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AB167361

Anti-CENPB antibody

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(3 Publications)

Mouse Polyclonal CENPB antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CENPB.

View Alternative Names

Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB

3 Images
Western blot - Anti-CENPB antibody (AB167361)
  • WB

Unknown

Western blot - Anti-CENPB antibody (AB167361)

All lanes:

Western blot - Anti-CENPB antibody (ab167361) at 1 µg/mL

All lanes:

A431 cell lysate at 50 µg

Secondary

All lanes:

Goat anti-Mouse IgG HRP at 1/2500 dilution

Predicted band size: 65 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (AB167361)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (AB167361)

Immunofluorescence analysis of HeLa cells labeling CENPB using ab167361 at 10 μg/ml.

Western blot - Anti-CENPB antibody (AB167361)
  • WB

Unknown

Western blot - Anti-CENPB antibody (AB167361)

All lanes:

Western blot - Anti-CENPB antibody (ab167361) at 1 µg/mL

Lane 1:

CENPB transfected 293T cell line lysate at 15 µL

Lane 2:

Non-transfected 293T cell line lysate at 15 µL

Secondary

All lanes:

Goat anti-Mouse IgG HRP at 1/2500 dilution

Predicted band size: 65 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CENPB.

P07199

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CENP-B also known as Centromere Protein B is a protein with a molecular weight of approximately 80 kDa. It binds to the centromeric DNA and plays a critical role in centromere formation and function. CENP-B is heavily expressed in dividing cells particularly in tissues where cell proliferation is high such as bone marrow and epithelial tissues. Researchers often use tools like anti-CENP-B antibodies and CENP-B ELISA kits to study its expression in various experimental settings.
Biological function summary

CENP-B participates in the assembly of the centromere protein complex contributing to the stability and maintenance of the centromere structure during cell division. It acts as a foundation for kinetochore nucleation ensuring proper chromosome segregation. CENP-B is involved in the formation of the CENP-A nucleosome complex important for accurate chromosomal alignment and separation.

Pathways

CENP-B is essential in the mitotic spindle assembly and chromosome segregation pathways. This protein interacts with CENP-A a variant histone which integrates into centromeric nucleosomes to initiate the formation of functional kinetochores. Additionally CENP-B interacts with CENP-C facilitating the recruitment of other centromere-associated proteins needed for chromosome dynamics during mitosis.

CENP-B is primarily associated with autoimmune disorders such as systemic sclerosis (scleroderma). Patients with these conditions often produce anti-CENP-B antibodies which serve as diagnostic markers. Moreover CENP-B's dysregulation links to chromosomal instability a contributor to cancer. Research has shown connections between CENP-B and related proteins like CENP-A in the context of chromosomal aneuploidies and malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box (PubMed : 11726497). May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes (Probable).
See full target information CENPB
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 12:144 PubMed39227882

2024

ALS-associated C21ORF2 variant disrupts DNA damage repair, mitochondrial metabolism, neuronal excitability and NEK1 levels in human motor neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Pavol Zelina,Anna Aster de Ruiter,Christy Kolsteeg,Ilona van Ginneken,Harmjan R Vos,Laura F Supiot,Boudewijn M T Burgering,Frank J Meye,Jan H Veldink,Leonard H van den Berg,R Jeroen Pasterkamp

The Journal of cell biology 220: PubMed33443568

2021

Induction of spontaneous human neocentromere formation and long-term maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Murillo-Pineda,Luis P Valente,Marie Dumont,João F Mata,Daniele Fachinetti,Lars E T Jansen

Nature communications 11:501 PubMed31980633

2020

Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Sreyoshi Mitra,Dani L Bodor,Ana F David,Izma Abdul-Zani,João F Mata,Beate Neumann,Sabine Reither,Christian Tischer,Lars E T Jansen
View all publications
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Product promise

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