. Misregulation or mutation of this protein may contribute to cancer progression where improper chromosome segregation is an important factor. It also connects to other proteins like CENP-H implicated in malignancies due to errors in chromosome transmission. Additionally autoimmune diseases often involve centromere antibodies highlighting the connection of CENPA to disorders such as systemic sclerosis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes (PubMed : 11756469, PubMed : 14667408, PubMed : 15282608, PubMed : 15475964, PubMed : 15702419, PubMed : 17651496, PubMed : 19114591, PubMed : 20739937, PubMed : 27499292, PubMed : 7962047, PubMed : 9024683). Replaces conventional H3 in the nucleosome core of centromeric chromatin that serves as an assembly site for the inner kinetochore (PubMed : 18072184). The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3 (PubMed : 26878239, PubMed : 27499292). May serve as an epigenetic mark that propagates centromere identity through replication and cell division (PubMed : 15282608, PubMed : 15475964, PubMed : 20739937, PubMed : 21478274, PubMed : 26878239). Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis (PubMed : 11756469, PubMed : 14667408, PubMed : 18072184, PubMed : 23818633, PubMed : 25556658, PubMed : 27499292).
See full target information CENPA phospho S7
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 121:e2405015121 PubMed39541344

2024

Microtubule poleward flux as a target for modifying chromosome segregation errors.

Applications

Unspecified application

Species

Unspecified reactive species

Patrik Risteski,Jelena Martinčić,Mihaela Jagrić,Erna Tintor,Ana Petelinec,Iva M Tolić

The Journal of cell biology 190:73-87 PubMed20624901

2010

Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine.

Applications

WB, ICC/IF

Species

Human, Human

Stefano Santaguida,Anthony Tighe,Anna Morena D'Alise,Stephen S Taylor,Andrea Musacchio
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB217622

Anti-CENPA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody (AB217622)

  • 1
  • 1
Primary Antibodies

AB45694

Anti-CENPA antibody [EP800Y] - Centromere Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (AB45694)

  • 1
  • 2

Complementary Products

Primary Antibodies

AB33911

Anti-Cyclin E1 antibody [EP435E]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 antibody [EP435E] (AB33911)

  • 4
  • 8
Primary Antibodies

AB190908

Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473]

RabMAb|Recombinant|Advanced Validation|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (AB190908)

  • 0
  • 0
Primary Antibodies

AB133534

Anti-Rad51 antibody [EPR4030(3)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [EPR4030(3)] (AB133534)

  • 4
  • 15
Primary Antibodies

AB89901

Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (AB89901)

  • 5
  • 43

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com