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Rabbit Polyclonal CENPB antibody. Suitable for ICC/IF, ICC, WB and reacts with Human samples. Cited in 46 publications.


Images

Immunocytochemistry - Anti-CENPB antibody (AB25734), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (AB25734), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (AB25734), expandable thumbnail
  • Western blot - Anti-CENPB antibody (AB25734), expandable thumbnail
  • Western blot - Anti-CENPB antibody (AB25734), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFICCWB
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Hamster
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info
1/200.00000 - 1/1000.00000
Notes

-

Predicted
Predicted

Species

Mouse, Rat, Sheep, Hamster

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species

Mouse, Rat, Sheep, Hamster

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info
1.00000-5.00000 µg/mL
Notes

-

Predicted
Predicted

Species

Mouse, Rat, Sheep, Hamster

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box (PubMed:11726497). May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes (Probable).

Alternative names

Recommended products

Rabbit Polyclonal CENPB antibody. Suitable for ICC/IF, ICC, WB and reacts with Human samples. Cited in 46 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

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5 product images

  • Immunocytochemistry - Anti-CENPB antibody (ab25734), expandable thumbnail
    This image is courtesy of a customer review submitted by Dr Kirk McManus

    Immunocytochemistry - Anti-CENPB antibody (ab25734)

    ab25734 (1/1000) staining CENPB in HeLa cells (green). Cells were fixed with methanol and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

  • Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (ab25734), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (ab25734)

    ab25734 staining CENPB in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab25734 at 1 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 100% methanol (5 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (ab25734), expandable thumbnail
    Image courtesy of a customer review by Weiguo Zhang

    Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (ab25734)

    4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CENPB (red) using ab25734 at 1/200 dilution in ICC/IF.

    The nuclear counterstain is DAPI (blue). Alpha-Tubulin is stained with an mouse monoclonal anti-alpha Tubulin antibody (green).

  • Western blot - Anti-CENPB antibody (ab25734), expandable thumbnail

    Western blot - Anti-CENPB antibody (ab25734)

    All lanes: Western blot - Anti-CENPB antibody (ab25734) at 1 µg/mL

    Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Lane 2: Western blot - Jurkat whole cell lysate (Jurkat whole cell lysate ab7899) at 20 µg

    Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 65 kDa

    Observed band size: 65 kDa, 74 kDa, 75 kDa

  • Western blot - Anti-CENPB antibody (ab25734), expandable thumbnail

    Western blot - Anti-CENPB antibody (ab25734)

    Lanes 1 - 4: Merged signal (red and green). Green - ab25734 observed at 77 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab25734 was shown to react with CENPB in wild-type A431 cells in Western blot with loss of signal observed in CENPB knockout sample.Wild-type A431 and CENPB knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab25734 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-CENPB antibody (ab25734) at 1 µg/mL

    Lane 1: Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: CENPB knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 4: Daudi (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 65 kDa

    Observed band size: 77 kDa

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Product protocols

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