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AB264252

Anti-CENPE antibody

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(1 Publication)

Rabbit Polyclonal CENPE antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CENPE aa 2500 to C-terminus.

View Alternative Names

Centromere-associated protein E, Centromere protein E, Kinesin-7, Kinesin-related protein CENPE, CENP-E, CENPE

1 Images
Immunoprecipitation - Anti-CENPE antibody (AB264252)
  • IP

Supplier Data

Immunoprecipitation - Anti-CENPE antibody (AB264252)

CENPE was immunoprecipitated from 1mg of HeLa whole cell lysates using ab264252 at 3μg/mg. Western blot was performed on the immunprecipitates using a different CENPE antibody at 1μg/ml.

Lane 1 : ab264252 IP in HeLa whole cell lysate.

lane 2 : Control IgG IP in HeLa whole cell lysate.

Detected with chemilimuniscence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-CENPE antibody (ab264252)

Predicted band size: 316 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human CENPE aa 2500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q02224

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CENPE also known as centromere-associated protein E or kinesin-like protein CENP-E plays a critical role in the alignment of chromosomes during mitosis. This protein with a molecular mass of approximately 312 kDa acts as a motor protein involved in chromosome movement. CENPE is mainly expressed in dividing cells and plays an important role during the metaphase stage of the cell cycle. The protein is localized at the kinetochores the protein complexes on chromatids where the spindle fibers attach during cell division.
Biological function summary

CENPE is a vital component for chromosome congression which ensures proper chromosome alignment and segregation. It is part of several essential complexes necessary for mitotic checkpoint signaling and the correction of chromosome-microtubule attachments. Without CENPE's function cells face increased risks of aneuploidy. The protein interacts with microtubules to drive the movement toward the center of the cell and is important in ensuring accurate cell division.

Pathways

CENPE is involved in the mitotic checkpoint and spindle assembly pathways. These pathways ensure the correct distribution of chromosomes during cell division and prevent genetic instability. CENPE interacts with other proteins such as BUBR1 and Mad2 which are integral parts of the mitotic checkpoint complex. Proper functioning of CENPE ensures the activation of these checkpoints preventing premature separation of chromatids and maintaining genomic integrity.

CENPE has links to various conditions characterized by chromosomal instability such as cancer. Aberrant expression or dysfunction of CENPE can lead to improper chromosome segregation contributing to aneuploidy a hallmark of many cancers. Moreover its function connects with other proteins like Aurora B kinase which also plays a role in chromosomal alignment and stability. Disruption in the functions of these proteins can promote tumor progression and malignancy.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules (PubMed : 23891108, PubMed : 25395579, PubMed : 7889940). The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated (PubMed : 25908662). Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends (PubMed : 23955301). Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized (PubMed : 25743205). Plays an important role in the formation of stable attachments between kinetochores and spindle microtubules (PubMed : 17535814) The stabilization of kinetochore-microtubule attachment also requires CENPE-dependent localization of other proteins to the kinetochore including BUB1B, MAD1 and MAD2. Plays a role in spindle assembly checkpoint activation (SAC) via its interaction with BUB1B resulting in the activation of its kinase activity, which is important for activating SAC. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss (By similarity).
See full target information CENPE
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 18:e1010588 PubMed35709296

2022

RANBP2 and USP9x regulate nuclear import of adenovirus minor coat protein IIIa.

Applications

Unspecified application

Species

Unspecified reactive species

Ashrafali M Ismail,Amrita Saha,Ji S Lee,David F Painter,Yinghua Chen,Gurdeep Singh,Gabriela N Condezo,James Chodosh,Carmen San Martín,Jaya Rajaiya
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